RESUMO
The fruits of Paulownia catalpifolia Gong Tong are used as a Chinese folk herbal medicine for the treatment of enteritis, tonsillitis, bronchitis, and dysentery, etc. Our previous study has identified new C-geranylated flavanones with obvious anti-proliferative effects in lung cancer A549 cells. In the present study, a new C-geranylated flavone, paucatalinone C (1) and five known C-geranylated flavanones (2-6) were isolated. In addition, a total of 34 C-geranylated flavonoids were detected by HPLC-DAD-ESI-MS/MS coupling techniques from the CH2Cl2 extract of P. catalpifolia. Futhermore, anti-aging effects of isolated compounds were evaluated in vitro with premature senescent 2BS cells induced by H2O2. Phytochemical results indicated that P. catalpifolia was a natural resource of abundant C-geranylated flavonoids. Diplacone (3) and paucatalinone A (5) were the potent anti-aging agents in the premature senescent 2BS cells induced by H2O2 and the C-geranyl substituent may be an important factor because of its lipophilic character.
Assuntos
Envelhecimento/efeitos dos fármacos , Flavonoides/química , Flavonoides/farmacologia , Magnoliopsida/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Linhagem Celular , Cromatografia Líquida de Alta Pressão , Flavonoides/isolamento & purificação , Frutas/química , Humanos , Peróxido de Hidrogênio/toxicidade , Estrutura Molecular , Extratos Vegetais/isolamento & purificação , Espectrometria de Massas em TandemRESUMO
OBJECTIVE: To investigate the chemical constituents from the fruits of Paulownia tomentosa. METHODS: The compounds were isolated and purified by column chromatography and their structures were identified on the basis of physicochemical properties and spectral analyses. RESULTS: Seven compounds were isolated and their structures were identified as ursolic acid (1), sesamin(2),2α,3α, 19a-trihydroxyurs-12-en-28-oic acid(3), luteolin(4), tricin-7-O-ß-D-glucoside(5),3',4',5,7-tetrahydroxy-6-[7-hydroxy-3,7-dimethyl-2(E)-octenyl] flavanone(6) and stigmasterol(7). CONCLUSION: Compounds 3, 5 and 7 are isolated from Paulownia genus for the first time. Compound 2 is isolated from Paulownia tomentosa for the first time.
Assuntos
Frutas/química , Lamiales/química , Compostos Fitoquímicos/química , Flavanonas , Luteolina , Compostos Fitoquímicos/isolamento & purificação , Estigmasterol , Triterpenos , Ácido UrsólicoRESUMO
Three new geranylated flavanones, named as paucatalinone A (1), B (2), and isopaucatalinone B (3), were isolated from the fruits of Paulownia catalpifolia Gong Tong (Scrophulariaceae). Their structures were well determined by means of IR, MS, 1D and 2D NMR, and CD techniques. Paucatalinone A (1) is the first sample as a dimeric geranylated flavanone derivative isolated from natural products. Paucatalinone A (1) displayed good antiproliferative effects on human lung cancer cells A549 and resulted in a clear increase of the percentage of cells in G1 phase and a decrease in the percentage of cells in S and G2/M phases in comparison with control cells.
Assuntos
Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Neoplasias Pulmonares/tratamento farmacológico , Scrophulariaceae/química , Antineoplásicos Fitogênicos/isolamento & purificação , Linhagem Celular Tumoral/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais/métodos , Flavanonas/química , Flavanonas/isolamento & purificação , Flavanonas/farmacologia , Frutas/química , Fase G1/efeitos dos fármacos , Humanos , Neoplasias Pulmonares/patologia , Espectroscopia de Ressonância Magnética , Estrutura MolecularRESUMO
OBJECTIVE: To investigate the expression of TM4SF9 in the villi of early pregnancy, hydatidiform mole, invasive hydatidiform mole and chorionic carcinoma tissue. METHODS: Immunohistochemistry was used to detect the expression of TM4SF9 in normal villi of early pregnancy, hydatidiform mole, invasive hydatidiform mole and chorionic carcinoma tissues. RESULTS: TM4SF9 was expressed in the cytotroblasts but not in the syncytiotrophoblast of normal villi. The intensity of TM4SF9 expression increased in the order of normal villi, hydatidiform mole, invasive hydatidiform mole and chorionic carcinoma, with strong positivity rates of 0, 10%, 36.4% and 100%, respectively, showing significant differences between the samples (P<0.001). CONCLUSION: TM4SF9 expression in the trophoblasts may relate to their invasiveness and play an important role in the metastasis of trophoblastic tumor.
