Interdependence of drug dose, incubation time & light dose on hematoporphyrin derivative induced photoinactivation of brain tumour cells.

The relationship between photosensitizer concentration, light dose, incubation time and cellular damage in human cerebral glioma cells in culture, was studied. Cells were incubated with hematoporphyrin derivative (Hpd) for different durations at 37 degrees C. Immediately after specified period of incubation, cells were irradiated with white light. Cellular damage was assessed by colony forming ability of cells. A progressive reduction in the surviving fraction was observed as a function of drug and light dose. The survival curves were of exponential nature with an initial shoulder. The cell survival was found to be dependent on the time of incubation with Hpd. These results suggest that photodynamic cellular damage can be enhanced at low drug and light dose by increasing the incubation time.

Molecular characterization of an anion pump. The arsA gene product is an arsenite(antimonate)-stimulated ATPase.

The products of the arsenical resistance operon of resistance plasmid R733 form an efflux system for arsenicals. Detoxification results from active efflux of the oxyanions, preventing their concentration from reaching toxic levels. The largest polypeptide encoded by the ars operon was purified. From N-terminal sequencing the purified protein, termed the ArsA protein, was shown to correspond to the product of the arsA gene. The purified protein was demonstrated to bind ATP by two methods. First, a photoadduct of the protein with [alpha-32P]ATP was formed by irradiation at 254 nm. Second, the purified protein bound a fluorescent ATP analogue, 2',3'-o-(2,4,6)trinitrophenyl ATP, with a half-maximal affinity of 2 microM. By both assays competition was observed with ATP or ADP, but not with AMP, GTP, CTP, or UTP. In both nucleotide binding assays, Mg2+ was required, but neither arsenite nor antimonate had any affect. In contrast, the ArsA protein exhibited an ATPase activity which was dependent on the presence of arsenite or antimonate. The results suggest that the ArsA protein is the catalytic subunit of an oxyanion-translocating ATPase.

Maintenance of intracellular calcium in Escherichia coli.

Recently a series of fluorescent calcium indicator dyes have been developed for measurement of free intracellular calcium in eukaryotic cells. Here we report the use of one such dye, fura-2, for the study of intracellular calcium levels in the prokaryote Escherichia coli. Cells of E. coli were loaded with the membrane-permeable acetoxymethyl ester of fura-2, which was cleaved intracellularly to give the free pentaacid. The concentration of free [Ca2+]i in unstarved cells was maintained at 90 +/- 10 nM, irrespective of the Ca2+ concentration in the extracellular medium. Cells of a strain lacking the H+-translocating ATPase were depleted of endogenous energy reserves and loaded with calcium. In this strain oxidative phosphorylation is uncoupled, so ATP is not produced by respiration. In starved cells [Ca2+]i varied from 0.2 to 0.7 microM when the loading Ca2+ concentration varied from 10 microM to 10 mM. Addition of glucose lowered the Ca2+ levels to 90 nM. Addition of respiratory substrates as energy donors produced cyanide-sensitive efflux. Total cell Ca2+ increased in parallel to the extracellular calcium, but the pool of free calcium did not equilibrate with the total cellular pool. These results demonstrate that 1) the pool of total Ca2+ in the bacterial cell is large and responds to extracellular calcium, 2) the free [Ca2+]i is independent of extracellular calcium, and 3) energy in the form of a proton motive force is required for maintenance of the free intracellular pool of calcium.

Characterization of (Ca2+ + Mg2+)-ATPase of sarcoplasmic reticulum by laser-excited europium luminescence.

The molecular environment of Ca2+ translocating sites of skeletal muscle sarcoplasmic reticulum (SR) (Ca2+ + Mg2+)-ATPase has been studied by pulsed-laser excited luminescence of Eu3+ used as a Ca2+ analogue. Interaction of Eu3+ with SR was characterized by investigating its effect on partial reactions of the Ca2+ transport cycle. In native SR vesicles, Eu3+ was found to inhibit Ca2+ binding, phosphoenzyme formation, ATP hydrolysis activity and Ca2+ uptake in parallel fashion. The non-specific binding of Eu3+ to acidic phospholipids associated with the enzyme was prevented by purifying (Ca2+ + Mg2+)-ATPase and exchanging the endogenous lipids with a neutral phospholipid, dioleoylglycerophosphocholine. The results demonstrate that the observed inhibition of Ca2+ transport by Eu3+ is due to its binding to Ca2+ translocating sites. The 7F0----5D0 transition of Eu3+ bound to these sites was monitored. The non-Lorentzian nature of the excitation profile and a double-exponential fluorescence decay revealed the heterogeneity of the two sites. Measurement of fluorescence decay rates in H2O/D2O mixture buffers further distinguished the sites. The number of water molecules in the first co-ordination sphere of Eu3+ bound at transport sites were found to be 4 and 1.5. Addition of ATP reduced these numbers to zero and 0.6. These data show that the calcium ions in translocating sites are well enclosed by protein ligands and are further occluded down to zero or one water molecule of solvation during the transport process.

Estimation of inter-binding-site distances in sarcoplasmic reticulum (Ca2+ + Mg2+)-ATPase using Eu(III) luminescence energy transfer.

We have used several trivalent lanthanides as probes for the high-affinity Ca(II)-binding site of the Ca(II) + Mg(II)-ATPase of skeletal muscle sarcoplasmic reticulum. The luminescent probes Eu(III) and Tb(III) were excited directly with pulsed laser light and the energy transfer efficiencies to several lanthanide acceptors were measured, under conditions in which most donor-acceptor pair occupied high-affinity Ca(II) sites. We obtain an inter-ionic site distance of about 0.8-0.9 nm. Energy transfer measurements were also done with Eu(III) in at least one Ca(II) site and bidentate Cr-ATP complex at the ATP hydrolytic site. Quenching of Eu(III) luminescence by Cr-ATP was total under these conditions. We calculate an upper limit of 1.0 nm for the distance from the Ca(II) site(s) to the complexed Cr(III) ion at the hydrolytic site.

Synthesis and characterization of a peptide segment of (Ca2+ + Mg2+)-ATPase. A candidate for calcium transport site.

The second tryptic digestion (TD2) of the (Ca2+ + Mg2+)-ATPase results in the decrease of Ca2+ transport due to uncoupling and the alteration of one of the two high affinity sites to a low affinity site. The eight amino acids adjacent to the tryptic digestion site form a torus with two carboxylic side chains of one aspartic and one glutamic acid for the fast twitch skeletal ATPase and two aspartic acids for the slow twitch/cardiac ATPase toward the inside. The eight amino acid peptides were synthesized for both forms of the ATPase and their binding characteristics were studied with luminescent Eu3+ as a Ca2+ analogue. The data indicate that the peptide binds Eu3+ with 1.0 Eu3+/peptide and strips off two water molecules. The peptide region is a candidate for the Ca2+ transport site of the (Ca2+ + Mg2+)-ATPase.

Alteration in calcium-binding activity in synaptosomal membranes from rat brains in association with physical dependence upon ethanol.

The effects of ethanol treatment on calcium-binding activity in synaptosomal membrane fraction from rat brains were studied. The synaptosomal membrane fraction from the hippocampus, the cortex and the cerebellum from control, single dose (6 g/kg), dependent intoxicated (prodromal) and dependent withdrawing (ethanol withdrawal syndrome) rats were used. Two different methods were used for determining the calcium activity in these membrane preparations: the calcium chelator fluorescence probe, chlortetracycline (CTC), was used to measure Ca2+ binding sites in the membranes, and radioactive calcium (45Ca) was used to measure the calcium binding to the synaptosomal membranes. Both methods provided similar conclusions; the calcium activity was higher during the dependent intoxicated phase of the ethanol withdrawal period. The synaptosomal membranes from the hippocampus showed more drastic changes in calcium-binding activity than the cortex and the cerebellum. These results suggest that ethanol dependence is associated with changes in calcium-binding activity in certain areas of the rat brain.

Temperature dependent conformational changes in calmodulin.

Calcium bound calmodulin undergoes a reversible conformational change in the temperature range (22-28 degrees C). The transition temperature depends upon the concentration of calcium bound to calmodulin. The transition occurs at higher temperature (28 degrees C) in fully Ca2+ bound calmodulin as compared to those with lower Ca2+ concentrations (22 degrees C). The sequence of filling the four Ca2+ binding sites is different for the two temperature dependent conformers.