Sandflies of the south part of Ouagadougou City, Burkina Faso.

Since 1996, the number of cases of cutaneous leishmaniasis has increased dramatically in Ouagadougou. Leishmania major, zymodeme MON74 was the only strain isolated in this focus. An epidemiological study of the phlebotomine sandflies fauna has been undertaken. Collections of sandflies have been carried out in six areas of the town during one year with two intensive collections at the end of the dry (May-June) and wet seasons (September-October). The only species of genus Phlebotomus captured was P. duboscqi. This represented 11.2% from the 4,676 collected sandflies. P. duboscqi is a well known vector of L. major, nevertheless, none of the collected sandflies were infected with L. major. 16 species of Sergentomyia were present in the south area of Ouagadougou and S. schwetzi was the most abundant sandfly.

[Mycological examination of a non-uniseriate Fumigati section's Aspergillus]. / Isolement d'un Aspergillus section Fumigati d'apparence non-unisériée.

Aspergillus species are filamentous fungi of Ascomycete class. They are opportunistic pathogens frequently responsible for many severe infections. Among these species Aspergillus fumigatus is the most important human pathogen because of his thermophilic feature. Concerning microscopic morphology Aspergillus fumigatus is characterized by an asexual multiplication with a conidiophore formed by a conical-shaped vesicle. The phialides are directly attached to this clavated vesicle (uniseriate) and produce conidia chains. We report the case of mycological examination identifying a non uniseriate Aspergillus strain which belongs to Fumigati section.

Fatal-stroke syndrome revealing fungal cerebral vasculitis due to Arthrographis kalrae in an immunocompetent patient.

We report an uncommon clinical presentation of a unique case of fatal invasive fungal cerebral vasculitis due to Arthrographis kalrae in a nonimmunocompromised host. The identity of the fungus was determined by morphological characteristics and by analysis of internal transcribed spacer 1 sequences and was confirmed by postmortem examination of the brain tissues. Establishing rapidly the link between the clinical syndromes and the fungal infection of the central nervous system is essential to improve the outcome. As our case has shown, it is more challenging to make a diagnosis of fungal infection when there are no risk factors of immunodeficiency and when the clinical presentation seems uncommon.

[Phlebotomine sandflies (Diptera: Psychodidae) of French Guiana. I--Description of the male of Lutzomyia (Trichopygomyia) depaquiti n. sp]. / Les phlébotomes (Diptera: Psychodidae) de la Guyane française. I--Description du mâle de Lutzomyia (Trichopygomyia) depaquiti n. sp.

Description of a new tropical species of French Guiana: Lutzomyia (Trichopygomyia) depaquiti. The principal character is the aedeagus morphology that presents lateral expansions like a marine anchor. An other particularity is centered on the complex paramere with a lateral lobe. Holotype is deposited at the National Museum of Natural History of Paris.

Microsatellite marker analysis as a typing system for Candida glabrata.

Candida glabrata is one of the most important causes of nosocomial fungal infection. We investigated, using a multiplex PCR, three polymorphic microsatellite markers, RPM2, MTI, and ERG3, in order to obtain a rapid genotyping method for C. glabrata. One set of primers was designed for each locus, and one primer of each set was dye labeled to read PCR signals using an automatic sequencer. Eight reference strains including other Candida species and 138 independent C. glabrata clinical isolates were tested. The clinical isolates were collected from different anatomical sites of adult patients either hospitalized in different wards of two different hospitals or not hospitalized. Since C. glabrata is haploid, one single PCR product for each PCR set was obtained and assigned to an allele. The numbers of different alleles were 5, 7, and 15 for the RPM2, MTI, and ERG3 loci, respectively. The number of allelic associations was 21, leading to a discriminatory power of 0.84. The markers were stable after 25 subcultures, and the amplifications were specific for C. glabrata. A factorial correspondence analysis did not indicate any correlation between the 21 multilocus genotypes and the clinical data (source, sex, ward, anatomical sites). Microsatellite marker analysis is a rapid and reliable technique to investigate clinical issues concerning C. glabrata. However, its discriminatory power should be improved by testing other polymorphic microsatellite loci.

Phlebotomine sand flies from Ouagadougou, Burkina Faso: first record of Phlebotomus (Larroussius) longicuspis south of the Sahara.

During a brief entomological survey carried out in Ouagadougou, Burkina Faso, in March 2002, 178 phlebotomine sand flies (Diptera, Psychodidae) were caught using CDC miniature light traps. They were identified as Phlebotomus duboscqi Neveu-Lemaire, P. longicuspis Nitzulescu, Sergentomyia adleri (Theodor), S. affinis vorax (Parrot), S. antennata (Newstead), S. bedfordi (Newstead), S. christophersi (Sinton), S. fallax (Parrot), S. magna (Sinton) and S. schwetzi (Adler, Theodor & Parrot). This is the first record south of the Sahara of P. longicuspis sensu lato. This proven vector of Leishmania infantum Nicolle is a species closely related to P. perniciosus. It was identified by morphology and by the sequencing of a fragment of mitochondrial cytochrome b. There was 100% sequence homology with typical Tunisian specimens, and all characters identified the specimen as P. longicuspis sensu stricto. Epidemiological and biogeographical consequences are discussed.

Fatal Microascus trigonosporus (anamorph Scopulariopsis) pneumonia in a bone marrow transplant recipient.

Over the past decade, an increasing number of opportunistic mycelial fungal infections have been reported in immunocompromised patients. Presented here is the first reported case of Microascus trigonosporus pneumonia, which occurred in a 24-year-old-man with a history of allogenic bone marrow transplantation with graft-versus-host disease. Despite the administration of effective antifungal treatment, the patient died after uncontrollable respiratory failure and multiorgan failure developed. This report confirms the results of previous studies that suggested a very poor outcome for bone marrow transplant recipients with non-Aspergillus mould infections.

Action of adrenalin on the circulation of the murine Plasmodium developing stages, in different blood compartments.

Adrenalin was used to investigate in vivo the circulation of the different stages of rodent Plasmodium present in the blood. A single dose of adrenalin injected to mice infected with P. yoelii resulted immediately in i) a diminution of the parasitaemia of approximately 50% in the peripheral large vessels (estimated in tail blood films), as well as in the capillaries (estimated in smears of blood collected from a fed Anopheles), and ii) an increased parasitaemia in blood collected by cardiac puncture from the right heart. The numbers of young stages of P. yoelii in the peripheral blood were initially somewhat reduced but, unexpectedly, midterm trophozoites were preferentially expelled from the peripheral blood into major organs like the heart. With P. vinckei, parasitaemia decreased only when midterm trophozoites predominated, and with P. chabaudi no effect was observed at any time. We propose that midterm trophozoites, by their increased surface area, as compared to rings, and their flexibility which contrasts with the rigid schizonts, are particularly susceptible to haemodynamic perturbations.

Variation in murid Plasmodium desequestration and its modulation by stress and pentoxifylline.

Changes in the parasitaemia and the characteristics of parasitic infection for three species of rodent Plasmodium (P. chabaudi chabaudi, P. vinckei petteri and P. yoelii yoelii) were investigated under conditions of stress and after treatment with pentoxifylline (POF), a drug that increases red blood cell deformability and causes peripheral vasodilatation. The results indicated that under stress, late parasite stages became less abundant in the tail blood of mice. These changes might be the consequence of parasite sequestration. Attempts to assess sequestration intensity were made by measuring the release rate (RR) of late stages for 10,000 red blood cells. The RR is given by the product of the parasitaemia (P) by the percentage of old trophozoites (OT) and schizonts (S) in the peripheral blood: RR = P(%OT + %S) . With all three species, RR decreased considerably within 5 min following the manipulation of the mice. Injections of POF had the opposite effect. POF had a protective effect against infection by P.v. petteri, causing a delay of 48 h in the development of infection and a higher survival rate in treated mice.

Targeting of 3'-azido 3'-deoxythymidine (AZT)-loaded poly(isohexylcyanoacrylate) nanospheres to the gastrointestinal mucosa and associated lymphoid tissues.

PURPOSE: The aim of the studv was to evaluate the capacity of poly(isohexylcyanoacrylate) nanospheres to concentrate 3'-azido 3'-deoxythymidine (AZT) in the intestinal epithelium and associated immunocompetent cells, which are known to be one of the major reservoirs of the human immunodeficiency virus (HIV). METHODS: The tissue concentration of 3H-radiolabeled AZT in the gastrointestinal (GI) tract was obtained 30 and 9() minutes after intragastric administration to rats at a dose of 0.25 mg AZT/100 g of body weight. The distribution along the intestine was determined. AZT concentrations in the lymph were obtained by lymphatic duct cannulation. RESULTS: Unlike the solution. nanoparticles did concentrate AZT very cfficiently in the intestinal mucosa, as well as in the Peyer's patches, and could simultaneously control the release of free AZT. Concentration in Peyer's patches was 4 times higher for nanoparticles, compared with the control solution. The tissue concentration was 30-45 microM, which was much higher than the reported IC50 of AZT (0.06-1.36 microM) and was regularly distributed along the gastrointestinal tract. CONCLUSIONS: Nanoparticles have been shown to be efficient in concentrating AZT in the intestinal epithelium and gut-associated lymphoid tissues, supporting the view that these particles may represent a promising carrier to treat specifically the GI reservoir of HIV.

[Phlebotomines of the Isle of Cyprus. III. Species inventory ]. / Les phlébotomes de l'Ile de Chypre. III--Inventaire faunistique.

Three surveys on canine leishmaniasis were carried out in Cyprus (1993, 1998 and 1999) emphasise the presence of eleven species of phlebotomine sandflies: Phlebotomus (Phlebotomus) papatasi, P. (Paraphlebotomus) alexandri, P. (Pa.) jacusieli (first mention in Cyprus), P. (Pa.) sergenti, P. (Larroussius) galilaeus, P. (L.) tobbi, P. (Transphlebotomus) economidesi, P. (T.) mascittii, Sergentomyia (Sergentomyia) azizi (its specific statute is validated by the authors), S. (S.) fallax et S. (S.) minuta. P. (Adlerius) kyreniae was not caught during the surveys. The authors propose hypothesis of settlement of the island by phlebotomine sandflies according this species inventory. A first migration period took probably place during the Miocene time and a second one during the Pleistocene time.

[Phlebotomine sandflies (Diptera-Psychodidae) of the isle of Cyprus. II--Isolation and typing of Leishmania (Leishmania infantum Nicolle, 1908 (zymodeme MON 1) from Phlebotomus (Larroussius) tobbi Adler and Theodor, 1930]. / Les phlébotomes (Diptera-Psychodidae) de l'île de Chypre. II--Présence de Leishmania (Leishmania) infantum Nicolle, 1908 (zymodeme MON1) chez Phlebotomus (Larroussius) tobbi Adler et Theodor, 1930.

During two surveys conducted in Cyprus (August 1998 and September 1999), 2,910 phlebotomine sandflies females were caught by CDC miniature light traps then dissected under binocular and examined on microscope. Eleven species were identified: Phlebotomus papatasi, P. sergenti, P. jacusieli, P. alexandri, P. tobbi, P. galilaeus, P. mascittii, P. economidesi, Sergentomyia fallax, S. minuta et S. azizi. The Larroussius species (P. galilaeus and P. tobbi) are the most abundant (more than 60% of our captures). Promastigotes were isolated from one specimen identified as P. tobbi. A Leishmania stock was successfully cultured and identified by isoenzyme characterisation as belonging to L. infantum zymodeme MON 1. The same zymodeme was isolated and identified from four dogs too. Because of the absence of usual vectors of L. infantum in the eastern part of the Mediterranean basin (P. neglectus and P. syriacus), and according to its distribution in Cyprus, P. tobbi constitute certainly a good local vector. It seems to be not very anthropophilic, that could explain the very few human cases.

Semisynthesis and cytotoxicity of amino acetogenins and derivatives.

Semisynthetic derivatives were prepared from two natural annonaceous acetogenins, rolliniastatin-1 and squamocin, and their cytotoxicity was evaluated. Amino derivatives show decreased bioactivity. Isorolliniastatin-1 was found to be much less toxic than rolliniastatin-1 after intraperitoneal administration to mice, although the in vitro cytotoxicity of both compounds was comparable.

Synthesis and antimalarial activities of fluoroalkyl derivatives of dihydroartemisinin.

Fluoroalkyl ethers (4) of dihydroartemisinin (2) have been prepared by reaction of fluoroalkyl alcohols with dihydroartemisinin by different methods (BF3,Et2O or TMSCl catalysis or Mitsunobu reaction). Ethers 4a-d derived from primary fluoroalkyl alcohols were obtained in moderate to good yields by these methods. Ethers 4e-j have been prepared from fluoroalkyl secondary and tertiary alcohols and phenol using the Mitsunobu reaction. Although in vitro antimalarial activities of ethers toward Plasmodium falciparum W-2 asiatic strain are moderate, in vivo activities against Plasmodium berghei (NT 173) are excellent.

Determination of 2-n-propylquinoline in mouse plasma and liver by high-performance liquid chromatography.

A high-performance liquid chromatographic method was developed for the specific determination of 2-n-propylquinoline, a new anti-leishmaniasis drug, in plasma and liver homogenates of mice. 2-n-Propylquinoline was extracted with methyl-tert.-butyl ether with quinoline as internal standard. Separation was carried out using a Nucleosil C18 column. The mobile phase consisted of methanol-0.005 M ammonium acetate buffer (60:40) at pH 5.5 and 8 for plasma and liver homogenates, respectively. Detection was monitored at 233 nm. The method was validated and shown to be accurate and precise for plasma and liver homogenates. Extraction yield was 96% in plasma and 81% in liver homogenates. This method was used to determine the pharmacokinetic profile of 2-n-propylquinoline following oral administration to mice.

Enhanced gametocyte formation by Plasmodium chabaudi in immature erythrocytes: pattern of production, sequestration, and infectivity to mosquitoes.

The objective of this study was to investigate the chronobiology and infectivity of the gametocytes of Plasmodium chabaudi chabaudi. In order to increase the production of gametocytes, mice were treated with phenylhydrazine to induce a hyper-reticulocytosis. The authors observed an important stimulation of gametocytogenesis. Gametocytes were seen as soon as the second day postinoculation and were produced periodically at each schizogony, every 24 hr. The gametocytic developmental cycle lasted 60 hr and consisted of 4 successive stages: stage 0 at 36 hr, from merozoite invasion, stage I at 42 hr, stage II at 48 hr, and stage III at 54 hr. An important fraction of stage II was sequestered in small peripheral capillaries. The numbers of oocysts in the mosquitoes fed on phenylhydrazine-treated mice were larger than in controls. When mosquitoes were fed at different times of the day, circadian differences in the oocyst counts were not statistically significant. However, stage II was considered to be probably the most infective stage because, like the infective gametocyte stage of other species of murine malaria, it is sequestered in the peripheral capillaries. In contrast with Plasmodium vinckei, there is no peak of infectivity at the time of sequestration of the infective stage; this is probably due to the inhibitory effect of the schizogony occurring at this time.

The gametocytes of Plasmodium vinckei petteri, their morphological stages, periodicity and infectivity.

Gametocyte production by P. vinckei petteri was cyclic, occurring at each schizogony every 24 h. They matured in 27 h from merozoite to type 0 microgametocyte, in 3 h from type 0 to type I, 6 h from type I to type II and 3 h from type II to type III. Transmission experiments showed that the time of maximum infectivity was midday when mice were inoculated at midnight, and midnight when mice were inoculated at midday. In all instances, maximum infectivity coincided with a peak in intensity by type II microgametocytes, a relationship confirmed by multiple correspondence analysis. The proportion of type II microgametocytes was higher in the mosquitoes blood meal than in smears of tail blood of mice, suggesting a sequestration phenomenon with this stage.

The effect of some 2-substituted quinolines isolated from Galipea longiflora on Plasmodium vinckei petteri infected mice.

We have evaluated the in vivo antiplasmodial activity of six 2-substituted quinolines and a total alkaloidal extract of Galipea longiflora. BALB/c mice infected with Plasmodium vinckei petteri were treated orally at single dose of 50 mg/kg with quinolines or extract. Contrary to the previous results obtained with the Leishmania murine infection, 2-n-pentylquinoline showed activity against P. vinckei petteri. This result seems to confirm the antimalarial efficacy of infused stem bark of G. longiflora.

[Phlebotomines of Nicaragua. II. Description of Lutzomyia legerae n. sp. (Diptera:Psychodidae)]. / Phlébotomes du Nicaragua. II. Description de Lutzomyia legerae n. sp. (Diptera:Psychodidae).

Both sexes of Lutzomyia legerae n. sp., a new species of phlebotomine sand fly belonging to the subgenus Lutzomyia, cruciata complex, are described from specimens collected in peridomestic environment, by light-trap, in central Nicaragua. Females of the new species are very close to L. cruciata. Therefore, in the leishmaniasis foci where the two species occur sympatrically implication of each species as a vector has to be carefully investigated.

Synthesis of an antileishmanial alkaloid isolated from Galipea longiflora and of related compounds.

Synthesis of an antileishmanial alkaloid and related compounds by using various epoxide-forming reactions is described.