[Peculiarities of diagnosis and treatment of patients with hyposialosis].

33 patients with complaints of hyposalivation (21 in a 33 with syaloadenopatya and 12 in a 33 with syalosis out of the acute stage of disease) were examined. Complex examination of the saliva glands, analysis of the haemokynes in the various mediums of organism and thyoles compounds in the blood plasma was carried out. Tendency to increase in contents of haemokynes in the mixed saliva and in the tissue samples (from low in the syaloadenopatya to expressed in the syalosis) was revealed. Reduction of thyosulfide index in the blood plasma of these patients was determined. Use of antioxidant remedy in complex treatment of syaloadenopatya and syalosis was substanciated in this paper.

[Experimentally determined safety and immunological activity of vaccine based on antigens isolated from Pseudomonas aeruginosa in medium K-4].

The safety and immunological activity of P. aeruginosa vaccine were experimentally evaluated. The vaccine was prepared on the basis of the antigens of P. aeruginosa extracellular slime which was accumulated in medium K-4, obtained with the use of original technology. The immunization of animals with P. aeruginosa vaccine induced the synthesis of antibodies. The introduction of the vaccine in 2 or 3 injections resulted in a high level of antibody formation, differing with the use of various strains. Hyperimmune sera, obtained by the multiple immunization of rabbits with P. aeruginosa vaccine, ensured high protection of mice from P. aeruginosa infection. The vaccine proved to be safe when evaluated in experiments of acute and chronic toxicity, made on laboratory animals.

[Pseudomonas aeruginosa vaccine on the basis of antigens isolated from the supernatant of culture media K-4]. / Sinegnoinaia vaktsina na osnove antigenov, vydelennykh iz supernatanta sredy kul'tivirovaniia K-4.

The possibility of using experimental culture medium K-4 prepared on the basis of casein hydrolysate peptides with the isoelectric point 4.1 for obtaining antigens from P. aeruginosa strains was evaluated. Two antigenic fractions were isolated from the culture fluid containing extracellular slime. The study of the toxicity of the antigenic preparations revealed that one of these fractions had low toxicity for mice (the second antigenic fraction was highly toxic). The former P. aeruginosa antigenic fraction was used for obtaining pyocyanic vaccine. One vaccination dose of this vaccine contained 0.2 mg of the antigen adsorbed on aluminum hydroxide. Pyocyanic vaccine ensured the active protection of mice challenged with P. aeruginosa homologous and heterologous strains.

[Study on the effect of a biostimulant on the growth and toxigenic function of Clostridium tetani strain Copenhagen-471]. / Izuchenie vliianiia biostimuliatora na rost i toksinoobrazuiushchuiu funktsiiu Clostridium tetani "Kopenhagen-471."

Bakstim, a new biostimulating preparation obtained from the organs of the immune system of animals, was developed. The impact of Bakstim on the growth and toxigenic function of C. tetani production strain Copenhagen-471 was evaluated. The addition of the preparation to Gluzman commercial medium for obtaining tetanus toxoid led to an increase in the yield of bacterial biomass from 1.9 to 4-fold and an increase in the toxoid production from 2 to 2.8-fold. The optimum concentration of this biostimulant ensuring the maximum yield of tetanus toxin from the production culture was determined (1,000 mg/l). Bakstim will supposedly be used as additive to nutrient media for the production of tetanus toxoid.

[Effect of gravitational tidal changes on the periodicity of T-lymphocyte receptor expression in vitro]. / Vliianie prilivnykh izmenenii sily tiazhesti na periodichnost' ékspressii retseptorov T-limfotsitov in vitro.

The hourly registration of the expression of the human s T-lymphocytes subpopulations receptors in vitro during 6 hours demonstrated the existence of the periodicity from 2 to 4 hours. There is high degree of T-lymphocytes expressions coincidence from different individuals depending of the date. For most of daily average individual chronograms, there were described back linear and valid correlations with a character of heterogeneity of the gravity force changes. The results are in according to suggestion about the time field geometric effect to structural-functional cells periodicity.

[Biohemosorption on xenospleen as a method for detoxication in burns]. / Biogemosorbtsiia na ksenoselezenke kak metod detoksikatsii pri ozhogovoi bolezni.

Extracorporal inclusion of the porcine spleen was used in 180 patients with burns of the III A-B-IV degrees with the square area of burns from 25% to 95% of the body surface. The age of the patients was from 1 to 60 years. Critical trauma was diagnosed in 48 patients, supercritical--in 132 patients. The total amount of procedures was 310. Positive dynamics in the course of the burn disease was observed after biohemosorption; decrease of the body temperature, of intoxication delirium symptoms, of respiratory insufficiency. The state of the burn wounds was improved. It was stated that the liver function became better, indicators of nonspecific factors of defense, cellular and humoral links of immunity were increased, a tendency to normalization of lipid metabolism was noted. Positive results were obtained in 43 of 48 with critical trauma. Recovery took place in 52 of 132 patients with supercritical traumas.

[A complex method of detection of Helicobacter pylori in patients with duodenal ulcer]. / Kompleksnyi metod vyiavleniia Helycobacter pylori u bol'nykh iazvennoi bolezn'iu dvenadtsatiperstnoi kishki.

A method for measurements of blood antigen-binding lymphocytes sensitized to Helicobacter antigens is suggested. Use of this method together with urease test will improve Helicobacter infection detection rate by 22%.

[The development of intestinal dysbacteriosis in sexually mature rats after thymectomy]. / Razvitie disbakterioza kishechnika u polovozrelykh krys posle timéktomii.

Thymectomy in puberal rats resulted in the development of pronounced enteric dysbacteriosis, correlating with the degree of T- and B-lymphocyte deficiency in the lymphoid organs and the blood stream during the first 2 months. Microbiocenosis restores probably due to more intensive functioning of alternative regulating mechanisms. A simple and informative characteristic indicating the degree of dysbacteriosis developing in the intestine is proposed: the index of dysbacteriosis.

[Immune disorders and sensitization to microbial antigens in seronegative spondylitis]. / Immunnye narusheniia i sensibilizatsiia k mikrobnym antigenam pri seronegativnykh spondyloartritakh.

A study was made of immunity in seronegative spondylarthritis. In ankylosing spondylarthritis, reactive arthritides and psoriatic arthritis, the decrease of T cells, T helpers and T suppressors was identified. Patients with ankylosing spondylarthritis and reactive arthritides manifested an increase of the B-lymphocyte count. Patients with ankylosing spondylarthritis were demonstrated to be sensitive to Klebsiella (52%), those with reactive arthritides to intestinal yersinia (44%). The phenotype of antigen-binding cells was examined. Of these, 39% appeared to be T lymphocytes, 29% B lymphocytes. The presence of Fc receptors in an appreciable portion of those cells suggests their functional maturity. The inhibition test demonstrated the participation of HLA-B27 in reception of Klebsiella antigens in ankylosing spondylarthritis.

[Intestinal microbial ecology and its modulation under the influence of immunodepressants]. / Mikrobnaia ékologiia kishechnika i ee izmenenie pod vliianiem immunodepressantov.

Oral administration of immunodepressants such as imuran (purine analog) and batriden (gossypol derivative) for 3 months led to development of dysbacterioses in various sections of the rat gastrointestinal tract. The dysbacterioses differed in their levels and the pattern of the recovery process. As compared to batriden, imuran in a dose of 30 mg/kg body weight administered at the early observation periods (days 7, 14 and 30) induced more marked disorders in the intestine microecology. The imuran-induced dysbacteriosis was characterized by lower quantities of lactobacilli and bifidobacteria in the rat intestine. After the use of batriden the quantities of bifidobacteria, lactobacilli and bacteroides decreased. After the batriden use at the late observation periods (days 60 to 90) the ratio of anaerobes and lactobacilli to aerobes recovered at the background of increased quantities of Candida in all the intestine sections while the ratio of bacteroides recovered in the stomach. When immunity was suppressed by imuran the recovery period was characterized by normalization of the microflora composition in the distal sections and preservation of the contamination symptom in the proximal section which was evident from predominance of aerobes over anaerobes.

[Dynamics of various indicators of cellular immunity in patients with diffuse toxic goiter after drug therapy]. / Dinamika nekotorykh pokazatelei kletochnogo immuniteta u bol'nykh diffuznym toksicheskim zobom pod vliianiem medikamentoznoi terapii.

The purpose of the investigation was to study some indices of the immune system of patients with diffuse toxic goiter (DTG) before multimodality thyrostatic therapy and after achieving clinical remission. A total of 30 DTG patients with an average severity of disease were examined. The immune status was evaluated according to the content of T-lymphocytes, T-suppressors, Fc gamma-lymphocytes and antigen-binding lymphocytes (ABL) responsive to thyroglobulin. Conservative therapy included mercazolyl, beta-adrenergic blockers, sedative and cardiac drugs. A drop of T1T gamma and Fc gamma-lymphocytes was revealed in the period of the manifestation of the clinical signs of disease. The indices of ABL responsive to thyroglobulin significantly exceeded the relative and absolute indices of the control group. Multimodality drug therapy resulted in the deepening of T-lymphocyte deficiency, a significant rise of T gamma 1 and T gamma 2 as compared to the initial levels. A tendency to a decrease in the level of Fc gamma-lumphocytes was noted after the therapy. The relative and absolute values of the ABL indices significantly decreased, however a significant increase in these indices compared to the control ones maintained.