Radioprotective efficacy of dieckol against gamma radiation-induced cellular damage in hepatocyte cells.

Naturally occurring antioxidants prevent or delay the harmful effect of free radical formation and radioprotection. The present study aimed to investigate the radioprotective effect of dieckol, a naturally occurring marine bioactive phenolic compound on lipid peroxidation and antioxidant status, DNA damage, and inflammation in gamma-radiation-induced rat primary hepatocytes. Isolated hepatocyte cells exposed to gamma-radiation showed an increased level of lipid peroxidation markers (thiobarbituric acid reactive substances and lipid hydroperoxides) accompanied with the decrease in the activities of enzymatic (SOD, CAT, and GPx) and non-enzymatic (vitamin C, vitamin E, and GSH) antioxidants associated with increased DNA damage coupled with upregulation of inflammatory proteins (NF-κB and COX-2) compared to control. Treatment of dieckol (5, 10, 20 µM) reduces the γ-radiation-induced toxicity and the associated pro-oxidant and antioxidant imbalance as well as decreasing the DNA damage (tail length, tail moment, %DNA in a tail and olive tail moment) and inflammation in hepatocyte cells. These findings indicate that treatment of dieckol offers protection against γ-radiation-induced cellular damage in the liver cells.

Harmful metals concentration in sediments and fishes of biologically important estuary, Bay of Bengal.

Study on the accumulation level of heavy metals was conducted on sediment and fishes from estuaries of Bay of Bengal. Heavy metals were determined by using Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES) and the results were compared to permissible limits of WHO/USEPA. The accumulation patterns of Fe and Cd were found predominantly in all samples tested when correlated with other metals. It was found that the concentration of metals such as Cd (3.90 ± 0.25 µg/g), Cr (0.44 ± 0.05 µg/g), Ni (0.33 ± 0.01 µg/g), and Mn (1.1 ± 0.11 µg/g) were exceeding the permissible limit, whereas Fe, Co, Pb, and Zn were found within the limit of WHO/USEPA at station 1. In station 2, Cd (16.5 ± 0.4 µg/g), Mn (0.67 ± 0.11 µg/g), and Cr (0.80 ± 0.01 µg/g) were exceeding the permissible limit, whereas Fe, Co, Pb, Ni, and Zn were found within the limit. This study emphasizes that Cd and Mn levels in both stations, are far higher than the acceptable values set by WHO/USEPA and may therefore present human health hazards. It is therefore mandatory to carry out extensive research to evaluate the possible environmental risk factors in the vicinity of both estuaries with respect to heavy metals.

Determination of metal levels in thirteen fish species from Lakshadweep Sea.

A bio-monitoring study was performed to investigate the concentration of certain metals (cadmium, cobalt, copper, iron, magnesium, manganese, nickel, lead and zinc) in gill, skin and muscle of thirteen demersal fish species of Agatti Coast of Lakshadweep Sea. All the metal concentrations in gill are higher than skin and muscle. Concentrations of toxic metals such as Cd (0.61 ± 0.67 µg g(-1) in Gerres longirostris), Mn (0.83 ± 1.21 µg g(-1) in Lutjanus fulvus) and Ni (0.56 ± 0.83 µg g(-1) in L. bengalensis) were well above the permissible limits suggested by World Health Organization and Food and Agricultural Organizations.