Ultrasonographic features of nonvascular complications of hyaluronic acid fillers: a retrospective study at a reference center for dermatologic ultrasonography.

Hyaluronic acid filler injections have been associated with early, temporary, and delayed-onset complications. High-resolution ultrasound with Doppler analysis has been increasingly used to detect and identify such complications. We comprehensively describe the most common ultrasonographic findings of nonvascular complications associated with hyaluronic acid filler injections. This retrospective, cross-sectional, observational study was conducted at a reference center for dermatologic ultrasound in Bogotá, Colombia. Ultrasound reports documented the ultrasonographic findings of nonvascular complications of hyaluronic acid filler injections. Fifty-two complications were documented in a cohort of 50 patients (women, 88%). The infraorbital region was the most common site affected (23%), followed by the nasolabial region (22%). The Tyndall effect was the most common complication (25% of all), followed by changes in rheology (21%) and pseudosarcoidal (foreign body granuloma) reaction (15%). The Tyndall effect stood out for its distinctive ultrasonographic characteristics. We discuss the ultrasonographic findings and pathogenesis of other complications, including filler migration, early hypersensitivity, aseptic abscess, overcorrection, and filler material interaction. The clinical presentation of hyaluronic acid filler complications can be confusing, delaying timely diagnosis and treatment. High-resolution ultrasound with Doppler analysis is a valuable tool for avoiding unnecessary treatments and ensuring timely diagnosis and treatment.

Cognitive Keys in Psychophysical Estimation of Chemosensory Perception in University Students.

Psychophysical methods allow us to measure the relationship between stimuli and sensory perception. Of these, Detection Threshold (DT) allows us to know the minimum concentration to produce taste identification. Given this, we wonder whether, for example, wine tasting experts are more capable of perceiving their sensory properties than other people, or whether they can distinguish them because they are better able to "describe" them. To verify this, this study analyses the influence of having prior knowledge of the name astringency and, failing that, to detect it and distinguish it between the four basic tastes. One-hundred-and-sixty-two university students with an average age of 19.43 (SD = 2.55) years were assigned to three experimental conditions: an experimental group (G.2) without previous knowledge of the name astringency and with alimentary satiety, and two control groups, both with previous knowledge of the name, these being G.1, with satiety, and G.3, with hunger. DT was collected for the four basic tastes and astringencies. Results showed significant differences in the identification of astringency, being the least identified experimental group with respect to the control groups. It is striking that G.2, without prior knowledge of the name, identified astringency as a bitter taste in most cases. This supports our hypothesis of the importance of attending to linguistic cognitive processes when psychophysically estimating taste in humans.

Microencapsulated pomegranate peel extract induces mitochondrial complex IV activity and prevents mitochondrial cristae alteration in brown adipose tissue in mice fed on a high-fat diet.

Pomegranate peel is an agro-industrial residue obtained after fruit processing with high total polyphenol (TP) content, making it an attractive by-product for its reuse. Pomegranate peel extract (PPE) and its bioactive compounds have shown positive effects on obesity models. Effects on favouring mitochondrial biogenesis and function have also been described. However, once phenolic compounds are extracted, their stability can be affected by diverse factors. Microencapsulation could improve PPE stability, allowing its incorporation into functional foods. Nevertheless, studies on the potential biological effects of PPE microparticles (MPPE) in obesity models are lacking. This study aims to evaluate the effect of MPPE on brown adipose tissue (BAT) mitochondrial structure and function and metabolic alterations related to obesity in mice fed a high-fat diet (HFD). PPE was microencapsulated by spray drying using inulin (IN) as a wall material and physically-chemically characterised. Eight-week-old male C57BL/6J mice (n 40) were randomly distributed into five groups: control diet (CD), HFD, HFD + IN, HFD + PPE (50 mg/kg per d TP) and HFD + MPPE (50 mg/kg per d TP), for 14 weeks. A glucose tolerance test and indirect calorimetry were conducted. Blood and adipose tissue samples were obtained. MPPE supplementation prevented HFD-induced body weight gain (P < 0·001), fasting glycaemia (P = 0·007) and total cholesterol rise (P = 0·001). MPPE resulted in higher BAT mitochondrial complex IV activity (P = 0·03) and prevented HFD-induced mitochondrial cristae alteration (P = 0·02). In conclusion, MPPE prevented HFD-induced excessive body weight gain and associated metabolic disturbances, potentially by activating complex IV activity and preserving mitochondrial cristae structure in BAT in mice fed with a HFD.

Automedicación con fármacos modificadores de pH gástrico: Estudio sistemático y metaanalítico

Objetivo: Determinar de la prevalencia de automedicación con fármacos modificadores de pH gástrico en América Latina. Material y métodos: Se realizó una búsqueda sistemática utilizando Google Scholar, PubMed, SciELO y repositorios de tesis iodo de enero de 2000 hasta agosto de 2021. Solo se incluyó estudios observacionales que contenían datos sobre automedicación y que contenían datos suficientes para calcular la prevalencia de automedicación, ya sea con número de pacientes o número de medicamentos. Se aplicó un modelo de efectos aleatorios para la construcción del diagrama de árbol en base al número de pacientes o número de medicamentos, con un intervalo de confianza (IC) del 95%. Resultados: Se incluyó 31 estudios, con un número total de 9 300 individuos encuestados y provenientes de 9 países (Argentina, Chile, Colombia, Ecuador, El Salvador, Honduras, México, Perú y Venezuela). La media de edad de los encuestados fue de 26,69 ± 11,15 años. Los estudios se realizaron en la población general, estudiantes universitarios y profesionales. La prevalencia combinada de automedicación con fármacos modificadores de pH gástrico fue del 13 % (IC de 95%: 8% a 18%, I =98%). Asimismo, los tres países con la mayor prevalencia de automedicación con fármacos modificadores de pH gástrico fueron: Colombia (53%), El Salvador (45%) y Perú (34%) Conclusión: La automedicación con necesario más estudios sobre la prevalencia y los factores asociados a este fenómeno.

Efecto de la extracción del cristalino en la presión intraocular de pacientes con glaucoma por cierre angular primario / Effect of crystalline lens extraction on intraocular pressure in patients with primary angle closure glaucoma

Objetivo: Describir el efecto de la extracción del cristalino transparente en la presión intraocular de pacientes con glaucoma por cierre angular primario. Métodos: Se realizó un estudio descriptivo longitudinal prospectivo en la provincia de Pinar del Río, entre enero del año 2013 y diciembre de 2019, que incluyó a 54 ojos de 54 pacientes con glaucoma por cierre angular primario y cristalino transparente, tratados con facoaspiración. El análisis estadístico se realizó a través del programa SPSS. Resultados: Hubo diferencias muy significativas (p< 0,001) en los valores de presión intraocular media entre el preoperatorio y el posoperatorio. El número de medicamentos no mostró diferencias significativas (p= 0,317) y el porcentaje de reducción promedio de la presión intraocular fue de 33,9 ± 4,3. Al final del período de seguimiento el 68,5 por ciento manifestó valores de 18 mmHg o más. El estadio glaucomatoso, la existencia o no de sinequias anteriores periféricas, el valor medio de la presión intraocular preoperatoria y del número de medicamentos mostraron diferencias significativas (p= 0,006; p= 0,001; p= 0,001 y p= 0,045 respectivamente) entre las categorías de control de la presión intraocular. Conclusión: La extracción del cristalino transparente reduce la presión intraocular en pacientes con glaucoma por cierre angular primario, lo que se corrobora a mediano plazo; aunque la mayoría de los ojos tratados no alcanzan un control total y mantienen el número de medicamentos sin variación(AU)

Objective: Describe the effect of clear lens extraction on intraocular pressure in patients with primary angle closure glaucoma. Methods: A prospective longitudinal descriptive study was conducted in the Province of Pinar del Río from January 2013 to December 2019. The study included 54 eyes of 54 patients with primary angle closure glaucoma and clear lens treated with phacoaspiration. Statistical analysis was based on SPSS software. Results: Very significant differences were found (p< 0.001) between preoperative and postoperative intraocular pressure values. Significant differences were not observed in the number of drugs indicated (p= 0.317). Average intraocular pressure reduction percentage was 33.9 ± 4.3. By the end of the follow-up period, 68.5 percent displayed values of 18 mmHg or higher. Significant differences were found between the intraocular pressure control categories with respect to the stage of the disease, the existence or non-existence of previous peripheral synechiae, the mean preoperative intraocular pressure value, and the number of drugs (p= 0.006; p= 0.001; p= 0.001 and p= 0.045, respectively). Conclusion: Clear lens extraction reduces intraocular pressure in patients with primary angle closure glaucoma, a fact corroborated in the medium term, though most of the eyes treated do not achieve total control and the number of drugs remains unaltered(AU)

Psychopathology, Body Image and Quality of Life in Female Children and Adolescents With Anorexia Nervosa: A Pilot Study on the Acceptability of a Pilates Program.

Background: Anorexia nervosa (AN) is a psychiatric illness that without early effective treatment becomes chronic with high physical, psychological and social morbidity and high mortality. Pilates exercises can improve quality of life and increase body awareness in different clinical and healthy populations. The aim of this pilot study was to examine the acceptability of a Pilates program in a sample of female children and adolescents with AN by evaluating the psychopathological status, alterations in the perception of body image and health-related quality of life after 10 weeks. Methods: A total of 12 female patients (age: 14.6 ± 1.7 years) completed the 10-week Pilates program. Psychopathology (EDI-3), body image disturbance (CDRS) and quality of life (KIDSCREEN-27) were evaluated before and after the intervention. A satisfaction questionnaire was also provided. Results: Regarding psychopathology, although there were standardized reductions in seven parameters of those that form EDI-3, none of them reached significance. In relation to body image, significant, moderately standardized and substantial decreases were observed in the body dissatisfaction (p = 0.046, Cohen's d = -0.69). There were significant, large standardized and substantial increases in physical well-being (p = 0.008, Cohen's d = 1.37) and significant, moderately standardized and substantial decreases in autonomy and parent relation (p = 0.021, Cohen's d = -0.60). Satisfaction data was positive. Conclusion: A Pilates program could help to improve perceived health outcomes by decreasing body dissatisfaction and increasing physical well-being in female children and adolescents with AN, so Pilates seems to be a beneficial complementary treatment in children and adolescents with AN. These findings from our pilot study are encouraging for future research with a substantially larger sample size, representing the first phase of a longer process.

Pulmonary Sequestration Associated with Actinomycosis: A Case Report.

Background: Bronchopulmonary sequestration is a rare congenital malformation of the lower respiratory tract; it consists of a nonfunctioning mass of lung tissue that is irrigated by an anomalous systemic artery. The association with Actinomyces superinfection has not been well established. Methods: We present the case of a 35-year-old woman with a history of recurrent episodes of pneumonia. Based on radiological and histopathological examination, she was diagnosed with intralobar bronchopulmonary sequestration associated with Actinomyces infection. Promoting clinical suspicion is essential to diagnose pulmonary actinomycosis in patients with recurrent pneumonia, to improve early recognition and timely management.

Factores que influyen en la progresión del cierre angular primario posterior a iridotomía periférica láser / Factors influencing the progression of primary angular closure following Laser peripheral iridotomy

RESUMEN Introducción: los efectos de la iridotomía periférica láser están demostrados, sin embargo, no siempre logra controlar la presión intraocular ni la progresión de la enfermedad por cierre angular primario. Objetivo: analizar los factores que influyen en la progresión de la enfermedad por cierre angular primario de pacientes pinareños tratados con iridotomía periférica láser. Método: se realizó un estudio analítico de cohorte retrospectivo en el servicio de Oftalmología del Hospital General Docente Abel Santamaría, de Pinar del Río, durante el año 2019. El universo estuvo constituido por pacientes con diagnóstico de enfermedad por cierre angular primario tratados con iridotomía periférica láser. La muestra final quedó integrada por 223 ojos de 123 pacientes. El análisis estadístico se realizó con el programa SPSS. Resultados: el 20,6 % de la muestra experimentó progresión de la enfermedad, lo que se relacionó de forma significativa con la forma clínica (p<0,001), la edad (p=0,012), la amplitud de la cámara anterior (p<0,001), el cierre angular residual (p<0,001), la presión intraocular (p<0,001) y la medicación hipotensora (p<0,001). No arrojó diferencias significativas el sexo (p=0,427), el color de la piel (p=0,741) y la longitud axial (p=0,549). Conclusión: los factores que influyen en la progresión de la enfermedad por cierre angular primario de los pacientes pinareños tratados con iridotomía periférica láser estudiados fueron la forma clínica, menor amplitud de la cámara anterior, presencia de cierre angular residual y presión intraocular superior a 18 mmHg con uso de mayor número de colirios hipotensores oculares.

ABSTRACT Introduction: the effects of Laser peripheral iridotomy have been demonstrated; however it does not always manage to control intraocular pressure or the progression of the disease by primary angular closure. Objective: to analyze the factors influencing the disease progression by primary angular closure in Pinar del Rio patients treated with Laser peripheral iridotomy. Methods: a retrospective analytical cohort study was carried out in the Ophthalmology Service at Abel Santamaria Cuadrado General Teaching Hospital in Pinar del Río, during 2019. The target group comprised the patients diagnosed with primary angular closure disease treated with Laser peripheral iridotomy and the final sample consisted of 223 eyes from 123 patients. The statistical analysis was performed with the SPSS program. Results: of the sample (20,6 %) experienced disease progression, which was significantly related to the clinical form (p<0,001), age (p=0,012), anterior chamber amplitude (p<0,001), residual angular closure (p<0,001), intraocular pressure (p<0,001) and hypotensive medication (p<0,001). Sex (p=0,427), skin color (p=0,741) and axial length (p=0,549) did not show significant differences. Conclusions: factors influencing on the progression of the disease by primary angular closure in Pinar del Rio patients who were treated with Laser peripheral iridotomy were: the clinical form, lower anterior chamber amplitude, presence of residual angular closure and intraocular pressure higher than 18 mmHg with the use of more ocular hypotensive eye drops.

Iowa Gambling Task and Distortion in Perception of Body Image Among Adolescent Women With Eating Disorders.

The Iowa gambling task (IGT) is an instrument for the neuropsychological evaluation of cognitive and emotional decision making (DM) processes that was created to test the somatic marker hypothesis (SMH) described by Damasio in 1994. It was initially applied to patients with frontal lobe lesions due to its association with executive functions but was subsequently used on patients with a variety of disorders. Although the DM process is inherently perceptual, few studies have applied the IGT to examine DM processes in patients with eating disorders (EDs), and even fewer have associated the IGT to the perceptual distortion of body image (PDBI) in this population. People diagnosed with ED exhibit heightened control over their somatic responses-for example, they can delay digestion for hours-and DM may be affected in this condition. This study compares the performance of two samples of adolescent women-hospitalized patients with ED, and healthy controls with similar demographic characteristics-on the IGT using body image as a possible factor in the SMH. Seventy-four women with a mean age of 14.97 years (SD = 2.347) participated. To analyze their body self-image, we used the figure-rating scale and compared the results with their body mass index (BMI). Correlations between indices of the IGT and distortion in body image were then explored. The results revealed significant differences between the groups in terms of evolving performance on the partial IGT. Patients with ED performed worse than their healthy counterparts in the last 40 trials and exhibited greater distortions in their body image, especially in terms of overestimation. Indices of these distortions were negatively correlated with the total IGT. These results are compatible with the SMH because they suggest that patients with ED evinced blindness with regard to the future, as described by their authors. In addition, a negative correlation was found between the IGT and PDBI, showing that a more distorted body image was associated with lower IGT, that is, more disadvantageous or riskier decisions were made by the subjects with more distortion.

Consideraciones quirúrgicas de la facoemulsificación del cristalino en pacientes con cierre angular primario / Surgical considerations of phacoemulsification of the lens in patients with primary angular closure

RESUMEN Introducción: la cirugía del cristalino se considera uno de los métodos quirúrgicos más seguros a nivel mundial. Objetivo: describir las consideraciones quirúrgicas a tener en cuenta para lograr el éxito de la facoemulsificación del cristalino en pacientes con cierre angular primario en el Hospital General Docente "Abel Santamaría Cuadrado" de Pinar del Río. Métodos: se realizó una búsqueda de los principales artículos científicos de los últimos años, así como de la literatura impresa que incluye el tema, siendo seleccionados los contenidos más relevantes para la confección del informe final. Desarrollo: la extracción del cristalino constituye un reto quirúrgico en pacientes con cierre angular primario por las peculiaridades anatómicas, variaciones de la presión intraocular y alteraciones del segmento anterior asociadas. Conclusiones: un detallado examen preoperatorio, una adecuada técnica quirúrgica realizada por un cirujano hábil puede reducir el índice de complicaciones y lograr total éxito.

ABSTRACT Introduction: crystalline lens surgery is considered one of the safest surgical methods in the world. Objective: to describe the surgical considerations to be taken into account to achieve the success of phacoemulsification of the crystalline lens in patients with primary angular closure at Abel Santamaría Cuadrado General Teaching Hospital in Pinar del Río. Methods: literature searching was conducted concerning the main scientific articles of the last years, as well as the printed literature that includes the topic, choosing the most relevant contents for the writing of the final report. Development: the removal of the crystalline constitutes a surgical challenge in patients with primary angular closure due to anatomical peculiarities, intraocular pressure variations and associated alterations of the anterior segment. Conclusions: a detailed preoperative examination, and the adequate surgical technique performed by a skilled surgeon can reduce the rate of complications and achieve total success.

Epidemiología del cierre angular primario en Pinar del Río / Epidemiology of primary angular closure in Pinar del Río

RESUMEN Introducción: la clínica del cierre angular primario puede variar desde una sospecha de la enfermedad hasta estadios avanzados del daño glaucomatoso, lo que puede provocar ceguera. Objetivo: describir la epidemiología del cierre angular primario en pacientes pinareños, una vez identificadas las variables sociodemográficas y oculares que determinan su forma clínica. Métodos: se realizó un estudio descriptivo transversal en Pinar del Río, en los años 2013 y 2017, el universo estuvo constituidos por 293 casos nuevos con diagnóstico de cierre angular primario en cualquiera de sus formas clínicas. El análisis estadístico se realizó con el programa SPSS. Resultados: la edad media fue 57,69 ± 7,35 años; predominaron las mujeres (89,5 %) y el 77,2 % evidenció una situación de estrés. Los valores promedio de longitud axial y amplitud de cámara anterior fueron 21,89 ± 0,49 mm y 2,65 ± 0,27 mm. El 87,03 % de los ojos mostró ángulo estrecho. La media de la presión intraocular basal fue de 23,86 ± 5,81. Al analizar las variables sociodemográficas y oculares en relación a la forma clínica, se encontró que los factores que la determinan fueron: edad (p<0,001), amplitud angular (p<0,001), sinequias anteriores periféricas (p<0,001) y presión intraocular (p<0,001). El análisis de regresión lineal confirmó estos resultados. Conclusión: el cierre angular primario es frecuente en mujeres de mediana edad sometidas a estrés; con ojos pequeños, ángulo camerular y cámara anterior estrecha. La edad, amplitud angular, sinequias anteriores periféricas y presión intraocular basal, determinan la forma clínica de la enfermedad.

ABSTRACT Introduction: the clinic behavior of primary angular closure can range from suspected disease to advanced stages of glaucomatous damage, which can lead to blindness. Objective: to describe the epidemiology of primary angular closure in Pinar del Río patients by identifying the socio-demographic and ocular variables that determine its clinical type. Methods: a cross-sectional descriptive study was performed in Pinar del Río from 2013 to 2017. The target group consisted of 293 new cases with a diagnosis of primary angular closure in any of its clinical types. The statistical analysis was performed with the SPSS program. Results: mean age was 57.69 ± 7.35 years; women predominated (89.5 %) and 77.2 % evidenced a stressful situation. The average values of axial length and anterior chamber width were 21.89 ± 0.49 mm and 2.65 ± 0.27 mm. 87.03 % of the eyes showed a narrow angle. The mean basal intraocular pressure was 23.86 ± 5.81. When analyzing the socio-demographic and ocular variables in relation to the clinical type, it was found that the factors that determine its onset were: age (p<0.001), angular amplitude (p<0.001), peripheral anterior synechia (p<0.001) and intraocular pressure (p<0.001). Linear regression analysis confirmed these results. Conclusion: primary angular closure is common in middle-aged women under stress; with small eyes, camerular angle and narrow anterior chamber. Age, angular amplitude, peripheral anterior synechiae, and basal intraocular pressure determine the clinical type of the disease.

Bacterial cellulose as a potential bioleather substitute for the footwear industry.

Shoe patterns and a sole made of BC, baby shoe made of BC and, on the right BC material dyed in red. (prototype made by www.patent-shoes.com).

[Cognitive implications in the psychophysical estimation of taste]. / Implicaciones cognitivas en la estimación psicofísica del sabor.

INTRODUCTION: in sensory perception of taste, it is difficult to learn the concepts of absolute threshold (AT), the psychophysical methods to estimate it and the influence exerted by prior knowledge on their perception and recognition, because they have little awareness. OBJECTIVE: to assess absolute thresholds of four basic flavors in a sample of healthy young people, in conditions of food restriction/without restriction, and to analyze the influence of prior knowledge of astringency in its detection-identification. METHODS: one hundred and fourteen participants with an average of 20.03 years old (SD = 5.45), 75.4% of them women, were put in direct contact with chemical substances of basic flavors and astringency through a theoretical-practical tasting. An inverted treatment design was applied with experimental conditions on food restriction and knowledge of astringency. RESULTS: all identified their AT for basic flavors. The group with restriction was significantly more sensitive (Tb = -3.305, p = 0.001) to sweet (AU = 2 g/l) than the group without restriction (AU = 5 g/l). The detection-identification of astringency was significantly higher (t = -13.323, p = 0.000) with previous information of the name (79.31%) than without this information (19.64%), confusing or describing it as bitter taste (80.36). CONCLUSION: taste learning is facilitated with the performance of psychophysical measurements of chemical senses, together with training of certain theoretical concepts about taste perception and flavor denomination, because it facilitates the cognitive process for detection-identification. In addition, the process for the previous internal homeostasis of participants must be considered as their AU results vary according to the hunger/satiety conditions prior to the psychophysical estimation.

INTRODUCCIÓN: en la percepción sensorial del gusto, los conceptos de umbral absoluto (UA), los métodos psicofísicos para estimarlo y la influencia que ejerce el conocimiento previo sobre su percepción y reconocimiento son de difícil aprendizaje por su escaso estado de conciencia. OBJETIVO: evaluar los umbrales de detección de los cuatros sabores básicos en una muestra de jóvenes sanos, en condiciones de restricción de alimentos/no-restricción, y analizar la influencia del conocimiento previo de la astringencia para su detección-identificación. MÉTODO: se puso en contacto directo con las sustancias químicas de los sabores y de astringencia a 114 participantes con media de 20,03 años (DT = 5.45), el 75,4% mujeres, mediante una degustación teórica-práctica. Se aplicó un diseño de tratamiento invertido con las condiciones experimentales sobre restricción de alimentos y conocimiento de astringencia. RESULTADOS: todos identificaron sus UA para los cuatro sabores básicos. El grupo con restricción fue significativamente más sensible (Tb = -3,305; p = 0,001) al dulce (UA = 2 g/l) que el grupo sin restricción (UA = 5 g/l). La detección-identificación de la astringencia resultó significativamente mayor (t = -13,323; p = 0,000) con información previa del nombre (79,31%) que sin esta información (19,64%), confundiéndola, o describiéndola, como sabor amargo (80,36%). CONCLUSIONES: el aprendizaje del sabor se favorece cuando se realiza la medición psicofísica de los sentidos químicos junto a la formación de determinados conceptos teóricos sobre la percepción gustativa y denominación de sabores, al facilitar su proceso cognitivo posterior detección- identificación. También debe atenderse al proceso de homeostasis interna previa de los participantes por la variabilidad en los resultados de sus UA según las condiciones de hambre/saciedad anteriores a su estimación psicofísica.

Implicaciones cognitivas en la estimación psicofísica del sabor / Cognitive implications in the psychophysical estimation of taste

Introducción: en la percepción sensorial del gusto, los conceptos de umbral absoluto (UA), los métodos psicofísicos para estimarlo y la influencia que ejerce el conocimiento previo sobre su percepción y reconocimiento son de difícil aprendizaje por su escaso estado de conciencia. Objetivo: evaluar los umbrales de detección de los cuatros sabores básicos en una muestra de jóvenes sanos, en condiciones de restricción de alimentos/no-restricción, y analizar la influencia del conocimiento previo de la astringencia para su detección-identificación. Método: se puso en contacto directo con las sustancias químicas de los sabores y de astringencia a 114 participantes con media de 20,03 años (DT = 5.45), el 75,4% mujeres, mediante una degustación teórica-práctica. Se aplicó un diseño de tratamiento invertido con las condiciones experimentales sobre restricción de alimentos y conocimiento de astringencia. Resultados: todos identificaron sus UA para los cuatro sabores básicos. El grupo con restricción fue significativamente más sensible (Tb = -3,305; p = 0,001) al dulce (UA = 2 g/l) que el grupo sin restricción (UA = 5 g/l). La detección-identificación de la astringencia resultó significativamente mayor (t = -13,323; p = 0,000) con información previa del nombre (79,31%) que sin esta información (19,64%), confundiéndola, o describiéndola, como sabor amargo (80,36%). Conclusiones: el aprendizaje del sabor se favorece cuando se realiza la medición psicofísica de los sentidos químicos junto a la formación de determinados conceptos teóricos sobre la percepción gustativa y denominación de sabores, al facilitar su proceso cognitivo posterior detección-identificación. También debe atenderse al proceso de homeostasis interna previa de los participantes por la variabilidad en los resultados de sus UA según las condiciones de hambre/saciedad anteriores a su estimación psicofísica

Introduction: in sensory perception of taste, it is difficult to learn the concepts of absolute threshold (AT), the psychophysical methods to estimate it and the influence exerted by prior knowledge on their perception and recognition, because they have little awareness. Objective: to assess absolute thresholds of four basic flavors in a sample of healthy young people, in conditions of food restriction/without restriction, and to analyze the influence of prior knowledge of astringency in its detection-identification. Methods: one hundred and fourteen participants with an average of 20.03 years old (SD = 5.45), 75.4% of them women, were put in direct contact with chemical substances of basic flavors and astringency through a theoretical-practical tasting. An inverted treatment design was applied with experimental conditions on food restriction and knowledge of astringency. Results: all identified their AT for basic flavors. The group with restriction was significantly more sensitive (Tb = -3.305, p = 0.001) to sweet (AU = 2 g/l) than the group without restriction (AU = 5 g/l). The detection-identification of astringency was significantly higher (t = -13.323, p = 0.000) with previous information of the name (79.31%) than without this information (19.64%), confusing or describing it as bitter taste (80.36). Conclusion: taste learning is facilitated with the performance of psychophysical measurements of chemical senses, together with training of certain theoretical concepts about taste perception and flavor denomination, because it facilitates the cognitive process for detection-identification. In addition, the process for the previous internal homeostasis of participants must be considered as their AU results vary according to the hunger/satiety conditions prior to the psychophysical estimation

Correction: New localization and function of calpain-2 in nucleoli of colorectal cancer cells in ribosomal biogenesis: effect of KRAS status.

[This corrects the article DOI: 10.18632/oncotarget.23888.].

New localization and function of calpain-2 in nucleoli of colorectal cancer cells in ribosomal biogenesis: effect of KRAS status.

Calpain-2 belongs to a family of pleiotropic Cys-proteases with modulatory rather than degradative functions. Calpain (CAPN) overexpression has been controversially correlated with poor prognosis in several cancer types, including colorectal carcinoma (CRC). However, the mechanisms of substrate-recognition, calpain-2 regulation/deregulation and specific functions in CRC remain elusive. Herein, calpain subcellular distribution was studied as a key event for substrate-recognition and consequently, for calpain-mediated function. We describe a new localization for calpain-2 in the nucleoli of CRC cells. Calpain-2 nucleolar distribution resulted dependent on its enzymatic activity and on the mutational status of KRAS. In KRASWT/- cells serum-starvation induced CAPN2 expression, nucleolar accumulation and increased binding to the rDNA-core promoter and intergenic spacer (IGS), concomitant with a reduction in pre-rRNA levels. Depletion of calpain-2 by specific siRNA prevented pre-rRNA down-regulation after serum removal. Conversely, ribosomal biogenesis proceeded in the absence of serum in unresponsive KRASG13D/- cells whose CAPN2 expression, nucleolar localization and rDNA-occupancy remained unchanged during the time-course of serum starvation. We propose here that nucleolar calpain-2 might be a KRAS-dependent sensor to repress ribosomal biogenesis in growth limiting conditions. Under constitutive activation of the pathway commonly found in CRC, calpain-2 is deregulated and tumor cells become insensitive to the extracellular microenvironment.

Utilidad de la gammagrafía con leucocitos marcados y SPECT/TC en la infección de injertos vasculares / Usefulness of scintigraphy with labelled leucocytes and SPECT/CT in vascular graft infection

La infección de injertos vasculares es una complicación poco frecuente en este tipo de procedimientos. Sin embargo, cuando se presentan suelen tener una alta morbilidad e incluso una alta mortalidad. Su adecuada identificación y localización es crucial para el pertinente y temprano manejo, ya sea médico o quirúrgico, por lo que el conocimiento de las herramientas adecuadas es primordial. Los estudios de medicina nuclear tienen un importante papel en este sentido, ya sea mediante gammagrafía con leucocitos marcados o con 18F-FDG. La elección dependerá de la experiencia con ambas técnicas de los diferentes grupos, del conocimiento de las mismas y de su disponibilidad, entre otros factores. Presentamos 2 casos en los que la gammagrafía con 99mTc-HMPAO-leucocitos autógenos marcados-SPECT/TC fue de gran utilidad en el diagnóstico y localización del compromiso infeccioso sospechado y, por lo tanto, en el adecuado y guiado manejo posterior. Ambos casos tuvieron confirmación clínica y microbiológica (AU)

Vascular graft infections are a rare complication in this type of procedure. However, when they do occur, they usually have high morbidity, and even a high mortality. Proper identification and location is crucial for the appropriate and early management, whether medical or surgical, thus knowledge of the right tools is paramount. Nuclear medicine studies play an important role in this regard, either by using labelled white blood cells scintigraphy or 18F-FDG. The choice, among other factors, will depend on the experience with both techniques by the different groups, their knowledge of them, as well as their availability. Two cases are presented in which 99mTc-HMPAO-white blood cells SPECT/CT scintigraphy was very useful in the diagnosis and location of the suspected infectious compromise, which led to the subsequent appropriate and guided management. Both cases were confirmed clinically and microbiologically (AU)

Usefulness of scintigraphy with labelled leucocytes and SPECT/CT in vascular graft infection. / Utilidad de la gammagrafía con leucocitos marcados y SPECT/TC en la infección de injertos vasculares.

Vascular graft infections are a rare complication in this type of procedure. However, when they do occur, they usually have high morbidity, and even a high mortality. Proper identification and location is crucial for the appropriate and early management, whether medical or surgical, thus knowledge of the right tools is paramount. Nuclear medicine studies play an important role in this regard, either by using labelled white blood cells scintigraphy or 18F-FDG. The choice, among other factors, will depend on the experience with both techniques by the different groups, their knowledge of them, as well as their availability. Two cases are presented in which 99mTc-HMPAO-white blood cells SPECT/CT scintigraphy was very useful in the diagnosis and location of the suspected infectious compromise, which led to the subsequent appropriate and guided management. Both cases were confirmed clinically and microbiologically.