LncRNA AC006064.4-201 serves as a novel molecular marker in alleviating cartilage senescence and protecting against osteoarthritis by destabilizing CDKN1B mRNA via interacting with PTBP1.

BACKGROUND: Osteoarthritis (OA) is the most prevalent age-related disease in the world. Chondrocytes undergo an age-dependent decline in their proliferation and synthetic capacity, which is the main cause of OA development. However, the intrinsic mechanism of chondrocyte senescence is still unclear. This study aimed to investigate the role of a novel long non-coding RNA (lncRNA), AC006064.4-201 in the regulation of chondrocyte senescence and OA progression and to elucidate the underlying molecular mechanisms. METHODS: The function of AC006064.4-201 in chondrocytes was assessed using western blotting, quantitative real-time polymerase chain reaction (qRT-PCR), immunofluorescence (IF) and ß-galactosidase staining. The interaction between AC006064.4-201 and polypyrimidine tract-binding protein 1 (PTBP1), as well as cyclin-dependent kinase inhibitor 1B (CDKN1B), was evaluated using RPD-MS, fluorescence in situ hybridization (FISH), RNA immunoprecipitation (RIP) and RNA pull-down assays. Mice models were used to investigate the role of AC006064.4-201 in post-traumatic and age-related OA in vivo. RESULTS: Our research revealed that AC006064.4-201 was downregulated in senescent and degenerated human cartilage, which could alleviate senescence and regulate metabolism in chondrocytes. Mechanically, AC006064.4-201 directly interacts with PTBP1 and blocks the binding between PTBP1 and CDKN1B mRNA, thereby destabilizing CDKN1B mRNA and decreasing the translation of CDKN1B. The in vivo experiments were consistent with the results of the in vitro experiments. CONCLUSIONS: The AC006064.4-201/PTBP1/CDKN1B axis plays an important role in OA development and provides new molecular markers for the early diagnosis and treatment of OA in the future. Schematic diagram of AC006064.4-201 mechanism. A schematic diagram of the mechanism underlying the effect of AC006064.4-201.

Ground-State Phase Diagram of a Spin-Orbital-Angular-Momentum Coupled Bose-Einstein Condensate.

By inducing a Raman transition using a pair of Gaussian and Laguerre-Gaussian laser beams, we realize a ^{87}Rb condensate whose orbital angular momentum (OAM) and its internal spin states are coupled. By varying the detuning and the coupling strength of the Raman transition, we experimentally map out the ground-state phase diagram of the system for the first time. The transitions between different phases feature a discontinuous jump of the OAM and the spin polarization, and hence are of first order. We demonstrate the hysteresis loop associated with such first-order phase transitions. The role of interatomic interaction is also elucidated. Our work paves the way to explore exotic quantum phases in the spin-orbital-angular-momentum coupled quantum gases.

Multiple side-band generation for two-frequency components injected into a tapered amplifier.

We have experimentally studied multiple side-band generation for two-frequency components injected into a tapered amplifier (TA) and demonstrated its effects on atomic laser cooling. A heterodyne frequency-beat measurement and a Fabry-Perot interferometer have been applied to analyze the side-band generation with different experimental parameters, such as frequency difference, injection laser power, and TA current. In laser-cooling potassium40 and potassium41 with hyperfine splitting of 1.3 GHz and 254 MHz, respectively, the side-band generation with a small frequency difference has a significant effect on the number of trapped atoms.