Implementation and analysis of Babesia immunoassay testing.

Lifelong withdrawal from the donor population of those who have been diagnosed with babesiosis must be used for transmission prevention. AIM: The aim of the study was a detection of Babesia antibodies level with the usage of experimental Babesia divergens whole-cell slide antigen and commercial B. microti immunofluorescence assay substrate slide (Fuller Laboratories, USA). MATERIALS AND METHODS: Experimental B. divergens whole-cell slide antigen in addition to commercial B. microti IFA substrate slide was used to create a diagnostic kit for serum Babesia antibodies level detecting, as well as for a babesiosis serodiagnosis clinical trial of different origins blood samples (patients with Lyme disease, rheumatoid arthritis and toxoplasmosis; human blood donors; cattle). RESULTS: Antibodies to B. divergens (5.4%) and B. microti (2.3%) were detected with higher (p <0.05) frequency at Lyme disease patients (16.7%) than at blood donors (1.7%). Diagnostically significant IgG titres (= 1:128) were found in 13.3% of blood samples from Lyme disease patients and 1.7% from blood donors. Specific IgM were also found in 13.3% blood samples from Lyme disease patients. Among blood samples from Lyme disease patients, in which diagnostically significant titres of Babesia antibodies were detected (16.7%), 60% of them were represented by IgG and IgM (rA= 0.63), and in 40% only one of them reached diagnostically significant titre. Conclusions. Advantages of babesiosis IFA diagnostics. CONCLUSIONS: Advantages of babesiosis IFA diagnostics are combined with its significant disadvantages (principle of evaluation, low sensitivity in the initial period of the disease, probability of false positives, absence of validated test systems and research protocols for B. divergens and B. divergens-like species).

A METHOD OF COMPLEX EVALUATION OF MORPHOLOGICAL STRUCTURE OF OSTIOMEATAL COMPLEX COMPONENTS, LOWER WALL OF MAXILLARY AND FRONTAL SINUSES.

OBJECTIVE: The aim: Was to evaluate the anatomical variability of the frontal and maxillary sinuses, ostiomeatal complex components (OMC) and to identify factors that contribute to complications of inflammatory processes and development of odontogenic maxillary sinusitis. PATIENTS AND METHODS: Materials and methods: The study involved assessment of 100 spiral computed tomograms (SCT) of human patients without pathological processes in the PNS area. The basic parameters of the anatomical structure of the ostiomeatal complex (the area of the hooked process and the middle nasal conch, their transverse dimensions, the density and dimensions of the natural connection), the thickness and the density of the lower wall of the maxillary and frontal sinuses were determined. These parameters were investigated by the method of uncertainty calculation. RESULTS: Results: The findings showed that the bone density of the maxillary sinus on the left was 57.713 ± 440.356 Hu (minimum), 1101.507 ± 613.4882 Hu (maximum); 96.2752 ± 395.0 and 1028.691 ± 620.4051 on the right, respectively, the density of the inferior frontal sinus wall on the right was 5.5179 ± 276.43 and 831.1607 ± 732.274, on the left 12.069 ± 310.56 and 898.293 ± 748, respectively. In the same way, the probable OMC structure parameters, in the range ± U at the confidence level p = 0.95, were calculated. CONCLUSION: Conclusions: Thus, some variants of the anatomical structure of the ostiomeatal complex can be a prerequisite for hypoventilation of PNS and, as a consequence, lead to inflammatory processes in them. Features of the same structure of the walls of the PNS are a prerequisite for the propagation of the inflammatory process in the surrounding tissues and the development of complications.

Anaplasmosis: experimental immunodeficient state model.

OBJECTIVE: Introduction: The recently described anaplasmosis infection is widespread but concerns to the insufficiently known group of diseases. The aim of our research is the development of uniform biological model for reproducing of artificial immunodeficient state by experimental anaplasmosis. PATIENTS AND METHODS: Materials and methods: Algorithm of experimental anaplasmosis reproducing, consisted of such consecutive stages: 1) artificial forming of the immunodeficient state at nonlinear white mise (Mus musculus L.); 2) preparation of the tested biological material samples; 3) inoculation by prepared samples of the laboratory animals with the artificially formed immunodeficient state; 4) sampling from the dead or slaughtered (by the method of chloroformed anesthesia) experimental animals of sectional material (organs and targets tissues); 5) verification of aetiology by express detection of causative agents by the method of PCR in the selected samples of sectional material. RESULTS: Results: Biological model of experimental anaplasmosis have been created suitable for realization of both diagnostic and epidemiological, epizootic, ecobiological and other researches of different origin biological material samples, including samples of solid and liquid consistency material. Formed model realised in premature death of experimental animals in 17.4 % cases; resulted in an onset of disease clinical signs without death during the term of supervision in 43.8 % cases; coursed in the absence of the expressed symptoms of infection in 31.3 % cases. CONCLUSION: Conclusions: Developed biological model of experimental anaplasmosis consists in that as laboratory animals with the increased sensitiveness to the infection and accumulation of causative agent are used white nonlinear mice with the artificially formed immunodeficient state.