[Increase of pathogenicity of Staphylococcus aureus strains caused by lysogenic conversion by phages of serologic group F]. / Wzrost patogennosci szczepów gronkowca zlocistego spowodowany konwersja lizogenna fagami grupy serologicznej F.

The substantial influence of presence of the double converting prophages of the serological group F on the decrease of intensity of human leukocytes stimulation with lysogenic cells of S. aureus was determined. The end values of chemiluminescence in the six experimental systems containing lysogenic bacteria were only from 15.4 to 37.2% of the value in the control sample. The end values of the intercellular killing of the two lysogenic variants were twofold lower than in controls. Third of the investigated variants was completely resistant to intracellular killing in the applied system, and revealed 27% of growth stimulation.

Evaluation of the antineoplastic activities of the riminophenazine agents clofazimine and b669 in tumor-bearing rats and mice.

The riminophenazine agents clofazimine and its analogue B669 displayed anti-tumor activity at 30 mg/kg/day in benzo[a]pyrene (BP) induced sarcomas of mice as well as dimethylbenz-anthracene (DMBA)-induced rat mammary tumors. No hematological toxicity of these drugs at doses up to 60 mg/kg/day for one month was observed. This is the first study to document in vivo anti-neoplastic activities of clofazimine and B669.

The effect of vitamin E on granulocyte function in patients with recurrent infections.

The influence of vit. E treatment on metabolic and bactericidal granulocyte activity in patients with recurrent infections was studied by chemiluminescence and phagocytosis assays. Vit. E serum level was significantly diminished before therapy. Six weeks supplementation with 200-300 mg/day of vit. E resulted in improvement of clinical status and normalisation of alpha-tocopherol serum level. Bactericidal activity of granulocytes was lower in patients than in the respective controls and it slightly rose after vit. E therapy. Before, therapy, patients granulocytes showed metabolic activity above or within the control range. Vit. E treatment increased, had no effect or decreased granulocyte metabolic activity, but never below the control range.

[Effect of aspirin on FMLP (formyl-meth-leu-phe)-stimulated leukocytes in patients with asthma and hypersensitivity to aspirin]. / Wplyw aspiryny na chemiluminescencje leukocytów stymulowanych FMLP (formyl-meth-leu-phe) u chorych na astme z nadwrazliwoscia na aspiryne.

In order to determine if aspirin sensitivity effects leukocyte reaction in aspirin sensitive asthma patients the authors studied the influence of aspirin on chemiluminescence in 12 aspirin sensitive asthmatics and 13 non-aspirin asthmatics. Leukocyte suspension were carried out according to Böyum. Chemiluminescence was determined using the LKB--Wallac counter. No statistical differences in leukocyte chemiluminescence was found in both groups.

[Bacteriophage conversion as a factor modifying the intensity of phagocytosis of Staphylococcus aureus by human leukocytes]. / Konwersja fagowa jako czynnik modyfikujacy intensywnosc fagocytozy Staphylococcus aureus przez leukocyty ludzkie.

Staphylococcus aureus NCTC 8325-4 and its eight variants lysogenized with phages responsible for the synthesis of staphylococcal staphylokinase were used for the study. Influence of phage conversion of S. aureus on its interaction with human leucocytes and influence of prophage on strain susceptibility to intracellular killing by human granulocytes without opsonins were evaluated. It was found that lysogenization of the strain with the bacteriophages decreased in each case reactivity of human leucocytes for staphylococcal strain what was expressed by lower bioluminescence values and by lower percentage of intracellular killing of bacterial cells carrying prophage.

The role of plasmids in opsonin-independent Staphylococcus aureus-leukocyte interactions.

The opsonin-independent phagocytic and bactericidal activity of human polymorphonuclear leukocytes stimulated by two Staphylococcus aureus strains and their variants with differing plasmid patterns was investigated. Interactions between staphylococci and phagocytes were evaluated by chemiluminescence and intracellular killing tests. The results obtained indicate that the presence or absence of certain plasmids can modify staphylococcal phagocytosis and their susceptibility to intracellular killing. Pretreatment of the strains investigated with subinhibitory concentrations of mezlocillin and ofloxacin (10% MIC) influenced these parameters, too.