RESUMO
BACKGROUND & OBJECTIVE: Transcription factors of human cancer cells can regulate the expression of many malignancy-related gene products,relate to poor differentiation,and high metastasis of cancer cells,and also inhibit apoptosis of cancer cells. This study was designed to investigate the nuclear factor-kappa B (NF-kappaB)DNA binding activity in human astrocytoma tissue,and its significance with human astrocytoma development. METHODS: The NF-kappaB DNA binding activities of 12 cases of normal brain tissue, and 37 cases of human astrocytoma tissue were detected by electrophoretic mobility shift assay (EMSA), and photo densitometry. RESULTS: The absorbency of NF-kappaB DNA binding activity in human astrocytoma tissue was 134.2+/-24.1, higher than that in normal brain tissue (97.5+/-1.9)(P< 0.05). The NF-kappaB DNA binding activity in multiform glioblastoma tissue was highest (106.8+/-7.4), that in anaplasia Astrocytoma was 123.2+/-10.1, in astroblastoma was 139.3+/-16.8,and in astrocytoma was 160.2+/-18.6 (P< 0.05). CONCLUSION: The NF-kappaB DNA binding activity correlates with malignancy degree of human astrocytoma.
