The effect of long-term exposure to combinations of growth promoters in Long Evans rats: part 1: Endocrine adrenal function.

UNLABELLED: The aim of the study was to investigate whether the chronic administration (45 days) of clenbuterol (CB) at a growth promoting dose (1 mg kg(-1) bw) and/or dexamethasone (DEX: 0.1 mg kg(-1) bw) may cause the disruption of rat endocrine adrenal function. Blood samples were taken weekly during the whole experiment (S0-S7), and at different days of withdrawal (W0, W5, W10, W15 and W20). Hormone profiles were determined by RIA (ACTH) or EIA (corticosterone and catecholamines). ACTH showed significantly elevated concentrations from S1 until W5 (p<0.05) with CB administration. It began to decrease the day of DEX and CB-DEX administration. DEX showed significantly lowered ACTH concentrations from the day of drug injection (p<0.05). Corticosterone showed significantly elevated levels until W10 (p<0.01) with CB and CB+DEX. DEX showed lowered levels of corticosterone during the whole withdrawal period. Epinephrine presented significantly elevated plasma levels until W5 with CB and CB+DEX. With DEX, epinephrine was also elevated from W5 to W15 (p<0.05). Norepinephrine also presented significantly elevated plasma levels until S7 with CB and CB+DEX (p<0.001). With DEX no differences were found. CONCLUSION: Long-term administration of CB and/or DEX causes an endocrine adrenal disruption with changes in ACTH, glucocorticoid and catecholamine secretion.

The effect of long-term exposure to combinations of growth promoters in Long Evans rats: part 2. Adrenal morphology (histopathology and immunochemical studies).

The aim of the study was to investigate the effects of long-term exposure (45 days) to growth promoters: clenbuterol (CB: 1 mg kg(-1) bw) and/or dexamethasone (DEX: 0.1 mg kg(-1) bw), in adrenal gland morphology, and the possibility of recovery after the withdrawal of drug treatment. Animals were sacrificed at different days of withdrawal (W0, W5, W10, W15 and W20), and adrenal glands processed for histopathology and immunohistochemistry. Adrenals of CB treatment showed typical features of long-term administration of beta-agonists at W0 such as capillary dilatation in the fasciculata-reticularis zone, and this feature was also presented at W20. Adrenals of CB+DEX treatments showed the same results of CB treatment at days W0 and W20. However, DEX treatment presented the typical results of the exposure to corticoids with the atrophy of adrenal cortex. Immunohistochemistry of adrenal cortex steroidogenic enzymes (P450: scc, 3beta-HSD, aromatase) denoted that neither positive staining nor localization was affected by treatments. Aromatase enzyme was immunolocalized in adrenal medulla cells in controls as well as in treated groups. The immunolocalization of glucocorticoid receptors showed an increase in CB (+++) and CB+DEX (++) treatments compared to the control group (0) and DEX treatment (0). Histopathological and immunohistochemical results are closely related to those found for adrenal endocrine function. We can conclude that chronic administration of growth promoters influence adrenal morphology and glucocorticoid receptor expression.

Use of dogs as indicators of metal exposure in rural and urban habitats in NW Spain.

Many different species have been used in environmental biomonitoring studies in diverse habitats including forest, farmland, and urban and sub-urban areas. However, there is little information on domestic animals living in rural or urban habitats and exposed to the same pollutants as the human population. In this connection, pets could prove to be good indicators of human metal exposure since they closely share the same environment as their owners, and are therefore exposed, at least in part, to the same pollutants. The present study investigated toxic metal exposure in dogs in NW Spain and compared metal exposures between dogs from rural and urban habitats, considering the influence of diet, sex and age. Samples of liver and kidney from 57 male and female dogs, aged between 6 months and 18 years, were collected after euthanasia at veterinary clinics. Samples were acid-digested and metal concentrations determined by ICP-MS. Geometric mean concentrations of metals in the liver and kidney (microg/kg wet weight) were 12.6 and 15.9 for arsenic, 58.0 and 175 for cadmium, 32.7 and 53.4 for mercury, and 57.7 and 23.1 respectively. Hepatic lead concentrations were significantly higher (p<0.05) in dogs fed commercial diets than dogs fed home-made feed (32%) or a mixture of commercial and home-made feeds (95%). Mercury concentrations in the kidney were significantly higher (3-fold, p<0.05) in dogs from urban areas than in dogs from rural areas. Cadmium levels in kidney were significantly higher (p<0.05) in females (67%) and increased with age (p<0.001). Although no human samples were obtained in this study and no direct correlations between dogs and human metal exposure have been conducted, given our results pets could be suggested as surrogate indicators of human metal exposure.

Long-term follow-up of blood lead levels and haematological and biochemical parameters in heifers that survived an accidental lead poisoning episode.

Four of 10 heifers that survived an episode of lead poisoning caused by an old broken battery in the pasture were periodically monitored from days 14 to 205 after the episode, with the aim of (i) determining the time-course of lead clearance from blood; and (ii) assessing whether the exposed cattle showed changes in haematological parameters or serum markers of hepatic and renal function (e.g. serum creatinine level) that might be useful as indicators of subclinical toxicity. On the first sampling day, lead levels in blood varied between 0.478 and 0.758 mg/l. The half-life of lead in blood was rather variable, ranging from 68 to 266 days. Despite the high blood lead levels, no significant changes were detected in haematological parameters or hepatic/renal markers, all of which remained within normal ranges. In conclusion, these cattle that survived an episode of lead poisoning may constitute a risk for human consumers, but do not appear to have suffered severe health consequences themselves.

Modifications of the form and amplitude of the electrocardiographic QRS complex during growth in the Spanish-bred Horse.

Configuration and amplitude values of the QRS complex of the electrocardiogram were studied with standard Einthoven leads in 173 healthy Spanish-bred (Andalusian) horses, aged between 1 month and 17 years. Animals which were 1 month old had a predominantly negative QRS complex, whereas a predominantly positive complex direction was found in the rest of the animals. Statistically significant variations were found for the Q-wave and QRS main vector between the different age groups, with highest values for the Q-wave and most negative values for the direction of the QRS main vector in animals up to 2 months of age. The effect of age on the form and amplitude values of the QRS complex could be related to the changes in size and position of the heart in the thorax during growth. This study provides standard electrocardiographic values for the Andalusian horse at different ages.

Urinary hydroxyproline excretion in male and female chickens.

Hydroxyproline was measured in 24-hr urine samples at 60 and 120 days of age in male and female broiler chickens that were prepared by the "anus praeternaturalis" technique. Twenty-four-hour urine output did not differ between females and males (32.82 +/- 1.02 ml and 33.98 +/- .54 ml, average +/- SD, respectively), whereas 24-hr urinary hydroxyproline excretion was greater in females than in males after 60 days of age. Urinary hydroxyproline excretion tended to decrease with age in males and increase with age in females. Because most of the hydroxyproline is probably derived from bone collagen, the results suggest that there are differences between mineral exchange processes in female and male chickens.