Assuntos
Ácidos Graxos/metabolismo , Fungos/efeitos dos fármacos , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Consórcios Microbianos/efeitos dos fármacos , Nitratos/farmacologia , Compostos de Potássio/farmacologia , Microbiologia do Solo , Adaptação Fisiológica/efeitos dos fármacos , Carbono/metabolismo , Contagem de Colônia Microbiana , Ácidos Graxos/análise , Fungos/crescimento & desenvolvimento , Glucose/farmacologia , Bactérias Gram-Negativas/crescimento & desenvolvimento , Bactérias Gram-Positivas/crescimento & desenvolvimento , Consórcios Microbianos/fisiologia , Isótopos de NitrogênioRESUMO
Microbial biomass and community structure in paddy rice soil during the vegetation period of rice were estimated by analysis of their phospholipid fatty acids (PLFA), hydroxy fatty acids of lipopolysaccharides (LPS-HYFA), and phospholipid ether lipids (PLEL) directly extracted from the soil. A clear change in the composition of the community structure at different sampling periods was observed, indicated by the principal component analysis of the PLFA. A dramatic decline of ester-linked PLFA was observed in the soil samples taken at the second sampling time. In contrast to the ester-linked PLFA, the non-ester-linked PLFA composition did not change. The hydroxy fatty acids of lipopolysaccharides as well as ether lipids decreased consecutively during the observation period. Total microbial abundance was estimated to be (4.1-7.3) x 10(9) cells g(-1) soil (dry weight). About 44% account for aerobic and 32% for facultative anaerobic bacteria, and 24% for archaea, on average. According to the profile and patterns of PLFA in the soil sample, it may be suggested that the paddy soil at the August sampling period contained more abundant facultative anaerobic bacteria (ca. 36%) and archaea (ca. 37%), but the total microbial biomass was significantly lower than in the remaining sampling periods. As the plant approached maturity, the microbial community structure in the soil changed to contain more abundant Gram-negative bacteria and methanotrophs.
RESUMO
If there is a discrepancy between radiological findings and clinical state after massive trauma of the thorax, nuclear medicine examination is useful as is shown by a case report. Mostly it is possible to clarify the nature of abnormal masses in the lung by perfusion scintigraphy of the lung or/and inhalation of radionuclide. These examinations should be performed to control the further development of intrapulmonary alterations.
