Association of metabolic syndrome components with the genotypes of the С825Т polymorphism in the g protein ß3-subunit gene (GNB3).

OBJECTIVE: Introduction: All components of the metabolic syndrome (MS) are the risk factors for the cardiovascular diseases, and their combination a great deal accelerates and complicates development of the diseases. Phenotypic expression of MS depends on the interaction of genetic and environmental factors. The aim: The aim is to study the association of metabolic syndrome components with the genotypes of the C825T polymorphism in the GNB3 gene, which allows predicting the risks and determining individual lifestyle and treatment program for the future. PATIENTS AND METHODS: Materials and methods: The patients were analyzed for anthropomorphic data and abdominal obesity. Presence of MS criteria was assessed in the patients in accordance with the consensus of International Diabetes Federation (2009). Hypertension diagnosis is based on the recommendations of adapted Clinical Practice Guidelines "Arterial Hypertension" (2012). The study of the С825Т polymorphism in the G protein ß3-subunit gene was conducted at molecular-genetic research laboratory of Sumy State University with subsequent analysis of restriction fragment length polymorphism. The data were statistically processed using SPSS Statistics 21.0 program on PC. RESULTS: Results: T-allele carriers had 1.6 times higher risk of obesity than C-allele homozygotes ( р=0.034). Т825Т genotype carriers were 2.7 times higher risk of hypertension compared the carriers of С825С genotype of the С825Т polymorphism of the GNB3 gene ( р = 0.002). The risk of increased LDL cholesterol level in the minor allele carriers is 3.9 times higher than that in the major allele carriers. ( р = 0.002). CONCLUSION: Conclusions: The results of our study concerning the association of the minor allele and T825 + C825T genotypes with the risk of components of the MS.

Association of matrix Gla protein gene allelic polymorphisms (G(-7)→A, T(-138)→C and Thr(83)→Ala) with acute coronary syndrome in the Ukrainian population.

BACKGROUND: Several allelic variants of matrix γ-carboxyglutamic acid protein (MGP) can differentially affect the development of certain forms of ischemic heart disease depending on specific characteristics of each population. OBJECTIVE: To study the distribution of allelic variants of MGP promoter T(-138)→C (rs1800802) and G(-7)→A (rs1800801), and Thr(83)→Ala exon 4 (rs4236) polymorphisms in a Ukrainian population of patients with acute coronary syndrome (ACS). METHODS: Polymerase chain reaction and restriction fragment length polymorphism (RFLP) analysis were used to detect the above-mentioned variants of the MGP gene in 115 patients with ACS and in 140 essentially healthy individuals. RESULTS: The distribution of homozygous carriers of a major allelic variant, and heterozygous and homozygous minor allele variants of the T(-138)→C MGP promoter polymorphism in patients with ACS were 59.8%, 32.7% and 7.5%, respectively. The corresponding distributions of variants in the control group were 54.0%, 41.0% and 5.0%, respectively (P>0.05 [χ(2) test]). With respect to the G(-7)→A polymorphism, the respective distributions were 42.1%, 45.6% and 12.3%, compared with 50.7%, 45.0% and 4.3% in the control group, respectively (P<0.05). Finally, the respective distributions according to the Thr(83)→Ala exon 4 polymorphism were 42.6%, 43.5% and 13.9%, respectively, compared with 45.3%, 43.0% and 11.7% in the control group. Using logistic regression analysis, it was estimated that the A/A genotype (G(-7)→A polymorphism) was significantly (P=0.02) associated with ACS (OR 4.302 [95% CI 1.262 to 14.673]). CONCLUSION: The allelic A/A promoter variant of MGP G(-7)→A polymorphism can be considered a risk factor for ACS in the Ukrainian population.