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1.
J Environ Manage ; 365: 121550, 2024 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-38908154

RESUMO

In light of the escalating global climate risks threatening human survival, there is a global consensus on the necessity for collaborative reduction of pollutant and carbon emissions (CRPC). Within this context, digital inclusive finance (DIF) is recognized for its unique inclusiveness and digital characteristics as a critical factor in promoting environmentally friendly and sustainable development. DIF provides advantageous channels for environmental governance, thereby making the achievement of CRPC objectives feasible. However, the impact of DIF on CRPC has not been fully explored. This study employs a spatial econometric model to investigate the impact of DIF on CRPC in 278 prefecture-level cities in China from 2011 to 2020. The findings indicate that DIF has a positive impact on CRPC, with significant spatial spillover effects. The analysis highlights the pivotal mediating roles played by technology effect and electrified effect of the energy mix, while environmental regulation effect plays a moderating role. Notably, disparities in the impact of DIF on CRPC are evident, particularly in non-resource-based cities, cities with low carbon intensity, and eastern regions where spatial spillover effects are more pronounced. These experiences enrich the relevant thesis in terms of DIF on CRPC, providing a theoretical basis for formulating CRPC schemes.

2.
J Physiol ; 602(6): 1175-1197, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38431908

RESUMO

Non-invasive transcranial direct-current stimulation (tDCS) is a safe ischaemic stroke therapy. Cathodal bilateral tDCS (BtDCS) is a modified tDCS approach established by us recently. Because selenium (Se) plays a crucial role in cerebral ischaemic injury, we investigated whether cathodal BtDCS conferred neuroprotection via regulating Se-dependent signalling in rat cerebral ischaemia-reperfusion (I/R) injury. We first showed that the levels of Se and its transport protein selenoprotein P (SEPP1) were reduced in the rat cortical penumbra following I/R, whereas cathodal BtDCS prevented the reduction of Se and SEPP1. Interestingly, direct-current stimulation (DCS) increased SEPP1 level in cultured astrocytes subjected to oxygen-glucose deprivation reoxygenation (OGD/R) but had no effect on SEPP1 level in OGD/R-insulted neurons, indicating that DCS may increase Se in ischaemic neurons by enhancing the synthesis and secretion of SEPP1 in astrocytes. We then revealed that DCS reduced the number of injured mitochondria in OGD/R-insulted neurons cocultured with astrocytes. DCS and BtDCS prevented the reduction of the mitochondrial quality-control signalling, vesicle-associated membrane protein 2 (VAMP2) and syntaxin-4 (STX4), in OGD/R-insulted neurons cocultured with astrocytes and the ischaemic brain respectively. Under the same experimental conditions, downregulation of SEPP1 blocked DCS- and BtDCS-induced upregulation of VAMP2 and STX4. Finally, we demonstrated that cathodal BtDCS increased Se to reduce infract volume following I/R. Together, the present study uncovered a molecular mechanism by which cathodal BtDCS confers neuroprotection through increasing SEPP1 in astrocytes and subsequent upregulation of SEPP1/VAMP2/STX4 signalling in ischaemic neurons after rat cerebral I/R injury. KEY POINTS: Cathodal bilateral transcranial direct-current stimulation (BtDCS) prevents the reduction of selenium (Se) and selenoprotein P in the ischaemic penumbra. Se plays a crucial role in cerebral ischaemia injury. Direct-current stimulation reduces mitochondria injury and blocks the reduction of vesicle-associated membrane protein 2 (VAMP2) and syntaxin-4 (STX4) in oxygen-glucose deprivation reoxygenation-insulted neurons following coculturing with astrocytes. Cathodal BtDCS regulates Se/VAMP2/STX4 signalling to confer neuroprotection after ischaemia.


Assuntos
Isquemia Encefálica , Traumatismo por Reperfusão , Selênio , Acidente Vascular Cerebral , Estimulação Transcraniana por Corrente Contínua , Ratos , Animais , Isquemia Encefálica/terapia , Isquemia Encefálica/metabolismo , Neuroproteção/fisiologia , Proteína 2 Associada à Membrana da Vesícula , Selenoproteína P , Oxigênio/metabolismo , Traumatismo por Reperfusão/prevenção & controle , Traumatismo por Reperfusão/metabolismo , Glucose/metabolismo , Proteínas Qa-SNARE
3.
iScience ; 27(1): 108688, 2024 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-38188517

RESUMO

RNA splicing is a post-transcriptional event that regulates many physiological and pathological events. However, whether RNA splicing regulates cerebral I/R-induced brain injury remains largely unknown. In this study, we found that the chromatin target of Prmts (CHTOP) was highly expressed in neurons, and anti-inflammatory cytokine interleukin-10 (IL-10) upregulates its expression after ischemia. In addition, overexpression or knockdown of CHTOP alleviated or exacerbated neuronal death in both experimental stroke mice and cultured neurons. Mechanistically, RNA alternative splicing is altered early after oxygen and glucose deprivation/reoxygenation (OGD/R). CHTOP interacted with nuclear speckle-related proteins to regulate alternative mRNA splicing of neuronal survival-related genes after OGD/R. In addition, I/R injury-induced cytokines IL-10 regulate CHTOP-mediated RNA splicing to alleviate ischemic brain injury. Taken together, this study reveals the alteration of RNA splicing after OGD/R and identifies the IL-10-CHTOP-RNA splicing axis as a modulator of brain injury, which may be promising therapeutic targets for ischemic stroke.

4.
Mol Neurobiol ; 61(4): 2033-2048, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-37843800

RESUMO

Surfeit locus protein 4 (SURF4) functions as a cargo receptor that is capable of transporting newly formed proteins from the lumen of the endoplasmic reticulum into vesicles and Golgi bodies. However, the role of SURF4 in the central nervous system remains unclear. The aim of this study is to investigate the role of SURF4 and its underlying mechanisms in cerebral ischemia/reperfusion (I/R) injury in rats, and whether it can be used effectively for novel therapeutic intervention. We also examined whether transcranial direct-current stimulation (tDCS) can exert a neuroprotective effect via SURF4-dependent signalling. Following cerebral I/R injury in rats, a significant increase was observed in the expression of SURF4. In both I/R injury and oxygen-glucose deprivation (OGD) insult, suppressing the expression of SURF4 demonstrated a neuroprotective effect, while overexpression of SURF4 resulted in increased neuronal death. We further showed that the levels of nerve growth factor precursor (proNGF), p75 neurotrophin receptor (p75NTR), sortilin, and PTEN were increased following cerebral I/R injury, and that SURF4 acted through the PTEN/proNGF signal pathway to regulate neuronal viability. We demonstrated that tDCS treatment reduced SURF4 expression and decreased the infarct volume after cerebral I/R injury. Together, this study indicates that SURF4 plays a critical role in ischemic neuronal injury and may serve as a molecular target for the development of therapeutic strategies in acute ischemic stroke.


Assuntos
Isquemia Encefálica , AVC Isquêmico , Fármacos Neuroprotetores , Traumatismo por Reperfusão , Estimulação Transcraniana por Corrente Contínua , Ratos , Animais , Fármacos Neuroprotetores/farmacologia , Oxigênio/metabolismo , Traumatismo por Reperfusão/metabolismo , Isquemia Encefálica/metabolismo , Apoptose , Infarto da Artéria Cerebral Média/metabolismo
5.
Brain Res ; 1825: 148724, 2024 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-38110073

RESUMO

Phosphoglycerate kinase 1 (PGK1) is extensively located in the cytosol and mitochondria. The role of PGK1 in ischemic neuronal injury remains elusive. In the in vitro model of oxygen-glucose deprivation/reoxygenation (OGD/R), we showed that PGK1 expression was increased in cortical neurons. Knockdown of PGK1 led to a reduction of OGD/R-induced neuronal death. The expression of cytosolic PGK1 was reduced, but the levels of mitochondrial PGK1 were increased in OGD/R-insulted neurons. Inhibiting the activity of mitochondrial PGK1 alleviated the neuronal injury after OGD/R insult. We further showed that the protein levels of TBC domain family member 15 (TBC1D15) were decreased in OGD/R-insulted neurons. Knockdown of TBC1D15 led to increased levels of mitochondrial PGK1 after OGD/R insult in cortical neurons. Moreover, increased reactive oxygen species (ROS) resulted in a reduction of TBC1D15 in OGD/R-insulted neurons. These results suggest that the upregulation of mitochondrial PGK1 by ROS-TBC1D15 signaling pathway promotes neuronal death after OGD/R injury. Mitochondrial PGK1 may act as a regulator of neuronal survival and interventions in the PGK1-dependent pathway may be a potential therapeutic strategy.


Assuntos
Oxigênio , Traumatismo por Reperfusão , Humanos , Oxigênio/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Regulação para Cima , Glucose/metabolismo , Mitocôndrias/metabolismo , Apoptose , Traumatismo por Reperfusão/metabolismo , Proteínas Ativadoras de GTPase/metabolismo , Fosfoglicerato Quinase/metabolismo
6.
Mol Neurobiol ; 60(11): 6715-6730, 2023 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-37477767

RESUMO

Humans exhibit a rich intestinal microbiome that contain high levels of bacteria capable of producing 3-oxo-lithocholic acid (3-oxoLCA) and other secondary bile acids (BAs). The molecular mechanism mediating the role of 3-oxoLCA in cerebral ischemia-reperfusion (I/R) injury remains unclear. We investigated the role of 3-oxoLCA in a rat cerebral I/R injury model. We found that the concentrations of 3-oxoLCA within the cerebrospinal fluid were increased following I/R. In the in vitro oxygen-glucose deprivation (OGD) model, the levels of intraneuronal 3-oxoLCA was elevated following OGD insult. We showed that the increase of membrane ASBT (apical sodium-dependent bile acid transporter) contributed to OGD-induced elevation of intraneuronal 3-oxoLCA. Increasing intraneuronal 3-oxoLCA promoted ischemia-induced neuronal death, whereas reducing 3-oxoLCA levels were neuroprotective. Our results revealed that PLOD2 (procollagen-lysine, 2-oxoglutarate 5-dioxygenases 2) functioned upstream of PTEN (the phosphatase and tensin homolog deleted on chromosome 10) and downstream of 3-oxoLCA to promote OGD-induced neuronal injury. We further demonstrated that direct-current stimulation (DCS) decreased the levels of intraneuronal 3-oxoLCA and membrane ASBT in OGD-insulted neurons, while bilateral transcranial DCS (tDCS) reduced brain infarct volume following I/R by inhibiting ASBT. Together, these data suggest that increased expression of ASBT promotes neuronal death via 3-oxoLCA-PLOD2-PTEN signaling pathway. Importantly, bilateral tDCS suppresses ischemia-induced increase of ASBT, thereby conferring neuroprotection after cerebral I/R injury.


Assuntos
Isquemia Encefálica , Traumatismo por Reperfusão , Estimulação Transcraniana por Corrente Contínua , Humanos , Ratos , Animais , Neuroproteção , Transdução de Sinais , Isquemia Encefálica/metabolismo , Oxigênio/metabolismo , Infarto Cerebral , Glucose/metabolismo , Traumatismo por Reperfusão/metabolismo , Apoptose , Pró-Colágeno-Lisina 2-Oxoglutarato 5-Dioxigenase/metabolismo , PTEN Fosfo-Hidrolase/metabolismo
7.
Eur J Neurosci ; 58(5): 3330-3346, 2023 09.
Artigo em Inglês | MEDLINE | ID: mdl-37452630

RESUMO

Isoleucine is a branched chain amino acid. The role of isoleucine in cerebral ischemia-reperfusion injury remains unclear. Here, we show that the concentration of isoleucine is decreased in cerebrospinal fluid in a rat model of cerebral ischemia-reperfusion injury, the rat middle cerebral artery occlusion (MCAO). To our surprise, the level of intraneuronal isoleucine is increased in an in vitro model of cerebral ischemia injury, the oxygen-glucose deprivation (OGD). We found that the increased activity of LAT1, an L-type amino acid transporter 1, leads to the elevation of intraneuronal isoleucine after OGD insult. Reducing the level of intraneuronal isoleucine promotes cell survival after cerebral ischemia-reperfusion injury, but supplementing isoleucine aggravates the neuronal damage. To understand how isoleucine promotes ischemia-induced neuronal death, we reveal that isoleucine acts upstream to reduce the expression of CBFB (core binding factor ß, a transcript factor involved in cell development and growth) and that the phosphatase PTEN acts downstream of CBFB to mediate isoleucine-induced neuronal damage after OGD insult. Interestingly, we demonstrate that direct-current stimulation reduces the level of intraneuronal isoleucine in cortical cultures subjected to OGD and that transcranial direct-current stimulation (tDCS) decreases the cerebral infarct volume of MCAO rat through reducing LAT1-depencent increase of intraneuronal isoleucine. Together, these results lead us to conclude that LAT1 over activation-dependent isoleucine-CBFB-PTEN signal transduction pathway may mediate ischemic neuronal injury and that tDCS exerts its neuroprotective effect by suppressing LAT1 over activation-dependent signalling after cerebral ischemia-reperfusion injury.


Assuntos
Isquemia Encefálica , Fármacos Neuroprotetores , Traumatismo por Reperfusão , Estimulação Transcraniana por Corrente Contínua , Ratos , Animais , Isoleucina/farmacologia , Neuroproteção , Isquemia Encefálica/metabolismo , Transdução de Sinais , Infarto da Artéria Cerebral Média/metabolismo , Traumatismo por Reperfusão/metabolismo , Fármacos Neuroprotetores/farmacologia , Oxigênio
8.
Mol Neurobiol ; 59(12): 7423-7438, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36190692

RESUMO

Propionic acid (PPA) is a critical metabolite involved in microbial fermentation, which functions to reduce fat production, inhibit inflammation, and reduce serum cholesterol levels. The role of PPA in the context of cerebral ischemia-reperfusion (I/R) injury has yet to be clarified. Increasing evidence indicate that transcranial direct-current stimulation (tDCS) is a safe approach that confers neuroprotection in cerebral ischemia injury. Here, we show that the levels of PPA were reduced in the ischemic brain following a rat cerebral I/R injury and in the cultured rat cortical neurons after oxygen-glucose deprivation (OGD), an in vitro model of ischemic injury. We found that the decreased levels of transporter protein monocarboxylate transporter-1 (MCT1) were responsible for the OGD-induced reduction of PPA. Supplementing PPA reduced ischemia-induced neuronal death after I/R. Moreover, our results revealed that the neuroprotective effect of PPA is mediated through downregulation of phosphatase PTEN and subsequent upregulation of Lon protease 1 (LONP1). We demonstrated that direct-current stimulation (DCS) increased MCT1 expression and PPA level in OGD-insulted neurons, while tDCS decreased the brain infarct volume in the MCAO rats via increasing the levels of MCT1 expression and PPA. This study supports a potential application of tDCS in ischemic stroke.


Assuntos
Isquemia Encefálica , Fármacos Neuroprotetores , Protease La , Traumatismo por Reperfusão , Estimulação Transcraniana por Corrente Contínua , Animais , Ratos , Isquemia Encefálica/metabolismo , Infarto Cerebral , Glucose/metabolismo , Neuroproteção , Fármacos Neuroprotetores/farmacologia , Oxigênio/metabolismo , Protease La/metabolismo , PTEN Fosfo-Hidrolase/metabolismo , Traumatismo por Reperfusão/metabolismo
9.
Front Pharmacol ; 13: 950376, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36046828

RESUMO

Background: We and others have previously demonstrated that glycine is neuroprotective in cerebral ischemia-reperfusion injury. But glycine has low permeability to the blood-brain barrier (BBB). To deliver glycine into the ischemic brain to confer neuroprotection, we designed a novel glycine-containing and BBB-permeable tripeptide, the H-glycine-cysteine-phenylalanine-OH (GCF). Methods: For the synthesis of GCF, phenylalanine was included to increase the BBB permeability of the tripeptide. Cysteine was conjugated with glycine to enable the release of glycine from GCF. With the use of immunofluorescence labeling and HPLC assays, we measured the distribution and level of GCF. We used TTC labeling, LDH release, and MTT assays to evaluate the neuroprotective effect of GCF. Results: Following intravenous injection in a rat model of cerebral ischemia-reperfusion injury, GCF was intensively distributed in the ischemic neurons. Intravenous injection of GCF, but not the non-cleavable acetyl-GCF, resulted in the elevation of glycine in the ischemic brain. GCF but not acetyl-GC conferred neuroprotection in ischemic stroke animals. Conclusion: GCF protects against cerebral ischemia-reperfusion injury in the rat. In contrast to peptide drugs that exert therapeutic effect by interfering with signaling interaction, GCF acts as a BBB shuttle and prodrug to deliver glycine to confer neuroprotection, representing a novel therapeutic strategy for acute ischemic stroke.

10.
J Stroke Cerebrovasc Dis ; 31(8): 106570, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35636226

RESUMO

Aquaporin 4 (AQP4), a water channel protein, has been well studied in arterial stroke-induced brain edema. However, the role of AQP4 in cerebral venous sinus thrombosis (CVST) has not been reported. Here, we showed that AQP4 expression was increased in the brain of a rat CVST model, whereas inhibition of AQP4 decreased cerebral edema. Subsequent experiments showed that Shp-1 (Src homology 2-containing phosphatase-1) expression and NF-κB phosphorylation were upregulated after CVST. We found that Shp-1 inhibition resulted in enhancement of NF-κB activation and increased AQP4 expression accompanied by aggravated brain edema. We further showed that NF-κB inhibition led to decreased AQP4 expression and subsequent attenuation of brain edema but had no significant effect on Shp-1 expression. These results provide the first evidence suggesting that downregulation of NF-κB by Shp-1 alleviates CVST-induced brain edema through suppression of AQP4.


Assuntos
Edema Encefálico , Proteína Tirosina Fosfatase não Receptora Tipo 6/metabolismo , Trombose dos Seios Intracranianos , Animais , Aquaporina 4/metabolismo , Edema Encefálico/tratamento farmacológico , Edema Encefálico/etiologia , Edema Encefálico/prevenção & controle , Regulação para Baixo , NF-kappa B/metabolismo , Ratos , Trombose dos Seios Intracranianos/tratamento farmacológico , Trombose dos Seios Intracranianos/etiologia
11.
ACS Appl Mater Interfaces ; 13(42): 50246-50257, 2021 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-34637262

RESUMO

Hypoxia, induced by inadequate oxygen supply, is a key indication of various major illnesses, which necessitates the need to develop new nanoprobes capable of sensing hypoxia environments for the targeted system monitoring and drug delivery. Herein, we report a hypoxia-responsive, periodic mesoporous organosilica (PMO) nanocarrier for repairing hypoxia damage. ß-cyclodextrin (ß-CD) capped azobenzene functionalization on the PMO surface could be effectively cleaved by azoreductase under a hypoxia environment. Moreover, the nanosystem is equipped with fluorescence resonance energy transfer (FRET) pair (tetrastyrene derivative (TPE) covalently attached to the PMO framework as the donor and Rhodamine B (RhB) in the mesopores as the receptor) for intracellular visualization and tracking of drug release in real-time. The design of intelligent nanocarriers capable of simultaneous reporting and treating of hypoxia conditions highlights a great potential in the biomedical domain.


Assuntos
Hipóxia/tratamento farmacológico , Compostos de Organossilício/farmacologia , Linhagem Celular Tumoral , Portadores de Fármacos/síntese química , Portadores de Fármacos/química , Portadores de Fármacos/farmacologia , Liberação Controlada de Fármacos , Humanos , Hipóxia/metabolismo , Teste de Materiais , Estrutura Molecular , Compostos de Organossilício/síntese química , Compostos de Organossilício/química , Tamanho da Partícula , Porosidade , Propriedades de Superfície , Fatores de Tempo
12.
Mol Neurobiol ; 58(10): 4906-4920, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34218417

RESUMO

SET domain protein 3 (SETD3) is an actin-specific methyltransferase, a rare post-translational modification with limited known biological functions. Till now, the function of SETD3 in cerebral ischemia-reperfusion (I/R)-induced injury remains unknown. Here, we show that the protein level of SETD3 is decreased in rat neurons after cerebral I/R injury. SETD3 promotes neuronal survival after both glucose and oxygen deprivation/reoxygenation (OGD/R) and cerebral I/R injury, and knockdown of SETD3 increases OGD/R-induced neuronal death. We further show that OGD/R-induced downregulation of SETD3 leads to the decrease of cellular ATP level, the reduction of mitochondrial electric potential and the increase of ROS production, thereby promoting mitochondrial dysfunction. We found that SETD3 reduction-induced mitochondrial dysfunction is mediated by the suppression of actin polymerization after OGD/R. Furthermore, we demonstrate that I/R-induced upregulation of PTEN leads to the downregulation of SETD3, and suppressing PTEN protects against ischemic neuronal death through downregulation of SETD3 and enhancement of actin polymerization. Together, this study provides the first evidence suggesting that I/R-induced downregulation of SETD3 mediates PTEN upregulation-induced ischemic neuronal death through downregulation of SETD3 and subsequent suppression of actin polymerization. Thus, upregulating SETD3 is a potential approach for the development of ischemic stroke therapy.


Assuntos
Actinas/metabolismo , Morte Celular/fisiologia , Histona Metiltransferases/metabolismo , Mitocôndrias/metabolismo , Neurônios/metabolismo , PTEN Fosfo-Hidrolase/metabolismo , Animais , Isquemia Encefálica/metabolismo , Morte Celular/efeitos dos fármacos , Células Cultivadas , Regulação para Baixo/efeitos dos fármacos , Regulação para Baixo/fisiologia , Feminino , Histona Metiltransferases/antagonistas & inibidores , Masculino , Mitocôndrias/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Polimerização/efeitos dos fármacos , RNA Interferente Pequeno/administração & dosagem , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/metabolismo , Regulação para Cima/efeitos dos fármacos , Regulação para Cima/fisiologia
13.
World Neurosurg ; 127: 165-175, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30954748

RESUMO

There are some controversies about the surgical treatment strategy of mirror aneurysms. Whether to choose 1-stage or 2-stage surgery, bilateral or unilateral craniotomy, or surgical or interventional treatment are the main points in dispute. In this review, the different surgery strategies faced by patients are discussed. Different surgical methods are adopted based on the patient's individual state and the location and size of the aneurysm. A new imaging method is introduced using 3D Slicer, which clearly recognizes the relationship among aneurysm, brain tissue, skull, and nerve. The 3D Slicer can help surgeons undertake adequate preoperative preparation. In addition, we also introduce some ruptured factors (e.g., age, gender, hypertension, morphologic, and hemodynamic) concerning mirror aneurysm. Systematic discussion of the controversies and methods in surgical treatment of mirror aneurysms may provide new perspectives in future research for the prevention and treatment of mirror aneurysms.


Assuntos
Aneurisma Roto/epidemiologia , Aneurisma Roto/cirurgia , Gerenciamento Clínico , Aneurisma Intracraniano/epidemiologia , Aneurisma Intracraniano/cirurgia , Aneurisma Roto/diagnóstico por imagem , Humanos , Imageamento Tridimensional/métodos , Aneurisma Intracraniano/diagnóstico por imagem , Estudos Retrospectivos , Fatores de Risco
14.
J Biomed Mater Res B Appl Biomater ; 107(6): 2040-2049, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-30556953

RESUMO

The bioinert characteristic of polycaprolactone (PCL) remains a major defect when using PCL-based materials as small-diameter vascular grafts for implantation in vivo. To improve the hydrophilicity, cytocompatibility, and biodegradation of PCL vascular grafts, we constructed hybrid vascular grafts from electrospun PCL/gelatin. The PCL/gelatin hybrid vascular graft was further functionalized with the addition of heparin, to improve hemocompatibility. This study investigated the performance of heparin-loaded PCL/gelatin hybrid vascular grafts in vivo. Our results showed that the combination of gelatin and PCL was an effective approach to overcome each individual component's shortcomings, in the context of vascular graft generation. Furthermore, by loading heparin onto the grafts, thrombosis, which could be otherwise induced by gelatin, was prevented. Overall, our electrospun heparin-loaded PCL/gelatin vascular grafts promoted endothelialization and regulated smooth muscle regeneration. The data presented here show PCL/gelatin and heparin-loaded vascular grafts as an effective biomaterial candidate for the generation of artificial small-diameter vascular grafts. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 2040-2049, 2019.


Assuntos
Prótese Vascular , Endotélio Vascular/fisiologia , Gelatina/química , Heparina , Teste de Materiais , Músculo Liso Vascular/fisiologia , Poliésteres/química , Regeneração , Animais , Heparina/química , Heparina/farmacologia , Masculino , Ratos , Ratos Sprague-Dawley
15.
Regen Biomater ; 5(2): 105-114, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29644092

RESUMO

Small-diameter (<6 mm) vascular grafts are increasingly needed in peripheral vascular surgery but have few successes because of acute thrombosis, incomplete endothelialization and intimal hyperplasia after implantation. This study used electrospun poly(ε-caprolactone) as the matrix material. Heparin and selenium-containing catalyst-organoselenium modified polyethyleneimine were introduced through layer-by-layer assembly in order to build a vascular graft with in situ nitric oxide (NO) generation. The aim of this study was to explore the application of the graft with improved histocompatibility and biological function for vascular implantation in rats. After implantation in rats, compared to poly(ε-caprolactone), the modified grafts could promote the adhesion and proliferation of endothelial cells, and inhibit the adhesion of smooth muscle cells. The modified grafts remarkably promoted endothelialization, inhibited intimal hyperplasia and increased the ratio of alternatively activated macrophages (M2) to classical activated macrophages (M1). This work constructed a vascular graft with heparinization and catalytic NO generation for improving the vascularization, and accelerating the tissue regeneration by regulating the inflammatory response. The present study indicates that it is a promising method for regulating response and tissue regeneration of small diameter vascular grafts by a novel approach of combining heparinization and catalytic NO generation.

16.
Mater Sci Eng C Mater Biol Appl ; 84: 1-11, 2018 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-29519417

RESUMO

As an effective clinic treatment for cardiovascular disease, vascular transplantation gains much acceptance recently. However, due to the acute thrombosis and intimal hyperplasia, long-term failure of synthetic grafts after implanted in small diameter blood vessel decelerates its commercial use. The continued acute inflammation and delayed endothelialization have been considered as fundamental reasons. To enhance the adhesion and organization of endothelial cells (ECs) and improve the vascular remodeling process, we have constructed a vascular graft based on electrospun polycaprolactone (PCL) matrix, on which organoselenium-immobilized polyethyleneimine (SePEI) for in situ nitric oxide (NO) generation and hyaluronic acid (HA) grafted with poly (ethylene glycol) (PEG) modified Tyr-Ile-Gly-Ser-Arg (YIGSR) for antifouling and EC adhesion were deposited through electrostatic layer-by-layer assembly. The in vitro results showed that SePEI deposited on the grafts could catalyze stable generation of NO. After in situ implantation in rats for 4 and 8weeks, the graft promoted the transformation of macrophages into an anti-inflammatory phenotype (M2), which helped endothelium remodeling. YIGSR on the outmost layer facilitated more rapid and organized EC adhesion compared to PCL and non-modified grafts. PEG polymer chain on the outmost layer mitigated nonspecific adsorption of undesirable blood components. In our study, we first demonstrated the regulation of macrophage polarization by an NO-generating vascular graft. The results indicated that the approach of anti-inflammatory macrophage polarization and enhanced endothelialization through NO generation and PEG-modified YIGSR in our study may provide a new perspective for the clinic application of cell-free small-diameter vascular grafts.


Assuntos
Óxido Nítrico/metabolismo , Oligopeptídeos/química , Polietilenoglicóis/química , Animais , Antígenos CD/metabolismo , Antígenos de Diferenciação Mielomonocítica/metabolismo , Adesão Celular , Polaridade Celular , Proliferação de Células , Endotélio Vascular/citologia , Endotélio Vascular/metabolismo , Endotélio Vascular/patologia , Macrófagos/citologia , Macrófagos/metabolismo , Masculino , Camundongos , Óxido Nítrico/química , Poliésteres/química , Células RAW 264.7 , Ratos , Ratos Sprague-Dawley , Eletricidade Estática , Enxerto Vascular
17.
J Mater Chem B ; 6(18): 2795-2802, 2018 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-32254232

RESUMO

Rapid in vivo cellularization of implanted grafts is crucial to tissue regeneration in tissue engineering. The compositions and structures of the extracellular matrix (ECM) are important in regulating cell attachment, proliferation and migration. ECM remodeling, especially degradation, is closely related to cell migration under physiological and pathological conditions. Matrix metalloproteinases-1 (MMP-1, Collagenase I) could degrade collagen I in the ECM. So we put forward the hypothesis that ECM degradation regulated by MMP-1 might facilitate rapid cellularization in tissue engineering. In the cell invasion test, collagenase of certain concentration (0.01 mg mL-1) could significantly promote the migration of smooth muscle cells (SMCs). Then electrospun poly(ε-caprolactone) (PCL) grafts were modified with collagenase through immobilization by hydrophobin (HFBI). Surface characterization of the material confirmed the successful immobilization of collagenase. The ingrowth of SMCs into the collagenase-modified membrane was more than that into the untreated membrane. Results of subcutaneous implantation in rats indicated that the modified graft was beneficial for vascularization by promoting capillary formation. The results showed that the collagenase modified grafts could enhance SMC migration and this strategy may be a promising and attractive method for cellularization and vascularization in tissue engineering.

18.
Sci China Life Sci ; 58(8): 765-72, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26014212

RESUMO

A new biomimetic material for artificial blood vessel with in situ catalytic generation of nitric oxide (NO) was prepared in this study. Organoselenium immobilized polyethyleneimine as NO donor catalyst and sodium alginate were alternately loaded onto the surface of electrospun polycaprolactone matrix via electrostatic layer-by-layer self-assembly. This material revealed significant NO generation when contacting NO donor S-nitrosoglutathione (GSNO). Adhesion and spreading of smooth muscle cells were inhibited on this material in the presence of GSNO, while proliferation of endothelial cells was promoted. In vitro platelet adhesion and arteriovenous shunt experiments demonstrated good antithrombotic properties of this material, with inhibited platelet activation and aggregation, and prevention of acute thrombosis. This study may provide a new method of improving cellular function and antithrombotic property of vascular grafts.


Assuntos
Materiais Biocompatíveis/química , Óxido Nítrico/metabolismo , Compostos Organosselênicos/química , S-Nitrosoglutationa/metabolismo , Células 3T3 , Alginatos/química , Animais , Materiais Biocompatíveis/farmacologia , Prótese Vascular , Catálise , Adesão Celular/efeitos dos fármacos , Células Cultivadas , Ácido Glucurônico/química , Ácidos Hexurônicos/química , Humanos , Camundongos , Microscopia Confocal , Microscopia Eletrônica de Varredura , Miócitos de Músculo Liso/efeitos dos fármacos , Ativação Plaquetária/efeitos dos fármacos , Adesividade Plaquetária/efeitos dos fármacos , Agregação Plaquetária/efeitos dos fármacos , Poliésteres/química , Polietilenoimina/química , Propriedades de Superfície , Trombose/prevenção & controle
19.
J Mater Chem B ; 3(47): 9212-9222, 2015 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-32263136

RESUMO

Thrombosis and neointimal hyperplasia are the main causes for the failure of small diameter vascular grafts, and a complete and functional endothelium is essential in preventing these problems. Therefore, grafts that could be endothelialized rapidly are highly desirable. This study constructed a vascular graft with catalytic nitric oxide (NO) generation and promoted endothelial cell (EC) adhesion for rapid in situ endothelialization, and examined the in vivo performance of an NO-generating vascular graft for the first time. A macroporous electrospun polycaprolactone (PCL) graft was prepared and modified via layer-by-layer self-assembly. Organoselenium immobilized polyethyleneimine was loaded onto the graft for in situ catalytic NO generation, while hyaluronic acid was grafted with an EC specific peptide Arg-Glu-Asp-Val and deposited to promote EC adhesion. This dual-modified material generated a strong and sustained flow of NO from S-nitrosoglutathione and significantly enhanced EC adhesion in vitro. In a co-culture experiment of ECs and smooth muscle cells (SMCs), this material promoted the adhesion of ECs and increased the EC/SMC ratio. After implantation in rats, the modified grafts showed a remarkably promoted endothelialization compared to PCL ones with an endothelium coverage of 89% versus 55% after 4 weeks, and the ECs on modified grafts were better organized in a pattern similar to that of the native vessel. The results indicated that the combination of catalytic NO generation and promoted EC adhesion proposed in this work may be a promising method for rapid endothelialization of small diameter vascular grafts.

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