RESUMO
OBJECTIVES: To evaluate the influence of the delay in urinary NMP22 preanalytical processing. MATERIAL AND METHODS: Twenty-eight voided urine samples were taken: bladder cancer (14), urine tract infections (4), lithiasis (4), healthy volunteers (1), and with other no malignant bladder diseases (5). All samples, were maintained at environment temperature, and were processed according to the stabilization of parts of urine collected at 0, 30, 90 and 150 minutes. Samples were stored at 4 degrees C until its determination. NMP22 was determined with the IMMULITE One analyzer. RESULTS: There were no significant differences for NMP22 levels between each different point of time studied. CONCLUSIONS: Delay up to 2 hours and a half when we add stabilization solution to urine samples no affects NMP22 results. That thing, might provide more confidence and flexibility on quantitative immunoassays that required urine stabilization.
Assuntos
Biomarcadores Tumorais/urina , Proteínas Nucleares/urina , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fatores de TempoRESUMO
Objetivo: Evaluar la influencia del retraso en el procesamiento preanalítico de orinas en las que se realiza la determinación del marcador tumoral NMP22. Material y métodos: Se recogieron 28 muestras de orina: tumores vesicales (14), infecciones urinarias (4), litiasis (4), voluntarios sanos (1), y otras patologías vesicales benignas (5). De cada muestra, mantenida a temperatura ambiente, se fueron estabilizando alícuotas con el conservante suministrado por el fabricante a los 0, 30, 90 y 150 minutos, guardándose a 4ºC hasta su procesamiento. El análisis del NMP22 se realizó en autoanalizador IMMULITE One. Resultados: No se apreciaron diferencias significativas en los niveles del NMP22 entre los diferentes puntos de demora estudiados. Conclusiones: La demora de hasta dos horas y media en la adición de la solución conservante a la orina para determinación de NMP22 no afecta significativamente a los resultados obtenidos. Este hecho permite una mayor confianza y flexibilidad en los inmunoensayos cuantitativos que requieren estabilización de la muestra
Objectives: To evaluate the influence of the delay in urinary NMP22 preanalytical processing. Material and methods: Twenty-eight voided urine samples were taken: bladder cancer (14), urine tract infections (4), lithiasis (4), healthy volunteers (1), and with other no malignant bladder diseases (5). All samples, were maintained at environment temperature, and were processed according to the stabilization of parts of urine collected at 0, 30, 90 and 150 minutes. Samples were stored at 4 ºC until its determination. NMP22 was determined with the IMMULITE One analyzer. Results: There were no significant differences for NMP22 levels between each different point of time studied. Conclusions: Delay up to 2 hours and a half when we add stabilization solution to urine samples no affects NMP22 results. That thing, might provide more confidence and flexibility on quantitative immunoassays that required urine stabilization
Assuntos
Humanos , Métodos de Análise Laboratorial e de Campo , Química Analítica , 24968 , Fatores de TempoRESUMO
We present 3 new cases of urethral parameatal cysts in paediatric age. Literature is reviewed and histological data and ambulatory management are discussed.
Assuntos
Cistos/cirurgia , Doenças Uretrais/cirurgia , Criança , Pré-Escolar , Humanos , MasculinoRESUMO
Presentamos 3 nuevos casos de quistes uretrales parameatales en edad pediátrica. Se revisa la literatura y comentamos los hallazgos histológicos y el manejo ambulatorio de estos pacientes
We present 3 new cases of urethral parameatal cysts in paediatric age. Literature is reviewed and histological data and ambulatory management are discussed
