Patterns in utilization of carbon sources in soil microbial communities contaminated with mine solid wastes from San Luis Potosi, Mexico.

In San Luis Potosí, Mexico, the exploitation of minerals has historically been carried out as an activity that has left in its path environmental liabilities, with high concentrations of heavy metals. These metals have undergone weathering by rain and wind and have moved closer to inhabited locations as is the case of Cerro de San Pedro (CSP) and Villa de la Paz (VDP). The objective of this study is to show the biological alteration of soils due to the presence of heavy metals and metalloids like Cadmium (Cd), Copper (Cu), Lead (Pb) and Arsenic (As) and to find the relationship between contamination and risk indexes. Soil samples were obtained from sites with historical records of mining activity and their surroundings. Several analyses were performed, such as pH levels, organic matter, electrical conductivity, clays, heavy metals and As. Moreover, Community Level Physiological profiling (CLPP) were conducted. The obtained evidence showed high levels of contamination by As and heavy metals in both sites (CSP: 6485.1 mg/Kg of Pb and pH of 4.4; VDP: 7188.2 mg/Kg of As and pH of 7.8). According to the Metal Pollution Index (MPI), 607.0 in CSP and 1050.5 in VDP, presented a high environmental risk, apart from, risk to human health (SQGQI) 35.8 in CSP and 131.5 in VDP. At the same time, CLPPs showed that microbiological communities were selective in taking up substrate groups, in the following order: Carbohydrates > Polymers > Carboxylic acids > Amino acids > Amines/Amides. However, a positive correlation in CSP was only found between both indexes and Amines/Amides (r = 0.46, p < 0.05), and in VDP the D-Galactonic acid-γ-Lactone with the MPI (r = 0.49, p < 0.05), and with the SQGQI (r = 0.45, p < 0.05). Although this behavior was not homogeneous, it was possible to find negative correlations between both indexes and the AWCD with other substrates, influenced by the physicochemical characteristics presented in each studied site. Consequently, according to our findings, a combined effect between the physicochemical characteristics, As, and heavy metals took place, on the metabolic activity, causing alterations to soil functions.

Integrated probabilistic risk assessment in sites contaminated with arsenic and lead by long-term mining liabilities in San Luis Potosi, Mexico.

Mining environmental liabilities (MEL) in San Luis Potosi are the result of more than 450 years of mining activity, which has contaminated the soil mainly with arsenic (As) and lead (Pb) in several areas. Risk assessments are used to estimate the possibility of the occurrence of adverse effects on human health or on ecological receptors; and the most accessible way of performing them is through probabilistic estimates such as the Latin Hypercube Sampling (LHS) model. Therefore, the aim of this study was to carry out an Integrated Probabilistic Environmental Risk Assessment (IPERA) for the estimation of health risks in infants and rodents. The mean concentrations of As and Pb in soil were significantly higher (p<0.05) in all contaminated sites than in their respective reference sites. Villa de la Paz was the site with the highest mean concentration of As (1374 mg/kg), while Charcas was the one with the highest level of Pb (12,929 mg/kg). The Hazard Quotient (HQ) was calculated and Villa de la Paz had the highest values of As in both rodents (11.994) and children (39.32), and Charcas showed the highest values of Pb in both (24.971 and 31.668 for rodents and children respectively). The cumulative hazard Index (HI) reveals there is a very significant health risk due to As and Pb exposure for both rodents and children in contaminated areas of these mining communities.