Assuntos
Leucemia Mieloide Aguda , Complexo de Proteínas Formadoras de Poros Nucleares , Humanos , Leucemia Mieloide Aguda/genética , Complexo de Proteínas Formadoras de Poros Nucleares/genética , Masculino , Proteínas de Ligação a Poli-ADP-Ribose/genética , Pálpebras/anormalidades , Rearranjo Gênico , Proteínas Oncogênicas/genética , Proteínas Cromossômicas não HistonaRESUMO
Apolipoprotein E (apoE) plays an important role in lipoprotein and cholesterol metabolism. An association between serum cholesterol and blood pressure has been suggested by epidemiological and experimental studies. But it is still not clear whether the apoE polymorphism plays a role in regulating blood pressure. The present study was undertaken to determine the association among apoE genotype, serum cholesterol and blood pressure in 303 healthy Japanese workers. Amplified fragments of DNA by the polymerase chain reaction were digested with HhaI and analyzed by 3% agarose-gel electrophoresis. Individuals with the apoE3/2 genotype had significantly lower levels of total cholesterol and systolic blood pressure than either the apoE3/3 individuals or the apoE3/4 + 4/4 individuals (P <0.05). The hypothesis that apoE indirectly influences systolic blood pressure through total serum cholesterol was supported by a covariance analysis of linear structural equations.
Assuntos
Apolipoproteínas E/genética , Pressão Sanguínea/genética , Colesterol/sangue , Adulto , Apolipoproteína E2 , Apolipoproteína E3 , Sequência de Bases , Fatores de Confusão Epidemiológicos , Primers do DNA , Feminino , Frequência do Gene , Genótipo , Humanos , Japão , Masculino , Pessoa de Meia-IdadeRESUMO
The aim of the present study was to investigate the trend for obesity and the relationship between Trp64Arg mutation in the beta3-adrenergic receptor gene and obesity-related phenotypes in Japanese workers. A total of 658 workers, 483 women and 175 men, responded to this survey. Genomic DNA was prepared from leukocytes, and DNA amplification by polymerase chain reaction was performed using 100 ng genomic DNA. Amplified fragments were digested with BstNI and analyzed by 3% agarose gel electrophoresis. The body mass index (BMI) increased from 21.1 +/- 2.6 to 22.0 +/- 3.0 kg/m2 for women and from 20.9 +/- 2.1 to 22.1 +/- 2.6 kg/m2 for men from 1982 to 1997. Body weight increased 63.3% for women and 67.4% for men over 15 years. The frequency of the Trp64Arg allele was 19.9%, and the frequency of heterozygote and homozygote carriers of the mutation was 32.9% and 3.4%, respectively. Changes in the BMI and body weight over 15 years from 1982 to 1997 did not differ significantly between subjects with or without the mutant allele in either women or men. The BMI was not significantly higher in subjects who were homozygous or heterozygous for the Trp64Arg mutation versus subjects who were homozygous for the normal allele. This mutation of the beta3-adrenergic receptor gene is not likely a major determinant of moderate obesity in the Japanese population.
Assuntos
Mutação/genética , Obesidade/genética , Receptores Adrenérgicos beta/genética , Adulto , Fatores Etários , Índice de Massa Corporal , Peso Corporal/genética , Feminino , Heterozigoto , Homozigoto , Humanos , Japão , Leucócitos/metabolismo , Masculino , Pessoa de Meia-Idade , Fenótipo , Reação em Cadeia da Polimerase , Polimorfismo Genético/genética , Receptores Adrenérgicos beta 3 , Fatores SexuaisRESUMO
The method of participatory action-research has been introduced in planning and implementing health and welfare projects for the elderly in Izumo City since 1993. Public health researchers have participated in the projects with other related administrative sectors, citizen and non-profit organizations. This study analyzes the establishment and implementation of health policies of Izumo from the following five aspects: policy-making, priority, education, intersectoral cooperation and community development. The participatory action-research method was useful for developing the ability of health-staff to work with citizens; for integrating community care and community development; for using community resources effectively for primary health care; and for developing community-based education of citizens, medical students and health staff. This method can be and should be adopted for strengthening the policy-making ability of public health researchers who learn through cooperation with citizens and non-profit organizations.
