Identification and Validation of a Hypoxia and Glycolysis Prognostic Signatures in Lung Adenocarcinoma.

Background: Lung adenocarcinoma (LUAD) represents a prevalent subtype of non-small cell lung cancer with a complex molecular landscape. Dysregulated cellular energetics, notably the interplay between hypoxia and glycolysis, has emerged as a hallmark feature of LUAD tumorigenesis and progression. In this study, we aimed to identify hypoxia and glycolysis related gene signatures and construct a prognostic model to enhance the clinical management of LUAD. Methods: A gene signature associated with hypoxia and glycolysis was established within the The Cancer Genome Atlas (TCGA) cohort and subsequently validated in the GSE31210 cohort. Additionally, a nomogram was formulated to aid in predictive modeling. Subsequently, an evaluation of the tumor microenvironment and immune checkpoints expression levels was conducted to discern disparities between low risk and high risk groups. Lastly, an exploration for drugs with potential effectiveness was carried out. Results: Our analyses revealed a distinct hypoxia and glycolysis related gene signature consisting of 6 genes significantly associated with LUAD patient survival. Integration of these genes into the prognostic model demonstrated superior predictive accuracy for patient outcomes. Furthermore, we developed a user-friendly nomogram that effectively translates the model's prognostic information into a practical tool for clinical decision-making. Conclusion: This study elucidates the critical role of hypoxia and glycolysis related genes in LUAD and offers a novel prognostic model with promising clinical utility. This model has the potential to refine risk stratification and guide personalized therapeutic interventions, ultimately improving the prognosis of LUAD patients.

Porous silicon decorated with Au/TiO2 nanocomposites for efficient photoinduced enhanced Raman spectroscopy.

In this study, we investigated the potential of porous silicon (PSi) modified with Au/TiO2 nanocomposites (NCPs) as a substrate for photoinduced enhanced Raman spectroscopy (PIERS). One-step pulsed laser-induced photolysis (PLIP) was used to embed Au/TiO2 NCPs in the surface of PSi. Scanning electron microscopy revealed that adding TiO2 nanoparticles (NPs) during PLIP led to the formation of predominantly spherical Au NPs with a diameter of approximately 20 nm. Furthermore, modifying the PSi substrate with Au/TiO2 NCPs considerably enhanced the Raman signal of rhodamine 6G (R6G) after 4 h of ultraviolet (UV) irradiation. Real-time monitoring of the Raman signals of R6G at different concentrations under UV irradiation revealed that the amplitude of the signals increased with the irradiation time for R6G concentrations ranging from 10-3 M to 10-5 M. PSi substrates decorated with Au/TiO2 NCPs may be used to develop materials for PIERS applications.