Assuntos
Cotinina/análise , Saliva/química , Manejo de Espécimes/métodos , Cotinina/sangue , Cotinina/urina , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Técnicas Imunoenzimáticas , Sensibilidade e Especificidade , Fumar/metabolismo , Manejo de Espécimes/instrumentação , Poluição por Fumaça de TabacoAssuntos
Hormônio Adrenocorticotrópico/metabolismo , Diabetes Insípido/metabolismo , Endorfinas/metabolismo , Adeno-Hipófise/metabolismo , Ratos Brattleboro/metabolismo , Ratos Mutantes/metabolismo , beta-Lipotropina/metabolismo , Animais , Feminino , Masculino , Peso Molecular , Hormônios Adeno-Hipofisários/metabolismo , Pró-Opiomelanocortina , Precursores de Proteínas/metabolismo , Ratos , Ratos Endogâmicos/metabolismo , beta-EndorfinaRESUMO
The whole pituitary contents of beta-endorphin and ACTH were found to vary widely among 5 inbred strains of mice. beta-endorphin values were 2.5-fold different and ACTH values 1.5-fold. Strains low in beta-endorphin were also low in ACTH. The existence of genetic differences raises the possibility that there exist, or can be developed, strains with extremely low or high levels of these peptides that would aid research directed at elucidating the physiology of opioid peptides.
Assuntos
Hormônio Adrenocorticotrópico/metabolismo , Endorfinas/metabolismo , Hipófise/metabolismo , Especificidade da Espécie , Animais , Masculino , Camundongos , Camundongos Endogâmicos , Radioimunoensaio , beta-EndorfinaRESUMO
The ACTH-releasing activity of hypothalamic extract and rat plasma was examined with the dispersed rat pituitary cell technique of Swallow and Sayer (8). Although both plasma and serum caused ACTH release which was dose-related, stress did not enhance the ACTH releasing activity. Furthermore, separation studies of plasma using ultrafiltration and gel separation suggest that the CRF activity in plasma is associated with molecules of a molecular weight greater than 15,000.
