Fungal skin for robots.

Advancements in mycelium technology, stemming from fungal electronics and the development of living mycelium composites and skins, have opened new avenues in the fusion of biological and artificial systems. This paper explores an experimental endeavour that successfully incorporates living, self-regenerating, and reactive Ganoderma sessile mycelium into a model cyborg figure, creating a bio-cybernetic entity. The mycelium, cultivated using established techniques, was homogeneously grown on the cyborg model's surface, demonstrating robust reactivity to various stimuli such as light exposure and touch. This innovative merger points towards the future of sustainable biomaterials and the potential integration of these materials into new and existing technologies.

Impact of malt concentration in solid substrate on mycelial growth and network connectivity in Ganoderma species.

With its distinctive material properties, fungal mycelium has emerged as an innovative material with a diverse array of applications across various industries. This study focuses on how the growth strategies of wood fungi adapt to nutrient availability. The effect of malt extract concentration in the growth medium on radial growth kinetics, morphology, mycelium network connectivity, and mechanical characteristics of mycelium from two Ganoderma species were investigated. While an evident pattern of radial growth rate enhancement with malt concentrations was not apparent, there was a discernible trend towards denser mycelium network characteristics as revealed by spectrophotometry. Increased malt extract contents corresponded to elevated optical density measurements and were visually confirmed by denser mycelium networks in photographic images. Investigating the mechanical characteristics of mycelium cultivated on varying solid substrate concentrations, the Young's modulus exhibited a substantial difference between mycelium grown on 5 wt% malt substrate and samples cultivated on 2 wt% and 0.4 wt% malt substrates. The obtained results represent a new understanding of how malt availability influences mycelial growth of two Ganoderma species, a crucial insight for potentially refining mycelium cultivation across diverse applications, including meat alternatives, smart building materials, and alternative leather.

Electrical response of fungi to changing moisture content.

Mycelium-bound composites are potential alternatives to conventional materials for a variety of applications, including thermal and acoustic building panels and product packaging. If the reactions of live mycelium to environmental conditions and stimuli are taken into account, it is possible to create functioning fungal materials. Thus, active building components, sensory wearables, etc. might be created. This research describes the electrical sensitivity of fungus to changes in the moisture content of a mycelium-bound composite. Trains of electrical spikes initiate spontaneously in fresh mycelium-bound composites with a moisture content between [Formula: see text] 95% and [Formula: see text] 65%, and between [Formula: see text] 15% and [Formula: see text] 5% when partially dried. When the surfaces of mycelium-bound composites were partially or totally encased with an impermeable layer, increased electrical activity was observed. In fresh mycelium-bound composites, electrical spikes were seen both spontaneously and when induced by water droplets on the surface. Also explored is the link between electrical activity and electrode depth. Future designs of smart buildings, wearables, fungi-based sensors, and unconventional computer systems may benefit from fungi configurations and biofabrication flexibility.

Electrical spiking of psilocybin fungi.

Psilocybin fungi, aka "magic" mushrooms, are well known for inducing colorful and visionary states of mind. Such psychoactive properties and the ease of cultivating their basidiocarps within low-tech setups make psilocybin fungi promising pharmacological tools for mental health applications. Understanding of the intrinsic electrical patterns occurring during the mycelial growth can be utilized for better monitoring the physiological states and needs of these species. In this study we aimed to shed light on this matter by characterizing the extra-cellular electrical potential of two popular species of psilocybin fungi: Psilocybe tampanensis and P. cubensis. As in previous experiments with other common edible mushrooms, the undisturbed fungi have shown to generate electric potential spikes and trains of spiking activity. This short analysis provides a proof of intrinsic electrical communication in psilocybin fungi, and further establishes these fungi as a valuable tool for studying fungal electro-physiology.

Fungal electronics.

Fungal electronics is a family of living electronic devices made of mycelium bound composites or pure mycelium. Fungal electronic devices are capable of changing their impedance and generating spikes of electrical potential in response to external control parameters. Fungal electronics can be embedded into fungal materials and wearables or used as stand alone sensing and computing devices.

Fungi anaesthesia.

Electrical activity of fungus Pleurotus ostreatus is characterised by slow (h) irregular waves of baseline potential drift and fast (min) action potential likes spikes of the electrical potential. An exposure of the myceliated substrate to a chloroform vapour lead to several fold decrease of the baseline potential waves and increase of their duration. The chloroform vapour also causes either complete cessation of spiking activity or substantial reduction of the spiking frequency. Removal of the chloroform vapour from the growth containers leads to a gradual restoration of the mycelium electrical activity.

Stimulating Fungi Pleurotus ostreatus with Hydrocortisone.

Fungi cells can sense extracellular signals via reception, transduction, and response mechanisms, allowing them to communicate with their host and adapt to their environment. They feature effective regulatory protein expressions that enhance and regulate their response and adaptation to various triggers such as stress, hormones, physical stimuli such as light, and host factors. In our recent studies, we have shown that Pleurotus oyster fungi generate electrical potential impulses in the form of spike events in response to their exposure to environmental, mechanical, and chemical triggers, suggesting that the nature of stimuli may be deduced from the fungal electrical responses. In this study, we explored the communication protocols of fungi as reporters of human chemical secretions such as hormones, addressing whether fungi can sense human signals. We exposed Pleurotus oyster fungi to hydrocortisone, which was directly applied to the surface of a fungal-colonized hemp shavings substrate, and recorded the electrical activity of the fungi. Hydrocortisone is a medicinal hormone replacement that is similar to the natural stress hormone cortisol. Changes in cortisol levels released by the body indicate the presence of disease and can have a detrimental effect on physiological process regulation. The response of fungi to hydrocortisone was also explored further using X-rays to reveal changes in the fungi tissue, where receiving hydrocortisone by the substrate can inhibit the flow of calcium and, as a result, reduce its physiological changes. This research could open the way for future studies on adaptive fungal wearables capable of detecting human physiological states and biosensors built of living fungi.

Towards fungal sensing skin.

A fungal skin is a thin flexible sheet of a living homogeneous mycelium made by a filamentous fungus. The skin could be used in future living architectures of adaptive buildings and as a sensing living skin for soft self-growing/adaptive robots. In experimental laboratory studies we demonstrate that the fungal skin is capable for recognising mechanical and optical stimulation. The skin reacts differently to loading of a weight, removal of the weight, and switching illumination on and off. These are the first experimental evidences that fungal materials can be used not only as mechanical 'skeletons' in architecture and robotics but also as intelligent skins capable for recognition of external stimuli and sensorial fusion.

Flexible Fungal Materials: Shaping the Future.

Fungi are a revolutionary, smart, and sustainable manufacturing platform that can be used to upcycle byproducts and wastes into flexible fungal materials (FFMs) such as chitin- and ß-glucan-based foams, paper, and textiles. With highly adaptable manufacturing pathways, the efficiency and properties of these materials depend on the biomass source and fermentation method. Liquid substrates provide fast, upscalable, and compact production processes but are susceptible to contamination and are limited to paper-like materials for printing, wound dressings, and membranes. Solid-state fermentation is cheaper but struggles to deliver homogeneous fungal growth and is used to produce fungal foams for packaging, insulation, textiles, and leather substitutes. The broad range of applications and uses of biological organisms in materials hallmarks fungi as forerunners in improving environmental sustainability globally.

Fungal sensing skin.

BACKGROUND: A fungal skin is a thin flexible sheet of a living homogeneous mycelium made by a filamentous fungus. The skin could be used in future living architectures of adaptive buildings and as a sensing living skin for soft self-growing/adaptive robots. RESULTS: In experimental laboratory studies we demonstrate that the fungal skin is capable for recognising mechanical and optical stimulation. The skin reacts differently to loading of a weight, removal of the weight, and switching illumination on and off. CONCLUSION: These are the first experimental evidences that fungal materials can be used not only as mechanical 'skeletons' in architecture and robotics but also as intelligent skins capable for recognition of external stimuli and sensorial fusion.

Reactive fungal wearable.

Smart wearables sense and process information from the user's body and environment and report results of their analysis as electrical signals. Conventional electronic sensors and controllers are commonly, sometimes augmented by recent advances in soft electronics. Organic electronics and bioelectronics, especially with living substrates, offer a great opportunity to incorporate parallel sensing and information processing capabilities of natural systems into future and emerging wearables. Nowadays fungi are emerging as a promising candidate to produce sustainable textiles to be used as ecofriendly biowearables. To assess the sensing potential of fungal wearables we undertook laboratory experiments on electrical response of a hemp fabric colonised by oyster fungi Pleurotus ostreatus to mechanical stretching and stimulation with attractants and repellents. We have shown that it is possible to discern a nature of stimuli from the fungi electrical responses. The results paved a way towards future design of intelligent sensing patches to be used in reactive fungal wearables.

A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins.

Antivenoms developed from the plasma of hyperimmunized animals are the only effective treatment available against snakebite envenomation but shortage of supply contributes to the high morbidity and mortality toll of this tropical disease. We describe a synthetic biology approach to affordable and cost-effective antivenom production based on plant-made recombinant polyclonal antibodies (termed pluribodies). The strategy takes advantage of virus superinfection exclusion to induce the formation of somatic expression mosaics in agroinfiltrated plants, which enables the expression of complex antibody repertoires in a highly reproducible manner. Pluribodies developed using toxin-binding genetic information captured from peripheral blood lymphocytes of hyperimmunized camels recapitulated the overall binding activity of the immune response. Furthermore, an improved plant-made antivenom (plantivenom) was formulated using an in vitro selected pluribody against Bothrops asper snake venom toxins and has been shown to neutralize a wide range of toxin activities and provide protection against lethal venom doses in mice.

A coat-independent superinfection exclusion rapidly imposed in Nicotiana benthamiana cells by tobacco mosaic virus is not prevented by depletion of the movement protein.

New evidence is emerging which indicates that population variants in plant virus infections are not uniformly distributed along the plant, but structured in a mosaic-like pattern due to limitation to the superinfection imposed by resident viral clones. The mechanisms that prevent the infection of a challenge virus into a previously infected cell, a phenomenon known as superinfection exclusion (SE) or Homologous Interference, are only partially understood. By taking advantage of a deconstructed tobacco mosaic virus (TMV) system, where the capsid protein (CP) gene is replaced by fluorescent proteins, an exclusion mechanism independent of CP was unveiled. Time-course superinfection experiments provided insights into SE dynamics. Initial infection levels affecting less than 10 % of cells led to full immunization in only 48 h, and measurable immunization levels were detected as early as 6 h post-primary infection. Depletion of a functional movement protein (MP) was also seen to slow down, but not to prevent, the SE mechanism. These observations suggest a CP-independent mechanism based on competition for a host-limiting factor, which operates at very low virus concentration. The possible involvement of host factors in SE has interesting implications as it would enable the host to influence the process.