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1.
Front Genet ; 12: 584886, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33613633

RESUMO

Heat shock protein 90 (HSP90) possesses critical functions in plant developmental control and defense reactions. The HSP90 gene family has been studied in various plant species. However, the HSP90 gene family in cucumber has not been characterized in detail. In this study, a total of six HSP90 genes were identified from the cucumber genome, which were distributed to five chromosomes. Phylogenetic analysis divided the cucumber HSP90 genes into two groups. The structural characteristics of cucumber HSP90 members in the same group were similar but varied among different groups. Synteny analysis showed that only one cucumber HSP90 gene, Csa1G569290, was conservative, which was not collinear with any HSP90 gene in Arabidopsis and rice. The other five cucumber HSP90 genes were collinear with five Arabidopsis HSP90 genes and six rice HSP90 genes. Only one pair of paralogous genes in the cucumber HSP90 gene family, namely one pair of tandem duplication genes (Csa1G569270/Csa1G569290), was detected. The promoter analysis showed that the promoters of cucumber HSP90 genes contained hormone, stress, and development-related cis-elements. Tissue-specific expression analysis revealed that only one cucumber HSP90 gene Csa3G183950 was highly expressed in tendril but low or not expressed in other tissues, while the other five HSP90 genes were expressed in all tissues. Furthermore, the expression levels of cucumber HSP90 genes were differentially induced by temperature and photoperiod, gibberellin (GA), downy mildew, and powdery mildew stimuli. Two cucumber HSP90 genes, Csa1G569270 and Csa1G569290, were both differentially expressed in response to abiotic and biotic stresses, which means that these two HSP90 genes play important roles in the process of cucumber growth and development. These findings improve our understanding of cucumber HSP90 family genes and provide preliminary information for further studies of cucumber HSP90 gene functions in plant growth and development.

2.
Ying Yong Sheng Tai Xue Bao ; 27(2): 519-24, 2016 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-27396126

RESUMO

The melon cultivar 'Yangjiaosu' was subjected to the treatment of 18 °C/12 °C (day/night) in an artificial climate chamber for 6 days, and the activities of nitrogen metabolism related enzymes [nitrate reductase (NR), glutamine synthetase (GS), glutamate synthase (GOGAT) and glutamate dehydrogenase (GDH) ] , the contents of total N, NO3(-)-N and NH4+-N as well as the osmotic adjustment substances of melon leaf were then determined. The results showed that, compared with the control, sub-low temperature treatment reduced the contents of total N, NO3(-)-N and the NR activity, but increased the content of NH4(+)-N, thereby leading to the growth inhibition of melon. Exogenous MT treatment significantly improved the activities of nitrogen metabolism related enzymes, especially the activities of GS and GOGAT, effectively reducing the content of NH4+-N. Moreover, MT treatment increased the contents of proline, soluble protein and soluble sugar, and alleviated the damage of sub-low temperature on the cell membrane by reducing the relative electrical conductivity and MDA content of melon leaves. In short, this work suggested that exogenous MT would enhance the sub-low temperature adaptability of melon by decreasing the leaf content of NH4-N, increasing the contents of osmotic adjustment substances and reducing the membrane lipid peroxidation levels.


Assuntos
Temperatura Baixa , Cucurbitaceae/fisiologia , Melatonina/farmacologia , Nitrogênio/metabolismo , Glutamato Desidrogenase/metabolismo , Glutamato Sintase/metabolismo , Glutamato-Amônia Ligase/metabolismo , Nitrato Redutase/metabolismo , Folhas de Planta , Plântula/fisiologia
3.
Ying Yong Sheng Tai Xue Bao ; 25(5): 1395-400, 2014 May.
Artigo em Chinês | MEDLINE | ID: mdl-25129941

RESUMO

The present study evaluated the effects of exogenous silicon on growth and physiological characteristics of hydroponically cultured cucumber seedlings under ammonium stress. The results showed that the growth, especially the aerial part growth of cucumber seedlings cultured with ammonium were significantly inhibited than those with nitrate, especially after treatment for 10 d, the aerial part fresh mass of cucumber seedlings were reduced 6.17 g per plant. The accumulation of reactive oxygen species (ROS) was also promoted in cucumber seedlings under ammonium, and the contents of O2*- and H2O2 were significantly increased in cucumber leaves. With the exogenous silicon treatment, the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX) were significantly improved, the ability to remove reactive oxygen species was enhanced, the contents of O2*- and H2O2 were significantly reduced in cucumber leaves, decreasing the reactive oxygen damage to the cell membrane, and the ratio of electrolyte leakage and the content of MDA in cucumber leaves. Also, with exogenous silicon treatment, the plasma membrane and activity of vacuolar membrane H(+)-ATP was significantly increased, transport capacity of intracellular proton was improved, and the level of ammonium in cucumber body was significantly reduced, thereby reducing the toxicity of ammonium. In conclusion, exogenous silicon could relieve ammonium stress, by increasing the antioxidant enzyme activity, H(+)-ATP activity, and decreasing the ammonium content in cucumber seedlings.


Assuntos
Compostos de Amônio/toxicidade , Cucumis sativus/fisiologia , Silício/farmacologia , Antioxidantes , Catalase , Cucumis sativus/efeitos dos fármacos , Peróxido de Hidrogênio , Peroxidases , Folhas de Planta , Espécies Reativas de Oxigênio , Plântula/efeitos dos fármacos , Plântula/fisiologia , Superóxido Dismutase
4.
Ying Yong Sheng Tai Xue Bao ; 20(11): 2685-90, 2009 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-20136001

RESUMO

By the method of hydroponic culture, this paper studied the alleviation effects of LaCl3 on the photosynthetic characteristics of cucumber seedlings under nitrate (140 mmol NO3(-) x L(-1)) stress, with the related mechanisms discussed. Under nitrate stress, the seedlings leaf chlorophyll and carotenoids contents decreased significantly, and the leaf Mg2+-ATPase, Ca2+-ATPase activities also decreased. On the 7th day of nitrate stress, the decrease of seedlings photosynthetic rate was mainly due to stomatal limitation; but on the 12th day of nitrate stress, the decrease was mainly due to no-stomatal limitation. Supplement with LaCl3 could make the cucumber seedlings keep relatively higher leaf Mg2+-ATPase and Ca2+-ATPase activities and chlorophyll and carotenoids contents, and applying 20 micromol x L(-1) of LaCl3 could increase the carotenoids content significantly. LaCl3 could also improve the leaf gas exchange, and alleviate the decrease of leaf Fv/Fm, PhiPSII, AQY, CE, and qp under nitrate stress, which helped the leaves making good use of light energy and maintaining higher CO2 assimilation capacity. An additional 20 micromol x L(-1) of LaCl3 could alleviate the nitrate stress on the photosynthesis of cucumber seedlings efficiently, but an additional 200 micromol x L(-1) of LaCl3 only had the alleviation effect at the initial period of nitrate stress. Our results could benefit to the improvement of greenhouse soil.


Assuntos
Cucumis sativus/fisiologia , Lantânio/farmacologia , Nitratos/toxicidade , Fotossíntese/fisiologia , Plântula/fisiologia , Clorofila/análise , Cucumis sativus/efeitos dos fármacos , Estresse Fisiológico
5.
Ying Yong Sheng Tai Xue Bao ; 19(8): 1854-9, 2008 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-18975769

RESUMO

By a solution culture experiment, cucumber seedlings were treated with different concentration (0, 25, 50 and 75 mmol x L(-1)) NaCl and NaHCO3, and their physiological responses were studied. The results showed that with increasing NaCl and NaHCO3 stress, the shoot and root growth, the leaf chlorophyll content and relative water content of the seedlings decreased dramatically, and the decrements were greater under NaHCO3 than under NaCl stress. The shoot Na content increased significantly with increasing NaCl and NaHCO, stress, while shoot K+ content was in adverse. At the same concentration of Na+ in culture solution, the addition of NaHCO3 induced a greater decrement of shoot K+ content, and thus, a lower shoot K+/Na+ ratio. Under NaHCO3 stress, the leaf electrolyte leakage rate and the leaf MDA, proline, and soluble sugar contents increased much greater, compared with those under NaCl stress. Under NaCl and NaHCO3 stress, the leaf superoxide dismutase (SOD), ascorbate peroxidase (APX) and dehydroascorbate reductase (DHAR) activities were induced significantly, while the leaf peroxidase (POD) activity was inhibited obviously.


Assuntos
Cucumis sativus/crescimento & desenvolvimento , Plântula/crescimento & desenvolvimento , Bicarbonato de Sódio/farmacologia , Cloreto de Sódio/farmacologia , Clorofila/metabolismo , Cucumis sativus/efeitos dos fármacos , Cucumis sativus/fisiologia , Peroxidase/metabolismo , Plântula/fisiologia , Superóxido Dismutase/metabolismo
6.
Ying Yong Sheng Tai Xue Bao ; 19(5): 976-80, 2008 May.
Artigo em Chinês | MEDLINE | ID: mdl-18655580

RESUMO

The study with water culture showed that nitrate stress had significant inhibitory effects on the plant growth, especially the shoot growth of cucumber seedlings. On the 7th day under nitrate stress, the shoot fresh mass per plant decreased by 12.77 g, leaf SOD, POD and CAT activities increased, while leaf APX, DHAR and GR activities decreased significantly, compared with the control. The addition of low concentration (0.05 mmol L(-1)) LaCl3 increased the shoot fresh mass per plant by 35%, enhanced the leaf thermally stable protein content and SOD, POD, CAT, APX, DHAR and GR activities, and decreased the leaf MDA content and electrolytic leakage, resulting in a definite alleviation of the inhibitory effects of nitrate. However, an addition of high concentration (0.5 mmol L(-1)) LaCl3 had no obvious alleviation effect. It was concluded that the addition of certain concentration LaCl3 could alleviate the nitrate stress on cucumber via increasing anti-oxidative enzymes activities and thermally stable protein content.


Assuntos
Cucumis sativus/efeitos dos fármacos , Lantânio/farmacologia , Nitratos/análise , Folhas de Planta/efeitos dos fármacos , Plântula/efeitos dos fármacos , Catalase/metabolismo , Cucumis sativus/crescimento & desenvolvimento , Cucumis sativus/metabolismo , Nitratos/metabolismo , Peroxidase/metabolismo , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Proteínas de Plantas/metabolismo , Plântula/crescimento & desenvolvimento , Plântula/metabolismo , Superóxido Dismutase/metabolismo
7.
Ying Yong Sheng Tai Xue Bao ; 17(3): 390-4, 2006 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-16724729

RESUMO

The study on the changes of physiological and biochemical properties of grafted eggplant seedling roots under low temperature stress and the relationships between these changes and cold tolerance showed that at the initial stage of treating with 10 degrees C (daytime) /3 degrees C (night), eggplant seedling roots were most impressible to low temperature, with the MDA content, chilling injury index, POD activity, proline content and soluble protein content increased significantly, and root respiration rate, SOD activity and CAT activity decreased rapidly. With the extending of low temperature stress, root respiration rate and osmotic adjustment reduced continually, while chilling injury index increased. After 3 days recovery, the respiration rate, osmotic adjustment and SOD activity were increased. Among the test materials, T2 (with Hiranasu as rootstock) had the best recovery capability, followed by T1 (with Taibyo as rootstock) and CK (Jinong 2000). Grafting with stronger cold tolerance rootstock could improve eggplant root activity, and thus, its cold resistance markedly.


Assuntos
Raízes de Plantas/crescimento & desenvolvimento , Plântula/crescimento & desenvolvimento , Solanum melongena/crescimento & desenvolvimento , Catalase/metabolismo , Folhas de Planta/enzimologia , Solanum melongena/enzimologia , Superóxido Dismutase/metabolismo , Temperatura
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