Anticonvulsant effects of ethanol stem bark extract of Lannea barteri (Anacardiaceae) in mice and chicks.

ETHNOPHARMACOLOGICAL RELEVANCE: Preparation of Lannea barteri is used in the treatment of epilepsy, gastritis, childhood convulsions among other uses in northern Nigeria for many years. The popularity of its efficacy is well established among the Traditional Medical Practitioners. AIM OF THE STUDY: The present study aimed at screening the ethanol stem bark extract of Lannea barteri for possible anticonvulsant action. MATERIALS AND METHOD: Anticonvulsant screening was carried out using pentylenetetrazole (PTZ), strychnine (STN) and picrotoxin (PTC) induced seizures in mice while Maximal electroshock (MES) test was carried out in day old chicks. Preliminary phytochemical screening of the extract was performed on the extract. The intraperitoneal median lethal dose (LD50) was carried out in mice. RESULTS: The intraperitoneal (i.p.) LD50 of the extract was estimated to be 567.70 mg/kg in mice. Lannea barteri (160 mg/kg) significantly (p ≤ 0.05) delayed the mean onset of seizures induced by PTZ when compared with normal saline treated group. Similarly, the extract at 160 mg/kg significantly (p ≤ 0.05) prolonged the latency of convulsion induced by STN. Lannea barteri (40 mg/kg) significantly (p ≤ 0.05) delayed the mean onset of seizures induced by picrotoxin in mice. The extracts at all the doses tested showed no observable effect in decreasing the mean recovery time of convulsed chicks in MEST. Flavonoids, alkaloids, tannins, saponins and glycosides were found present in the stem bark extract. CONCLUSION: Our findings revealed that the ethanol stem bark extract of Lannea barteri contained bioactive constituents that may be useful in the management of petit mal epilepsy and supports the ethnomedical claim for the use of its stem bark in the management of epilepsy.

Seroprevalence of cytomegalovirus among pregnant women attending Murtala Mohammed Specialist Hospital Kano, Nigeria.

BACKGROUND: Primary Cytomegalovirus (CMV) infection during pregnancy is a frequent and serious threat to the fetus. As there is no vaccine alternative measures are needed to prevent congenital CMV infection. OBJECTIVE: This study determined CMV Immunoglobulin G (IgG) antibody among pregnant women in order to ascertain the immune status of mothers to guide policy makers. METHODS: A semi-structured questionnaire was initially administered to obtain information on demographic details, stage of pregnancy and risk factors. Blood was collected by venipuncture from 180 women attending the antenatal clinic in Murtala Mohammed Specialist Hospital Kano, Kano State, Nigeria. Sera samples were screened using CMV IgG ELISA kit (Dialab, Austria). RESULTS: Out of 180 pregnant women, 164 (91.1%) were seropositive. Based on stages of pregnancy 6/6(100%), 52/60(86.7%) and 106/114(93.0%) were seropositive among women in the first, second and third trimesters respectively. CONCLUSION: Seroprevalence of pregnant women to CMV Ig G is high, hence the need for CMV - IgM screening to know the extent of active infection. There is also need for public enlightenment on the methods of transmission, effective prevention and control strategies.

The efficacy of 10gram intramuscular loading dose of MgSO(4) in severe preeclampsia/ eclampsia at a tertiary referral centre in Northwest Nigeria.

AIMS AND OBJECTIVES: The efficacy of 10g intramuscular loading dose of magnesium sulphate in women with severe preeclampsia/eclampsia was assessed at a tertiary health centre for potential use at primary health level. SUBJECTS AND METHODS: Intramuscular 10g loading dose and 14g loading dose of Pritchard were compared in women with severe preeclampsia/eclampsia. Primary outcome measures were the occurrence of fits in women with severe preeclampsia, further fits in those with eclampsia and maternal death. Other outcome measures were mode of delivery and severe birth asphyxia at 5 minutes of life. RESULTS: One hundred and three women were enrolled; 54 and 49 women had 10g and 14g loading dose respectively. No significant convulsions (p= 0.1424) occurred in women with severe preeclampsia who had 10g intramuscular loading dose and repeat convulsion was averted in 93% of women with eclampsia. 10g loading dose did not increase the likelihood of caesarean section in women with preeclampsia (p=0.2832) or eclampsia (p=0.9112). The mean Apgar score at 5 minutes of life of neonates whose mothers had 10g and 14g loading dose for preeclampsia was 8 and 8.46 respectively, and 8.9 and 8.8 respectively for eclampsia. There was no statistically significant difference in maternal death between the two groups for severe preeclampsia (p= 0.2020) and eclampsia (p=0.3496). CONCLUSION: This study suggests a potential use of intramuscular 10 gram loading dose of MgSO at the primary health care level in Nigeria.

The efficacy of intramuscular loading dose of MgSO4 in severe pre-eclampsia/ eclampsia at a tertiary referral centre in Northwest Nigeria.

AIMS AND OBJECTIVES: The efficacy of 10g intramuscular loading dose of magnesium sulphate in women with severe preeclampsia/eclampsia was assessed at a tertiary health centre for potential use at primary health level. SUBJECTS AND METHODS: Intramuscular 10g loading dose and 14g loading dose of Pritchard were compared in women with severe preeclampsia/eclampsia. Primary outcome measures were the occurrence of fits in women with severe preeclampsia, further fits in those with eclampsia and maternal death. Other outcome measures were mode of delivery and severe birth asphyxia at 5 minutes of life. RESULTS: One hundred and three women were enrolled; 54 and 49 women had 10g and 14g loading dose respectively. No significant convulsions (p= 0.1424) occurred in women with severe preeclampsia who had 10g intramuscular loading dose and repeat convulsion was averted in 93% of women with eclampsia. 10g loading dose did not increase the likelihood of caesarean section in women with preeclampsia (p=0.2832) or eclampsia (p=0.9112). The mean Apgar score at 5 minutes of life of neonates whose mothers had 10g and 14g loading dose for preeclampsia was 8 and 8.46 respectively, and 8.9 and 8.8 respectively for eclampsia. There was no statistically significant difference in maternal death between the two groups for severe preeclampsia (p= 0.2020) and eclampsia (p=0.3496). CONCLUSION: This study suggests a potential use of intramuscular 10 gram loading dose of MgSO4 at the primary health care level in Nigeria.

Vesico-vaginal fistula in a patient with Meyer-Rokitansky-Kuster-Hauser syndrome.

This was a case of Gishiri cut in a patient with Meyer-Rokitansky-Kuster-Hauser syndrome resulting in a vesico-vaginal fistula and urethral loss. This followed an attempt to enlarge and lengthen the vagina to enhance penile penetration. Few cases of MRKH syndrome presenting with complications after an attempt at treatment by traditional birth attendants have been reported. This report is particularly of essence as most urinary fistulae in Nigeria are obstetric fistulae following prolonged obstructed labour.

Menstrual pain and associated factors amongst undergraduates of Ambrose Alli University Ekpoma, Edo State, Nigeria.

BACKGROUND: We determined the prevalence of dysmenorrhoea and associated factors amongst undergraduates in a Nigerian university. Information on dysmenorrhoea amongst Nigerian populace is limited as it is seen as a norm and hence unreported. This is in spite of its consequences on women's socio-economic lives. METHOD: This was a prospective questionnaire-based study. Cluster sampling technique was used and tests of statistical significance were done using Yates corrected Chi square. RESULTS: The prevalence of dysmenorrhoea in this study was 76.3%. The mean age at menarche was 13.8 years. Dysmenorrhoea occurred at menarche in 36.9% respondents. Primary and secondary dysmenorrhoea was reported by 40.6% and 16.3% respondents respectively. Fifty one point nine per cent (51.9%) had painful expectation of dysmenorrhoea. Having a sister with dysmenorrhoea did not have a statistically significant influence on expectation of dysmenorrhoea (CI 95%; P value: 0.76). Normal activity was affected by dysmenorrhoea in 35% of respondents while 68% of those with dysmenorrhoea did not seek help. Hospital admission solely for dysmenorrhoea was reported by 6.9% of respondents. Commonly associated symptoms with dysmenorrhoea were mood changes (59.4%) and pimples (53.1%). CONCLUSION: The high proportion of women not seeking help for dysmenorrhoea in this study may reflect the attitude of the larger society to the condition. Education to improve women's knowledge of and attitude to dysmenorrhoea is recommended.

Menstrual Pain and Associated Factors amongst Undergraduates of Ambrose Alli University Ekpoma; Edo State; Nigeria

Background: We determined the prevalence of dysmenorrhoea and associated factors amongst undergraduates in a Nigerian university. Information on dysmenorrhoea amongst Nigerian populace is limited as it is seen as a norm and hence unreported. This is in spite of its consequences on women's socio-economic lives. Method: This was a prospective questionnaire-based study. Cluster sampling technique was used and tests of statistical significance were done using Yates corrected Chi square. Results: The prevalence of dysmenorrhoea in this study was 76.3. The mean age at menarche was 13.8 years. Dysmenorrhoea occurred at menarche in 36.9respondents. Primary and secondary dysmenorrhoea was reported by 40.6and 16.3respondents respectively. Fifty one point nine per cent (51.9) had painful expectation of dysmenorrhoea. Having a sister with dysmenorrhoea did not have a statistically significant influence on expectation of dysmenorrhoea (CI 95; P value: 0.76). Normal activity was affected by dysmenorrhoea in 35of respondents while 68of those with dysmenorrhoea did not seek help. Hospital admission solely for dysmenorrhoea was reported by 6.9of respondents. Commonly associated symptoms with dysmenorrhoea were mood changes (59.4) and pimples (53.1). Conclusion: The high proportion of women not seeking help for dysmenorrhoea in this study may reflect the attitude of the larger society to the condition. Education to improve women's knowledge of and attitude to dysmenorrhoea is recommended