RESUMO
High Mobility Group B (HMGB) proteins are involved in cancer progression and in cellular responses to platinum compounds used in the chemotherapy of prostate and ovary cancer. Here we use affinity purification coupled to mass spectrometry (MS) and yeast two-hybrid (Y2H) screening to carry out an exhaustive study of HMGB1 and HMGB2 protein interactions in the context of prostate and ovary epithelia. We present a proteomic study of HMGB1 partners based on immunoprecipitation of HMGB1 from a non-cancerous prostate epithelial cell line. In addition, HMGB1 and HMGB2 were used as baits in yeast two-hybrid screening of libraries from prostate and ovary epithelial cell lines as well as from healthy ovary tissue. HMGB1 interacts with many nuclear proteins that control gene expression, but also with proteins that form part of the cytoskeleton, cell-adhesion structures and others involved in intracellular protein translocation, cellular migration, secretion, apoptosis and cell survival. HMGB2 interacts with proteins involved in apoptosis, cell motility and cellular proliferation. High confidence interactors, based on repeated identification in different cell types or in both MS and Y2H approaches, are discussed in relation to cancer. This study represents a useful resource for detailed investigation of the role of HMGB1 in cancer of epithelial origins, as well as potential alternative avenues of therapeutic intervention.
RESUMO
The eukaryotic Ssu72 factor is involved in several RNA biogenesis processes. It has phosphatase activity on the carboxy-terminal domain (CTD) of the major subunit of RNA polymerase II. The Kluyveromyces lactis Ssu72 (KlSsu72) shows in vitro phosphatase activity for the pNPP substrate, and this activity is inhibited by ortho-vanadate. The expression of KlSsu72 in Saccharomyces cerevisiae shows defective CTD serine5-P phosphatase activity and reveals the importance of Ssu72 for the normal CTD serine5-P levels at two growth states. The divergence is emphasised by the remarkable changes in RNA14 alternative 3'-end RNA processing, which are independent of the CTD serine5-P levels.
Assuntos
Kluyveromyces/metabolismo , Fosfoproteínas Fosfatases/química , Proteínas de Saccharomyces cerevisiae/química , Saccharomyces cerevisiae/metabolismo , Fatores de Poliadenilação e Clivagem de mRNA/química , Sequência de Aminoácidos , Domínio Catalítico , Sequência Conservada , Dados de Sequência Molecular , Fosforilação , Ligação Proteica , RNA/análise , RNA/metabolismo , Homologia de Sequência de Aminoácidos , Transcrição Gênica , Vanadatos/farmacologiaRESUMO
No disponible
Assuntos
Humanos , Infecções por Helicobacter/terapia , Bombas de Próton/antagonistas & inibidores , Úlcera Péptica Perfurada/terapia , Helicobacter pylori/patogenicidade , Técnicas de SuturaRESUMO
El mesotelioma quístico peritoneal es una lesión caracterizada por la formación de múltiples quistes de tamaño variable que adoptan la forma de masas abdominales preferentemente pelvianas. Presentamos el caso de una mujer de 30 años con dolor abdominal y masa palpable cuya clínica sugería un cuadro de endometriosis. En la intervención quirúrgica se encontró una masa multiquística dependiente del epiplón mayor, por lo que se procedió a su extirpación. El diagnóstico anatomopatológico de la pieza fue de mesotelioma multiquístico peritoneal benigno, una enfermedad poco frecuente, en la que el problema más importante que se plantea es la alta tasa de recidiva locorregional (AU)
