Molecular and microbial insights towards anaerobic biodegradation of anionic polyacrylamide in oil sands tailings.

Anionic polyacrylamide (A-PAM) is widely used as a flocculant in the management of oil sands tailings. Nevertheless, apprehensions arise regarding its potential biodegradation and environmental consequences within the context of oil sands tailings. Consequently, it is imperative to delve into the anaerobic biodegradation of A-PAM in oil sands tailings to gain a comprehensive understanding of its influence on tailings water quality. This work explored the dynamics of A-PAM biodegradation across concentrations: 50, 100, 250, 500, 1000, and 2000 mg/kg TS. The results showed a significant decrease in A-PAM concentration and molecular weight at lower concentrations (50 and 100 mg/kg TS) compared to higher ones, suggesting enhanced degradation efficiency. Likewise, the organic transformation and methane production exhibited dependency on A-PAM concentrations. The peak concentrations observed were 20.0 mg/L for volatile fatty acids (VFAs), 0.07 mg/L for acrylamide (AMD), and 8.9 mL for methane yield, with these maxima being recorded at 50 mg/kg TS. The biodegradation efficiency diminishes at higher concentrations of A-PAM, potentially due to the inhibitory effects of polyacrylic acid accumulation. A-PAM biodegradation under anaerobic condition did not contribute to acute toxicity or genotoxicity. SEM-EDS, FT-IR and XRD analyses further revealed that higher concentrations of A-PAM inhibited the biodegradation by altering floc structure and composition, thereby restricting the microbial activity. Major microorganisms, including Smithella, Candidatus_Cloacimonas, W5, XBB1006, and DMER64 were identified, highlighting A-PAM's dual role as a source of carbon and nitrogen under anaerobic conditions. The above findings from this research not only significantly advance understanding of A-PAM's environmental behavior but also contribute to the effective management practices in oil sands tailings.

Comprehensive characterization of organics in oil sands process water in constructed mesocosms utilizing multiple analytical methods.

This study aims to provide a thorough characterization of dissolved organics in oil sands process water (OSPW) in field-based aquatic mesocosms at both molecular and bulk measurement levels using multiple analytical methods. In a 3-year outdoor mesocosm experiment, the analysis of naphthenic acid (NA) species was conducted using ultra-performance liquid chromatography time-of-flight mass spectrometry (UPLC-TOFMS). The results revealed the removal of both total NAs (38% and 35%) and classical NAs (O2-NAs, 58% and 49%) in undiluted and half-diluted OSPW, respectively. The increased ratios of oxidized NAs (O3-O6 NAs) to classical NAs suggested a transformation trend. The results also indicated that O2-NAs with higher carbon number and lower double bond equivalent (DBE) were more easily degraded in the mesocosm systems. Biomimetic extraction using solid-phase microextraction (BE-SPME) measurement displayed 26% (undiluted OSPW) and 30% (half-diluted OSPW) decrease in total bioavailable organics over 3 years. Naphthenic acids fraction compounds (NAFCs) obtained by liquid-liquid extraction (LLE) were also determined using Fourier transform infrared spectroscopy (FTIR). Reduction in acute toxicity for undiluted (43%) and half-diluted (26%) OSPW was observed over 3 years, which are well correlated with the decreases of NAs and BE-SPME concentrations. Moreover, BE-SPME values were found to be linearly correlated with total NAs concentrations (r = 0.96) and NAFCs (r = 0.96). Additionally, the linear relationships of individual O2-O6 NA species and BE-SPME concentrations unveiled the changes in the relative abundances of O2-O6 NA species in total bioavailable organics over time in the mesocosms. The present study has provided comprehensive insights by integrating various analytical methods, contributing valuable information for assessing the effectiveness of aquatic mesocosm systems in studying the temporal changes of organics in OSPW.

Exploring nature's filters: Peat-mineral mix for low and high-strength oilfield produced water reclamation.

Nature-based solutions are encouraged for treating oilfield produced water from oil and gas extraction, a crucial undertaking that aligns with the Canadian oil sands industry's ambitious goal of zero waste, and the globally recognized Sustainable Development Goals (SDGs) pertaining to water conservation and ecosystem preservation. This study explored the use of peat-mineral mix (PMM), a leftover of inevitable oil sands mining, for treating low and high-strength wastewaters during biofiltration, which contained large molecular weight (44.3 kDa), which include alcohols, aliphatics, aromatics, and ketones, and can impart high toxicity to both fauna and flora (MicroTox: 99 %). The breakthrough curve indicated an effective initial adsorption phase driven by advection within the column dynamics. For complete organics removal and mechanistic insights, the wastewater was re-circulated in a continuous mode for up to 42 days. Here, we found that chemical oxygen demand was reduced from ∼85,000 mg/L to ∼965 mg/L). Kinetics investigations along with physicochemical characterization of PMM and wastewater suggested that chemisorption and anaerobic digestion contributed to the overall removal of contaminants. Chemisorption, led by hydrogen bonding and hydrophobic interactions, was the dominant mechanism, with a limited contribution from physical adsorption (surface area: 2.85 m2/g). The microbial community within the PMM bed was rich/diverse (Shannon > 6.0; Chao1 > 600), with ∼ 50 % unclassified phylotypes representing 'microbial dark matter'. High electric conductivity (332.1 µS cm-1) of PMM and the presence of Geobacter, syntrophs, and Methanosaeta suggest that direct interspecies electron transfer was likely occurring during anaerobic digestion. Both low and high-strength wastewaters showed effective removal of dissolved organics (e.g., naphthenic acids, acid extractable fraction, oil and grease content), nutrients, and potentially toxic metals. The successful use of PMM in treating oilfield produced water offers promising avenues for embracing nature-based remediation solutions at oil refining sites.

Experimental and theoretical insight into carbamazepine degradation by chlorine-based advanced oxidation processes: Efficiency, energy consumption, mechanism and DBPs formation.

Chlorine has been widely used in different advanced oxidation processes (AOPs) for micropollutants removal. In this study, different chlorine-based AOPs, namely medium pressure (MP) UV/chlorine, low pressure (LP) UV/chlorine, and in-situ chlorination, were compared for carbamazepine (CBZ) removal efficiency, energy consumption, and disinfection by-products (DBPs) formation. All three processes could achieve nearly 100% CBZ removal, while the reaction time needed by in-situ chlorination was double the time required by UV/chlorine processes. The energy consumed per magnitude of CBZ removed (EE/O) of MP UV/chlorine was 13 times higher than that of LP UV/chlorine, and relative to that of in-situ chlorination process. Accordingly, MP and LP UV/chlorine processes generated one to two orders of magnitude more hydroxyl radicals (•OH) and reactive chlorine species (RCS) than in-situ chlorination. Besides, RCS were the dominant reactive species, contributing to 78.3%, 75.6%, and 71.6% of CBZ removal in MP, LP UV/chlorine, and in-situ chlorination, respectively. According to the Gibbs free energy barriers between CBZ and RCS/•OH calculated based on density functional theory (DFT), RCS had more reaction routes with CBZ and showed lower energy barrier in the main CBZ degradation pathways like epoxidation and formation of iminostilbene. When applied to secondary wastewater effluent, UV/chlorine and in-situ chlorination produced overall DBPs ranging from 104.77 to 135.41 µg/L. However, the production of chlorate during UV/chlorine processes was 15 times higher than that during in-situ chlorination.

Examining the immunotoxicity of oil sands process affected waters using a human macrophage cell line.

Oil sands process affected water (OSPW) is produced during the surface mining of the oil sands bitumen deposits in Northern Alberta. OSPW contains variable quantities of organic and inorganic components causing toxic effects on living organisms. Advanced Oxidation Processes (AOPs) are widely used to degrade toxic organic components from OSPW including naphthenic acids (NAs). However, there is no established biological procedure to assess the effectiveness of the remediation processes. Our previous study showed that human macrophage cells (THP-1) can be used as a bioindicator system to evaluate the effectiveness of OSPW treatments through examining the proinflammatory gene transcription levels. In the present study, we investigated the immunotoxicological changes in THP-1 cells following exposure to untreated and AOP-treated OSPW. Specifically, using proinflammatory cytokine protein secretion assays we showed that AOP treatment significantly abrogates the ability of OSPW to induce the secretion of IL-1ß, IL-6, IL-8, TNF-α, IL-1Ra and MCP-1. By measuring transcriptional activity as well as surface protein expression levels, we also showed that two select immune cell surface markers, CD40 and CD54, were significantly elevated following OSPW exposure. However, AOP treatments abolished the immunostimulatory properties of OSPW to enhance the surface expression of these immune proteins. Finally, a transcriptome-based approach was used to examine the proinflammatory effects of OSPW as well as the abrogation of immunotoxicity following AOP treatments. Overall, this research shows how a human macrophage cell-based biomonitoring system serves as an effective in vitro tool to study the immunotoxicity of OSPW samples before and after targeted remediation strategies.

A novel biochar composite derived from oil-based drill sludge and cuttings: Structural characterization and electrochemical properties.

How to dispose of large quantities of hazardous shale gas drilling waste is an important worldwide problem facing the oil and gas industry. In this study, we report an environmentally friendly and low energy consumption approach (carbonization followed by activation) to convert oil-based drill sludge (OBDS) and oil-based drill cuttings (OBDCs) into biochar composites and investigate the effect of hydrofluoric acid (HF) acidification on them. The biochar composites were prepared using the OBDS, OBDCs, the mixtures of OBDS and OBDCs, and HF treatment the mixtures were named OS, OC, OSC, and OSC-HF, respectively. The characterization result of synthesized biochar composites indicated that the OSC had a larger specific surface area and a higher degree of graphitization. The composites mainly consisted of SiO2 and BaSO4, except for biochar. The OSC electrode exhibited the highest oxygen evolution potential (1.72 V vs Ag/AgCl) and the lowest charge transfer resistance compared with OS, OC, and OSC-HF electrodes, implying that SiO2 plays an important role in electrochemical performance. Using the OSC electrode as an anode, the chemical oxygen demand removal efficiency of the OBDS supernatant was 79.4 ± 0.95%. Further, the OSC electrode could maintain higher degradation efficiency and stability after the fifth reuse. The study provides a promising route for the proper disposal and resource utilization of OBDS and OBDCs and proposes a novel biochar compound as an electrode for the efficient treatment of wastewater. Moreover, this work highlights the important significance of the simultaneous resource utilization of waste and the treatment of wastewater using waste materials.

Role of pretreatment type and microbial mechanisms on enhancing volatile fatty acids production during anaerobic fermentation of refinery waste activated sludge.

This study compared the effects of alkaline, thermal, thermal-peroxymonosulfate (PMS), and alkyl polyglucose (APG) pretreatments on volatile fatty acids (VFAs) production from refinery waste activated sludge (RWAS), including VFAs yield, composition, organics components, microbial communities, and the potential improvement of mechanisms. All pretreatments effectively enhanced the bioconversion of RWAS and consequently promoted the hydrolysis process, which inhibited the methanogenesis process. However, the release of lignin/carboxyl-rich alicyclic molecules (CRAM)-like compounds and tannin substances in Thermal-PMS and APG groups significantly influenced the acidogenesis and acetogenesis processes. Among all pretreatments, alkaline pretreatment showed the highest VFAs yield of 95.06 mg/g volatile solids (VS) and VS removal of 17%. This result could be associated with the enrichment of functional hydrolytic-acidification bacteria, such as Planococcus and Soehngenia, and increased metabolism of amino acids, carbohydrates, and nucleotides. By considering an economical and efficient perspective, this study recommended the alkaline pretreatment for the anaerobic fermentation of RWAS.

Z-scheme plasmonic Ag decorated Bi2WO6/NiO hybrids for enhanced photocatalytic treatment of naphthenic acids in real oil sands process water under simulated solar irradiation.

A novel photocatalyst, Bi2WO6/NiO/Ag, with hierarchical flower-like Z-scheme heterojunction, which exhibited excellent stability and photocatalytic activity over a wide light spectrum, was firstly synthesized and used in the remediation of real oil sands process water (OSPW) and achieved complete removal of aromatics, classical naphthenic acids (NAs), and heteroatomic NAs after 6 h of photocatalytic treatment. The acute toxicity of OSPW was completely eliminated after only 2 h of treatment. h+ and ∙OH were found to be the major oxidative species in the photocatalytic system. The enhanced photocatalytic efficiency is the result of the unique Z-scheme electron transfer among electron mediators Ag, NiO, and Bi2WO6, which was supported by the in-situ irradiated XPS. The study benefits the design of engineered passive treatment approach for OSPW remediation through solar light-driven catalyst.

Performance of constructed floating wetlands in a cold climate waste stabilization pond.

Effectiveness of constructed floating wetlands (CFWs) is largely unknown for wastewater treatment in cold climates. An operational-scale CFW system was retrofitted into a municipal waste stabilization pond in Alberta, Canada. During the first year (Study I), insignificant performance was recorded for water quality parameters, although phyto-uptake of elements was evident. In Study II, doubling of the CFW area and addition of underneath aeration promoted plant uptake of elements, including nutrients and metals, following significant pollutant reduction in the water; 83 % of chemical oxygen demand, 80 % of carbonaceous biochemical oxygen demand, 67 % of total suspended solids, and 48 % of total Kjeldhal nitrogen. A mesocosm study, conducted in parallel to the pilot scale field study, confirmed the impact of both vegetation and aeration on water quality improvement. The phytoremediation potential was linked to accumulation within plant shoot and root biomass and was confirmed by mass balance. Bacterial community analyses reflected that heterotrophic nitrification, aerobic denitrification, complete denitrification, organic matter decomposition, and methylotrophy were dominant mechanisms in the CFW, likely resulting in successful transformation of organics and nutrients. CFWs appear to be a viable ecotechnology to treat municipal wastewater in Alberta; however, larger and aerated CFW systems are recommended to achieve maximum remediation. The study aligns with the United Nations Environment Program to scale up restoration of degraded ecosystems, and to improve conditions for water supply and biodiversity following recognition of 2021-2030 as the Decade on Ecosystem Restoration.

Sorption and desorption of naphthenic acids on reclamation materials: Mechanisms and selectivity of naphthenic acids from oil sands process water.

This study investigated the application of materials peat-mineral mix (PT) and Pleistocene fluvial sands from different location (PF-1 and PF-2) obtained from surface mining of oil sands as sorbents of naphthenic acids (NAs) from oil sands process water (OSPW). To understand the sorption properties and mechanisms of NAs in the materials, sorption and desorption studies were performed using decanoic acid (DA) and 5-phenylvaleric acid (PVA). Additionally, the removal efficiency was evaluated using real OSPW to understand the effect of NA structure on sorption. Equilibrium of DA and PVA was reached at 2 days for PT, and 3 and 6 days for PF materials, respectively. Langmuir isotherm best fitted the equilibrium data. Maximum sorption capacities for DA and PVA were, respectively, 16.8 × 103 and 104 mg/kg for PT, 142.9 and 81.3 mg/kg for PF-1, and 600 and 476.2 mg/kg for PF-2. Hydrophobic interactions, hydrogen bonding, and π-π interaction were the main sorption mechanisms. Desorption of model compounds from post-sorption materials was not observed for 14 days. The removal of NAs from real OSPW ranged from 20 to 54%. PT is the most promising sorbent of NAs from OSPW because it partially removed NAs with a wide range of molecular weights and structures at very low dosage. Sorption of NAs was affected by the total organic carbon of the materials, emphasizing the hydrophobic interaction as an important sorption mechanism. The results suggest that some mobility of NAs is expected to take place if the reclamation materials come in contact with OSPW, which might occur in an oil sands reclamation landscape.

Aerobic degradation of anionic polyacrylamide in oil sands tailings: Impact factor, degradation effect, and mechanism.

An investigation was carried out to study the degradation of anionic polyacrylamide (A-PAM) under different temperature and microorganism conditions as well as to assess its effects on water chemistry and toxicity in oil sands tailings. The maximum removal efficiency of A-PAM was 41.0 % in tailings water with augmented microorganisms at 20 °C. No acrylamide (AMD) monomer was released during the A-PAM degradation, while residual AMD, from the manufacturing process to make A-PAM, was completely removed within 4 weeks. Both temperature and microorganisms showed significant effects (p < 0.05) on the degradation of A-PAM and residual AMD. Gel permeation chromatography (GPC) and Fourier transform infrared (FT-IR) analyses showed that biodegradation could be the active pathway for A-PAM degradation in oil sands tailings. These analyses also indicated that macromolecular A-PAM was degraded into lower molecular weight organic compounds. No remarkable changes of the total concentration of naphthenic acids (NAs) were observed in A-PAM treated tailings water. However, low concentrations of fatty acids (<2.5 mg/L), which fit the NAs formula, were detected in pure polymer solution, indicating that A-PAM degradation would not affect the total concentration of NAs in tailings water but affect their distribution. Our results also showed that total organic carbon (TOC) and chemical oxygen demand (COD) could be used as indicators of A-PAM degradation in tailings water due to their strong linear correlations (R2 > 0.90). Only slight increases in zeta potential and pH were found during A-PAM degradation. Limited effect on acute toxicity and no genotoxicity were found in A-PAM treated tailings water. Furthermore, the results suggest that A-PAM undergoes hydrolysis of amide groups by amidase enzymes, releasing ammonia and smaller molecules like organic acids. This research provides valuable information regarding the stability and impacts of A-PAM and thus will be beneficial for the management of oil sands tailings in long period of time.

Alkaline fermentation of refinery waste activated sludge mediated by refinery spent caustic for volatile fatty acids production.

Volatile fatty acids (VFA), produced from waste activated sludge (WAS), provide unique opportunities for resource recovery in wastewater treatment plants. This study investigates the potential of refinery spent caustic (RSC) on VFA production during refinery WAS (RWAS) alkaline fermentation. The highest VFA yield was 196.3 mg/g-VS at a sludge retention time of 6 days. Amplicon sequencing revealed the enrichment of Soehngenia (20.21%), Bacilli (11.86%), and Brassicibacter (4.17%), which was associated with improved activities of protease (626%) and α-glucosidase (715%). Function prediction analysis confirmed that acetyl-CoA production and fatty acid biosynthesis were enhanced, while fatty acid degradation was inhibited. Accordingly, hydrolysis, acidogenesis, and acetogenesis were improved by 6.87%, 10.67%, and 28.50%, respectively; whereas methanogenesis was inhibited by 28.87%. The sulfate and free ammonia in RSC likely contributed to increased acetic acid production. This study showcases that RWAS alkaline fermentation mediated by RSC for VFA production is the practicable approach.

CEG-AgNPs Ameliorates DMBA-Induced Mammary Carcinogenicity by Alleviating Cytokines Expression.

<b>Background and Objective:</b> For more than a decade, breast cancer has been one of the most common forms of cancer among women around the world. The present article aimed to evaluate the protective activity of CEG-AgNPs against DMBA-induced mammary carcinoma. <b>Materials and Methods:</b> In this experimental study, green synthesis and characterization of CEG-AgNPs were carried as well as IC₅₀ against Mcf7 cell line and LD₅₀ on mice were evaluated. A total of 24 adult albino mice were divided into four groups six rats in each. Group I was given an equal amount of distilled water, group II was received 80 mg kg¹ b.wt., DMBA for 4 weeks, groups III and IV were treated with CEG-AgNPs (28.1 and 70.25 mg kg¹) from the 5th week of DMBA administration for 4 weeks, respectively. <b>Results:</b> CEG-AgNPs were approximately 42.32±9.52 nm with a negative zeta potential of -17.44. It is IC₅₀ against the Mcf7 cell line and LD₅₀ is equal to 82.76 µg mL¹ and 1405 mg kg¹ b.wt., A significant normalization in plasma ALT, AST, AST and LDH as well as mammary MDA, TNF-α, IL-6, P53, SOD, GPx and GSH levels have been observed in CEG-AgNPs treated mice. Oral CEG-AgNPs administration has suppressed VEGF-C gene expression in DMBA-treated mice. <b>Conclusion:</b> The present results, biochemical, histological and MRI results showed that CEG-AgNPs have potent anticancer activity against DMBA-induced mammary carcinoma in mice by inducing the biosynthesizes of antioxidant biomarkers and suppression of cytokines gene expression.

Mixotrophic denitrification processes in basalt fiber bio-carriers drive effective treatment of low carbon/nitrogen lithium slurry wastewater.

Lithium battery slurry wastewater was successfully treatedby using basalt fiber (BF) bio-carriers in a biological contact oxidation reactor. This resulted in a significant reduction of COD (93.3 ± 0.5 %) and total nitrogen (77.4 ± 1.0 %) at 12 h of HRT and dissolved oxygen (DO) of 0-1 mg/L. The modified Stover-Kincannon model indicated that the total nitrogen removal rate was 4.462 kg/m3/d in R-BF while the substrate maximum specific reaction rate (qmax) in the Monod model was 0.323 mg-N/mgVSS/d. A stable internal environment was established within the bio-nest. Metataxonomic analysis revealed the presence of denitrification and decarbonization bacteria, combined heterotrophic nitrification-aerobic denitrification bacteria, nitrite-oxidizing bacteria, and ammonia-oxidizing bacteria. Functional analysis displayed changes related to (aerobic)chemoheterotrophy, nitrogen respiration, nitrate reduction, respiration/denitrification of nitrite, and nitrate in R-BF. The study proposes a novel approach to achieve denitrification for the treatment of lithium slurry wastewater at low C/N conditions.

Nitrogen removal intensification of aerobic granular sludge through bioaugmentation with "heterotrophic nitrification-aerobic denitrification" consortium during petroleum wastewater treatment.

The bioaugmentation potential of aerobic granular sludge (AGS) was investigated using heterotrophic nitrification-aerobic denitrification (HN-AD) bacterial consortium to improve nitrogen removal during petroleum wastewater treatment. An efficient HN-AD consortium was constructed by mixing Pseudomonas mendocina K0, Brucella sp. K1, Pseudomonas putida T4 and Paracoccus sp. T9. AGS bioaugmented by immobilized HN-AD consortium enhanced nitrogen removal, which showed NH4+-N and TN removal efficiency of 92.4% and 79.8%, respectively. The immobilized consortium addition facilitated larger AGS formation, while granules > 2.0 mm accounted for 16.7% higher than that of control (6.7%). Further, the abundance of napA gene was 4-times higher in the bioaugmented AGS as compared to the control, which demonstrated the long-term stability of HN-AD consortium in the bioreactor. The bioaugmented AGS also showed a higher abundance of xenobiotics biodegradation and nitrogen metabolism. These results highlight that bioaugmentation of AGS technology could be effectively used for enhanced denitrification of petroleum wastewater.

Review on carbon-based adsorbents from organic feedstocks for removal of organic contaminants from oil and gas industry process water: Production, adsorption performance and research gaps.

Large amounts of process water with considerable concentrations of recalcitrant organic contaminants, such as polycyclic aromatic hydrocarbon (PAHs), phenolic compounds (PCs), and benzene, toluene, ethylbenzene, and xylene (BTEX), are generated by several segments of oil and gas industries. These segments include refineries, hydraulic fracturing (HF), and produced waters from the extraction of shale gas (SGPW), coalbed methane (CBMPW) and oil sands (OSPW). In fact, the concentration of PCs and PAHs in process water from refinery can reach 855 and 742 mg L-1, respectively. SGPW can contain BTEX at concentrations as high as 778 mg L-1. Adsorption can effectively target those organic compounds for the remediation of the process water by applying carbon-based adsorbents generated from organic feedstocks. Such organic feedstocks usually come from organic waste materials that would otherwise be conventionally disposed of. The objective of this review paper is to cover the scientific progress in the studies of carbon-based adsorbents from organic feedstocks that were successfully applied for the removal of organic contaminants PAHs, PCs, and BTEX. The contributions of this review paper include the important aspects of (i) production and characterization of carbon-based adsorbents to enhance the efficiency of organic contaminant adsorption, (ii) adsorption properties and mechanisms associated with the engineered adsorbent and expected for certain pollutants, and (iii) research gaps in the field, which could be a guidance for future studies. In terms of production and characterization of materials, standalone pyrolysis or hybrid procedures (pyrolysis associated with chemical activation methods) are the most applied techniques, yielding high surface area and other surface properties that are crucial to the adsorption of organic contaminants. The adsorption of organic compounds on carbonaceous materials performed well at wide range of pH and temperatures and this is desirable considering the pH of process waters. The mechanisms are frequently pore filling, hydrogen bonding, π-π, hydrophobic and electrostatic interactions, and same precursor material can present more than one adsorption mechanism, which can be beneficial to target more than one organic contaminant. Research gaps include the evaluation of engineered adsorbents in terms of competitive adsorption, application of adsorbents in oil and gas industry process water, adsorbent regeneration and reuse studies, and pilot or full-scale applications.

Recent advances and future perspective on nanocellulose-based materials in diverse water treatment applications.

Over the past few years, nanocellulose and its derivatives have drawn attention as promising bio-based materials for water treatment applications due to their high surface area, high strength, and renewable, biocompatible nature. The abundance of hydroxyl functional groups on the surfaces of cellulose nanocrystals (CNCs) and cellulose nanofibrils (CNFs) enables a broad range of surface modifications which results in propitious nanocomposites with tunable characteristics. In this context, this review describes the continuously developing applications of nanocellulose-based materials in the areas of adsorption, catalysis, filtration, and flocculation, with a special emphasis on the removal of contaminants such as heavy metals, dyes, and pharmaceutical compounds from diverse water systems. Recent progresses in the diverse forms of application of nanocellulose adsorbents (suspension, hydrogel, aerogel, and membrane) are also highlighted. Finally, challenges and future perspectives on emerging nanocellulose-based materials and their possible industrial applications are presented and discussed.

Low carbon-to-nitrogen ratio digestate from high-rate anaerobic baffled reactor facilitates heterotrophic/autotrophic nitrifiers involved in nitrogen removal.

In this study, baffled anaerobic-aerobic reactors (AOBRs) with modified basalt fiber (MBF) carriers and felt were used to treat domestic wastewater (DWW). The influent was first treated in anaerobic compartments, with the NH4+-N containing digestate refluxed into aerobic compartment for nitrification. The nitrified liquid was channeled to the anaerobic compartments for further denitrification. Under optimal conditions, AOBR with MBF carriers could remove 91% chemical oxygen demand (COD) and 81% total nitrogen (TN), with biomass production increased by 7.6%, 4.5% and 8.7% in three successive anaerobic compartments compared to the control. Biological viability analysis showed that live cells outnumbered dead cells in bio-nests. Metagenomics analysis showed that multiple metabolic pathways accounted for nitrogen conversion in anaerobic and aerobic compartments. More importantly, low COD/TN ratio digestate facilitated heterotrophic nitrification-aerobic denitrification (HN-AD) species growth in aerobic compartment. This study provides a promising strategy to source treatment of DWW from urban communities.

O3/H2O2 and UV-C light irradiation treatment of oil sands process water.

The oil sands industry generates large volumes of oil sands process water (OSPW). There is an urgent need for OSPW treatment to reduce process water inventories and to support current reclamation approaches. This study discusses how efficient ozone (O3)-based combined advanced oxidation processes (AOPs), including hydrogen peroxide (H2O2) and UV-C, are at achieving mineralization while reducing the toxicity arising from such organic components as naphthenic acids (NAs) in OSPW. The results showed that the dissolved organic carbon (DOC) removals of 45%, 84%, 84% and 98%, obtained after 90-min treatments with O3, O3/H2O2, UVC/O3 and UVC/O3/H2O2, respectively, at a production rate of 6 g/L·h O3 were considerably higher than at lower O3 production rates. The acute toxicity on Vibrio fischeri was significantly reduced by all the treatments, which explains the high percentages of NA removal (up to 99% as confirmed by UPLC-QTOF-HRMS.) Mineralization (expressed as DOC removal) was highest with UVC/O3/H2O2 at ca. 2 mg C/L in the treated effluent, which means that it could be used as cooling/boiling process water in bitumen upgrading units. However, considering the energy demand of the treatments tested, the treatment using O3/H2O2 was found to be the most realistic for large-scale applications.

Decomplexation of Cu(II)-EDTA by synergistic activation of persulfate with alkali and CuO: Kinetics and activation mechanism.

Heavy metals usually coexist with a variety of chelating agents to form heavy metal complexes in industrial wastewater. The decomplexation of heavy metal complexes is the crucial step before the removal of heavy metals via alkaline precipitation process. An efficient synergistic activation of persulfate (PS) with alkali and CuO was used for the simultaneous decomplexation of Cu-ethylenediamine tetraacetic acid (Cu(II)-EDTA) (3.14 mM) and the Cu(II) precipitation. The experimental results demonstrated that nearly complete removal of Cu(II) could be achieved by synergistic activation of PS with alkali and CuO at pH 11 after 2 h of decomplexation reaction. However, sole PS could not effectively decomplex Cu(II)-EDTA (13.5%), while the alkaline activation of PS could accomplish 57.0% removal of Cu(II). Radical scavenger tests indicated that reactive oxygen species (ROS) including SO4•-, •OH and O2•- were responsible for the decomplexation of Cu(II)-EDTA in the synergistic activation of PS with alkali and CuO. As a heterogeneous activator, CuO possessed excellent reusability and long-lasting catalytic activity and the rate constant value (k) of Cu(II) removal showed an increase (from 0.0326 min-1 in the first cycle to 0.0491 min-1 in the 24th cycle) with 24 cycles experiments. Furthermore, the biotoxicity evaluation of treated solution revealed that the biotoxicity of Cu(II)-EDTA contaminated wastewater could be effectively mitigated by the synergistic activation of PS with alkali and CuO because of the efficient precipitation of Cu(II) and oxidative degradation of EDTA organic ligands, which was favorable for the subsequent biochemical treatment.