Assuntos
Galactose Oxidase/metabolismo , Glicosídeo Hidrolases/metabolismo , Animais , Bactérias/enzimologia , Configuração de Carboidratos , Sequência de Carboidratos , Bovinos , Glicoproteínas , Hexosaminidases/metabolismo , Masculino , Neuraminidase/metabolismo , Especificidade por Substrato , Suínos , Testículo/enzimologia , alfa-Galactosidase/metabolismo , alfa-L-Fucosidase/metabolismo , alfa-N-Acetilgalactosaminidase , beta-Galactosidase/metabolismoRESUMO
Alkaline borohydride reductive cleavage (beta-elimination) of hog submaxillary glycoproteins from three immunologically determined phenotypes, viz. A+, H+ and A-H-, resulted in the release of a series of neutral and acidic oligosaccharide-alditols. 360-MHz 1H-NMR spectroscopy in combination with methylation analysis and mass spectrometry were used for reinvestigation of the structures of these oligosaccharide-alditols. All are partial structures representing the possible complete and biosynthetically incomplete stages of the chain of a pentasaccharide-N-acetylgalactosaminitol, present in the glycoprotein with blood-group-A activity: (formula: see text) In this way, a prolonged argument about the occurrence of a NeuGc(alpha 2 leads to 6) Gal moiety in these carbohydrate chains, suggested by Aminoff et al. [Aminoff, D., Baig, M. M. and Gathmann, W. D. (1979) J. Biol. Chem. 254, 1788-1793 and 8909-8913] has been brought to a definite end. In the investigated oligosaccharide-alditols N-glycoloylneuraminic acid (NeuGc) is in no case attached to galactose (Gal), but, if present, it is (alpha 2 leads to 6)-linked to N-acetylgalactosaminitol (GalNAc-ol).
Assuntos
Mucinas/isolamento & purificação , Oligossacarídeos/isolamento & purificação , Glândula Submandibular/metabolismo , Animais , Fenômenos Químicos , Química , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Metilação , SuínosAssuntos
Oligossacarídeos , Ácidos Siálicos , Animais , Fenômenos Químicos , Química , Oxirredução , Ácido Periódico , Glândula Submandibular/análise , SuínosRESUMO
The sialic acid content of erythrocytes from healthy individuals of different blood types and of patients with known hematological disorders has been determined. The sialic acid was completely released enzymatically with sialidase and quantitated by the thiobarbituric acid method. The sialic acid content of erythrocytes was constant irrespective of ABO blood type, or anticoagulant used; viz, 0.85-0.92 mumoles/ml of packed erythrocytes or 46-53 X 10(6) sialyl residues per cell. Deviations from these normal values were obtained with erythrocytes from patients with a variety of hematological disorders. Patients with the following disorders have significantly (P less than 0.01) lower sialic acid values compared to erythrocytes from healthy individuals (given in the order of decreasing sialic acid content): sickle cell anemia, acute lymphocytic leukemia, chronic lymphocytic leukemia, acute myelomonocytic leukemia, non-Hodgkin lymphocytic lymphoma, chronic granulocytic leukemia, acute myelocytic leukemia, leukemia, and Hodgkin disease.
