RESUMO
Antibodies against Spotted Fever Group (SFG) Rickettsia and Coxiella burnetii, investigated through indirect antibody immunofluorescence tests, were detected in serum samples from 3.1% and 0% of 358 rural dogs, respectively, and in none of 32 wild foxes tested. SFG Rickettsia seropositive dogs were only detected in the Mountain Desert (8%) and the Steppe-Mediterranean (9%) regions. Exposure in the Mountain Desert, where no ticks and fleas were found on any dog, could correspond to a new SFG Rickettsia sp. recently described in soft ticks or to a related agent. Our survey confirms low endemicity in the country of C. burnetii, as observed in recent serosurveys in humans.
Assuntos
Coxiella burnetii , Doenças do Cão , Rickettsia , Rickettsiose do Grupo da Febre Maculosa , Animais , Chile/epidemiologia , Doenças do Cão/microbiologia , Cães , Raposas , Rickettsiose do Grupo da Febre Maculosa/epidemiologia , Rickettsiose do Grupo da Febre Maculosa/veterináriaRESUMO
Medicinal Inorganic Chemistry offers the high diversity of metal coordination chemistry for the development of bioactive compounds for therapeutic or diagnostic medicinal purposes. The design of novel metal-based antitumor agents occupies a privileged position in this discovery process. On the other hand, the research on metal-based radiopharmaceuticals for therapy and imaging is a subarea of high priority and development. This review describes therapeutic applications of metal compounds directed towards hypoxic tissues. Strategies in the search for new bioreductive metal-based prodrugs will be discussed. In addition, approaches for the imaging of hypoxic tissues by using metal radionuclides will be exemplified.
Assuntos
Antineoplásicos/uso terapêutico , Hipóxia Celular , Compostos Organometálicos/uso terapêutico , Radioisótopos , Antineoplásicos/química , Antineoplásicos/metabolismo , Citotoxinas/química , Citotoxinas/metabolismo , Citotoxinas/uso terapêutico , Desenho de Fármacos , Humanos , Metais/química , Compostos Organometálicos/química , Radioisótopos/uso terapêutico , Compostos Radiofarmacêuticos/uso terapêuticoRESUMO
The potassium salt of the carbonato oxodiperoxovanadate(V) complex, obtained by a known synthetic procedure, was thoroughly characterized by infrared, Raman, and electronic spectroscopy. The bioactivity of the complex on the cell proliferation was tested on osteoblast-like cells (MC3T3E1 osteoblastic mouse calvaria-derived cells and UMR106 rat osteosarcoma-derived cells) in culture. At low doses, the complex is more toxic for the nontransformed osteoblasts than for the tumoral ones, whereas at higher doses the deleterious effects are similar for both cell lines. This peroxo complex seems to be the most toxic compound that has so far been tested on osteoblast-like cells in culture.
Assuntos
Compostos de Vanádio/farmacologia , Animais , Linhagem Celular , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Camundongos , Ratos , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
In order to get insight into the trypanocidal mechanism of action of a series of 5-nitrofuryl containing thiosemicarbazones some studies related to their bioreduction were performed. Electron spin resonance spectra of radicals generated in T. cruzi by compounds' bioreduction were analyzed. Three different patterns of ESR signals were observed for the different assayed compounds. These results were in agreement with the changes in the T. cruzi-oxygen uptake promoted by these compounds. On the other hand, free-radical scavenger properties, measured as oxygen radical absorbance capacity (ORAC), did not seem to correlate with the trypanocidal activity.