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2.
Am J Transplant ; 18(6): 1548-1551, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29316259

RESUMO

HHV-6A and HHV-6B are found as inherited and chromosomally integrated forms (iciHHV-6A and -6B) into all germinal and somatic cells and vertically transmitted in a Mendelian manner in about 1% of the population. They were occasionally shown to be horizontally transmitted through hematopoietic stem cell transplantation. Here, we present a clinical case of horizontal transmission of iciHHV-6A from donor to recipient through liver transplantation. Molecular analysis performed on three viral genes (7.2 kb) in the recipient and donor samples supports transmission of iciHHV-6A from the graft. Transmission was followed by reactivation, with high viral loads in several compartments. The infection was uncontrollable, leading to severe disease and death, despite antiviral treatments and the absence of resistance mutations. This case highlights the fact that physicians should be aware of the possible horizontal transmission of iciHHV-6 and its consequences in case of reactivation in immunocompromised patients.


Assuntos
Cromossomos Humanos , Herpesvirus Humano 6/genética , Transplante de Fígado , Integração Viral , Evolução Fatal , Herpesvirus Humano 6/fisiologia , Humanos , Ativação Viral
3.
Med Mal Infect ; 47(2): 83-91, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27773488

RESUMO

Human herpesviruses 6A, 6B, and 7 (HHV-6A, HHV-6B, HHV-7) are genetically related to cytomegalovirus. They belong to the Roseolovirus genus and to the Betaherpesvirinae subfamily. They infect T cells, monocytes-macrophages, epithelial cells, and central nervous system cells. These viruses are ubiquitous and are responsible for lifelong chronic infections, most often asymptomatic, in the vast majority of the general adult population. HHV-6B is responsible for exanthema subitum, which is a benign disease of infants. HHV-6A and HHV-6B also cause opportunistic infections in immunocompromised patients: encephalitis, hepatitis, bone marrow suppression, colitis, and pneumonitis. Their etiological role in chronic diseases such as multiple sclerosis, cardiomyopathy, and thyroiditis is still controversial. The pathogenicity of HHV-7 is less clear and seems to be much more restricted. Chromosomal integration of HHV-6A and HHV-6B is transmissible from parents to offspring and observed in about 1% of the general population. This integration raises the question of potential associated diseases and can be a confounding factor for the diagnosis of active infections by both viruses. The diagnosis of HHV-6A, HHV-6B, and HHV-7 infections is rather based on gene amplification (PCR), which allows for the detection and quantification of the viral genome, than on serology, which is mainly indicated in case of primary infection. Ganciclovir, foscarnet, and cidofovir inhibit the replication of HHV-6A, HHV-6B, and HHV-7. Severe infections may thus be treated but these therapeutic indications are still poorly defined.


Assuntos
Herpesvirus Humano 6 , Herpesvirus Humano 7 , Infecções por Roseolovirus , Árvores de Decisões , Humanos , Infecções por Roseolovirus/complicações , Infecções por Roseolovirus/diagnóstico , Infecções por Roseolovirus/tratamento farmacológico
4.
Andrologia ; 47(5): 531-5, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24846813

RESUMO

Human herpesvirus-6 (HHV-6) is a betaherpesvirus whose genome may integrate into human chromosomes. Chromosomally integrated HHV-6 (ciHHV-6) may be transmitted vertically from parents to children. HHV-6 DNA has been detected in semen, but its integrated or extrachromosomal status has not yet been characterised. The aim of this study was to determine the prevalence of HHV-6 DNA and to search for ciHHV-6 forms in spermatozoa purified from semen obtained from subjects explored for low fertility. A total of 184 sperm samples were purified using PureSperm(®) . HHV-6 viral load and species identification were performed by real-time polymerase chain reaction. Of 179 sperm specimens analysed, three were positive for HHV-6 (1.7%). Two samples (1.1%) had viral loads of 680 232 and 2 834 075 copies per million spermatozoa, compatible with loads expected for a ciHHV-6 form. The viral load of the third positive sample (73 684 copies per million spermatozoa) was lower than would be expected for ciHHV-6 infection, implying that the HHV-6 DNA detected in spermatozoa corresponds mainly to ciHHV-6. However, viral DNA may also be detected at a low level that is not in favour of the presence of ciHHV-6. Further studies are necessary to determine the origin of detected viral genomes.


Assuntos
Cromossomos Humanos/genética , DNA Viral/metabolismo , Genoma Viral/genética , Herpesvirus Humano 6/genética , Infertilidade Masculina/virologia , Infecções por Roseolovirus/epidemiologia , Sêmen/metabolismo , Espermatozoides/metabolismo , Integração Viral/genética , Humanos , Masculino , Prevalência , Reação em Cadeia da Polimerase em Tempo Real , Sêmen/virologia , Espermatozoides/virologia , Carga Viral
5.
Rev Med Interne ; 34(6): 373-6, 2013 Jun.
Artigo em Francês | MEDLINE | ID: mdl-23200797

RESUMO

INTRODUCTION: Histiocytic necrotizing lymphadenitis (Kikuchi-Fujimoto disease) is a rare clinical entity characterized by the association of enlarged lymph nodes in the posterior cervical region and fever. The disease is more frequent in young women. CASE REPORT: We report a 41-year-old African patient who presented with atypical features of Kikuchi's disease including cutaneous lupus, haemophagocytosis, and lymphocytic meningitis. The ethnic origin and the clinical presentation were initially suggestive of tuberculous meningitis. However, microbiological analyses remained negative, histological findings were suggestive of Kikuchi's disease and HHV6 DNA integration was documented in our patient. CONCLUSION: Kikuchi's disease should be suspected in an African patient when lymphocytic meningitis is associated with enlarged cervical lymph nodes, hemophagocytosis and HHV6 DNA integration.


Assuntos
Linfadenite Histiocítica Necrosante/diagnóstico , Adulto , População Negra , Diagnóstico Diferencial , Linfadenite Histiocítica Necrosante/patologia , Humanos , Linfonodos/patologia , Masculino , Pescoço
6.
Pathol Biol (Paris) ; 58(2): 166-9, 2010 Apr.
Artigo em Francês | MEDLINE | ID: mdl-19892483

RESUMO

OBJECTIVE: The Roche LightCycler 480 (LC480) system was evaluated for quantitative molecular diagnosis of opportunistic viral infections caused by human cytomegalovirus (CMV), Epstein-Barr virus (EBV), human herpesvirus 6 (HHV-6), and BK virus (BKV), in comparison with "in-house" real-time PCR assays. PATIENTS AND METHODS: A total of 253 whole blood specimens obtained from transplant recipients were tested. RESULTS: Both the "in-house" and the LC480 methods were highly correlated (Spearman correlation coefficient Rho> or =0.85; p<0.0001) with an excellent overall qualitative agreement (90.5%) and no significant quantitative difference between both techniques for the four viruses tested. The accuracy of the LC480 protocols were confirmed further by the results obtained with the 44 samples from the Quality Control for Molecular Diagnosis (QCMD) 2008 proficiency panel. CONCLUSION: The LC480 system constitutes a suitable and versatile real-time PCR platform in a routine laboratory setting for the diagnosis and monitoring of opportunistic viral infections in transplant recipients, by measuring HCMV, EBV, HHV-6, and BKV loads in whole blood samples.


Assuntos
Vírus BK/isolamento & purificação , Sistemas Computacionais , DNA Viral/sangue , Infecções por Herpesviridae/virologia , Herpesviridae/isolamento & purificação , Reação em Cadeia da Polimerase/métodos , Infecções por Polyomavirus/virologia , Kit de Reagentes para Diagnóstico , Infecções Tumorais por Vírus/virologia , Carga Viral , Viremia/virologia , Vírus BK/genética , Herpesviridae/genética , Infecções por Herpesviridae/sangue , Humanos , Transplante de Órgãos , Infecções por Polyomavirus/sangue , Complicações Pós-Operatórias/sangue , Complicações Pós-Operatórias/virologia , Controle de Qualidade , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Infecções Tumorais por Vírus/sangue
7.
J Clin Microbiol ; 47(9): 2906-11, 2009 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-19625478

RESUMO

Human immunodeficiency viruses (HIV) have a high level of genetic diversity. The outlier variants of HIV type 1 (HIV-1) group O are distantly related to HIV-1 group M. Their divergence has an impact on serological diagnosis, with a risk of false-negative results. In this study, we report 20 failure cases, involving patients with primary or chronic infection, in France and Cameroon between 2001 and 2008. Our results indicate that some assays detected group O infection much less efficiently than others. Two major reasons for these false-negative results were identified: the presence or absence of a group O-specific antigen (and the designed sequence) for the detection of antibodies and the greater envelope variability of group O than of group M strains. This study highlights the complexity of screening for these divergent variants and the need to evaluate test performance with a large panel of strains, due to the extensive diversity of group O variants.


Assuntos
Reações Falso-Negativas , Anticorpos Anti-HIV/sangue , Antígenos HIV/sangue , Infecções por HIV/diagnóstico , HIV-1/classificação , HIV-1/imunologia , Testes Sorológicos/métodos , Camarões , Feminino , França , Variação Genética , Humanos , Masculino
8.
Am J Transplant ; 9(7): 1690-7, 2009 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-19519818

RESUMO

The unique phenomenon of human herpesvirus-6 (HHV-6) chromosomal integration (CIHHV-6) may account for clinical drawbacks in transplant setting, being misinterpreted as active infection and leading to unnecessary and potentially harmful treatments. We have investigated the prevalence of CIHHV-6 in 205 consecutive solid organ (SO) and allogeneic stem cell transplant (alloSCT) Italian patients. Fifty-two (38.5%) of 135 solid organ transplant (SOT) and 16 (22.8%) of 70 alloSCT patients resulted positive for plasma HHV-6 DNA by real-time polymerase chain reaction. Seven SOT and three alloSCT patients presented HHV-6-related diseases, requiring antivirals. Two further patients (0.9%) were identified, presenting high HHV-6 loads. The quantification of HHV-6 on hair follicles disclosed the integrated state, allowing the discontinuation of antivirals. Before starting specific treatments, CIHHV-6 should be excluded in transplant patients with HHV-6 viremia by the comparison of HHV-6 loads on different fluids and tissues. Pretransplantation screening of donors and recipients may further prevent the misdiagnosis of CIHHV-6.


Assuntos
Herpesvirus Humano 6/genética , Herpesvirus Humano 6/patogenicidade , Transplante de Células-Tronco , Transplantes , Integração Viral/genética , Adulto , Estudos de Coortes , DNA Viral/sangue , DNA Viral/genética , Herpesvirus Humano 6/isolamento & purificação , Herpesvirus Humano 6/fisiologia , Humanos , Itália , Masculino , Pessoa de Meia-Idade , Infecções por Roseolovirus/diagnóstico , Infecções por Roseolovirus/etiologia , Infecções por Roseolovirus/virologia , Transplante de Células-Tronco/efeitos adversos , Transplante Homólogo , Transplantes/efeitos adversos , Viremia/diagnóstico , Viremia/etiologia , Viremia/virologia
9.
J Clin Microbiol ; 47(3): 533-40, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19109460

RESUMO

The herpes simplex virus type 1 (HSV-1) genome is a linear double-stranded DNA of 152 kpb. It is divided into long and short regions of unique sequences termed U(L) and U(S), respectively, and these are flanked by regions of inverted internal and terminal repeats. Microsatellites are short tandem repeats of 1- to 6-nucleotide motifs; they are often highly variable and polymorphic within the genome, which raises the question of whether they may be used as molecular markers for the precise differentiation of HSV-1 strains. In this study, 79 different microsatellites (mono-, di-, and trinucleotide repeats) in the HSV-1 complete genome were identified by in silico analysis. Among those microsatellites, 45 were found to be distributed in intergenic or noncoding inverted repeat regions, while 34 were in open reading frames. Length polymorphism analysis of the PCR products was used to investigate a set of 12 distinct HSV-1 strains and allowed the identification of 23 polymorphic and 6 monomorphic microsatellites, including two polymorphic trinucleotide repeats (CGT and GGA) within the UL46 and US4 genes, respectively. A multiplex PCR method that amplified 10 polymorphic microsatellites was then developed for the rapid and accurate genetic characterization of HSV-1 strains. Each HSV-1 strain was characterized by its own microsatellite haplotype, which proved to be stable over time in cell culture. This relevant innovative tool was successfully applied both to confirm the close relationship between sequential HSV-1 isolates collected from patients with multiple recurrent infections and to investigate putative nosocomial infections.


Assuntos
DNA Viral/genética , Herpesvirus Humano 1/classificação , Herpesvirus Humano 1/genética , Repetições de Microssatélites , Reação em Cadeia da Polimerase/métodos , Polimorfismo Genético , Sequência de Aminoácidos , Animais , Sequência de Bases , Chlorocebus aethiops , Análise por Conglomerados , Impressões Digitais de DNA , Genótipo , Haplótipos , Humanos , Dados de Sequência Molecular , Células Vero
10.
Virologie (Montrouge) ; 13(3): 171-179, 2009 Jun 01.
Artigo em Francês | MEDLINE | ID: mdl-36151670

RESUMO

Kits for the detection of HIV infection are in vitro-diagnostic medical devices and are subject to special regulation to be marketed in the European Community. Before 1998, the Agence française de sécurité sanitaire des produits de santé was responsible for evaluating the new kits before allowing their marketing in France. The publication in the Official Journal of the European Community (L331 of December 7, 1998) of the European directive 98/79/CE of October 27, 1998 on in vitro diagnostics medical devices has changed the procedure for marketing approval of these kits in Europe. The aim of this review is to present the relevant conformity assessment procedures and its latest updates.

11.
J Virol Methods ; 149(2): 285-91, 2008 May.
Artigo em Inglês | MEDLINE | ID: mdl-18329730

RESUMO

Human herpesviruses 6 (HHV-6), 7 (HHV-7) and 8 (HHV-8) are lymphotropic herpesviruses that may cause opportunistic diseases in immunosuppressed patients such as transplant or AIDS patients. The new commercial CMV HHV-6, 7, 8 R-gene kit (Argene, Varilhes, France) for the simultaneous quantitation of HHV-6 and qualitative detection of HHV-7 and HHV-8 was evaluated using whole blood samples (respectively, n=175, 100 and 161) and using different extraction and real-time PCR platforms in two Centers A and B. In comparison with HHV-6 in-house real-time PCR the commercial kit showed agreements of 96% (n=75) and 85% (n=100) in A and B, respectively, with significant Spearman's correlation between both techniques (in A: r=0.97 [p<0.001]; in B: r=0.70 [p<0.001]). The Bland-Altman test results and prospective monitoring of patients confirmed the accuracy of these HHV-6 real-time PCR techniques. The agreement between the in-house HHV-7 PCR and commercial kit was of 86% (n=100). In comparison with in-house HHV-8 real-time PCRs, the commercial kit showed agreements of 100% (n=61) and 93.7% (n=96) in A and B, respectively. These results demonstrate that the new commercial CMV HHV-6, 7, 8 R-gene kit was an efficient and reliable tool for the diagnosis of herpesvirus 6, 7, 8 infections.


Assuntos
Sangue/virologia , Herpesvirus Humano 6/isolamento & purificação , Herpesvirus Humano 7/isolamento & purificação , Herpesvirus Humano 8/isolamento & purificação , Reação em Cadeia da Polimerase/métodos , Infecções por Herpesviridae/diagnóstico , Herpesvirus Humano 6/genética , Herpesvirus Humano 7/genética , Herpesvirus Humano 8/genética , Humanos , Infecções por Roseolovirus/diagnóstico , Sensibilidade e Especificidade
13.
Transplantation ; 69(8): 1722-3, 2000 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-10836387

RESUMO

BACKGROUND: Human herpesviruses (HHVs) 6 and 7 are recently discovered betaherpesviruses. Although HHV-6 has been associated with disordered hematopoiesis in bone marrow transplant recipients, little information is available on the presence of both viruses in the bone marrow from healthy subjects. METHODS: We detected HHV-6 and HHV-7 DNA by means of polymerase chain reaction in bone marrow and peripheral blood samples from 18 healthy subjects who underwent total hip arthroplasty. RESULTS: Genomic HHV-6 and HHV-7 DNA were detected in 11% and 67% of the blood samples, respectively, and in 28% and 50% of the bone marrow samples, respectively. CONCLUSIONS: Both viruses may be present in the bone marrow without hematopoiesis disorder and can be transmitted through bone marrow infusion. Therefore, the causative role of these two viruses in some bone marrow diseases cannot be inferred simply from the detection of their genome in bone marrow by means of polymerase chain reaction.


Assuntos
Medula Óssea/virologia , Herpesvirus Humano 6/isolamento & purificação , Herpesvirus Humano 7/isolamento & purificação , Adulto , Idoso , Artroplastia de Quadril , Medula Óssea/química , DNA Viral/análise , DNA Viral/sangue , Feminino , Genoma Viral , Herpesvirus Humano 6/genética , Herpesvirus Humano 7/genética , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Valores de Referência
14.
Cytometry ; 40(2): 135-40, 2000 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-10805933

RESUMO

BACKGROUND: The emergence of human herpesvirus 6 (HHV-6) as a human pathogen led to the possibility of specific therapy against HHV-6 and the development of standardized susceptibility assays of HHV-6 to antivirals. METHODS: We have developed a flow cytometry method to analyze the multiplication of the HST strain of human herpesvirus 6 (HHV-6) variant B in vitro using monoclonal antibodies specific to virus proteins. This method was subsequently used to determine the sensitivity of HST multiplication in MT4 cells to four antiviral compounds of three different classes: acyclovir (ACV) and ganciclovir (GCV), two acyclic guanosine analogs; cedofovir (CDV), an acyclic nucleoside phosphonate; and phosphonoformic acid (PFA), a pyrophosphate analog. RESULTS: The 50% inhibitory concentrations (IC(50)) of ACV, GCV, CDV, and PFA determined by flow cytometry assay were 25.3, 6.4, 0.95, and 6.0 microM, respectively (5.7, 1.6, 0.3, and 1.8 microg/ml, respectively). These data together with the results of cytotoxicity assays confirmed the high efficiency and selectivity of CDV and PFA against HHV-6 B in vitro, suggested by previous results. CONCLUSIONS: Our flow cytometric assay appeared as a reproducible specific method to characterize HHV-6 susceptibility to antiviral compounds. It can be considered as a convenient alternative to the other immunologic and DNA hybridization assays used for that purpose.


Assuntos
Antivirais/farmacologia , Citosina/análogos & derivados , Infecções por Herpesviridae/tratamento farmacológico , Herpesvirus Humano 6/crescimento & desenvolvimento , Leucócitos Mononucleares/citologia , Leucócitos Mononucleares/virologia , Organofosfonatos , Compostos Organofosforados/farmacologia , Anticorpos Monoclonais , Antígenos de Superfície/análise , Antígenos de Superfície/imunologia , Antígenos Virais/análise , Antígenos Virais/imunologia , Cidofovir , Citosina/farmacologia , Relação Dose-Resposta a Droga , Citometria de Fluxo/métodos , Citometria de Fluxo/normas , Foscarnet/farmacologia , Ganciclovir/farmacologia , Herpesvirus Humano 6/efeitos dos fármacos , Herpesvirus Humano 6/imunologia , Humanos , Técnicas In Vitro , Reprodutibilidade dos Testes
15.
J Virol ; 73(11): 9655-8, 1999 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-10516079

RESUMO

We had previously described six distinct alleles of the glycoprotein B (gB) gene of human herpesvirus 7 (HHV-7). The genetic changes corresponding to these alleles did not affect gB gene transcription or translation in in vitro assays. The study of distinct HHV-7-positive human samples showed preferential associations of some gB alleles with some alleles of two other genes, distantly located on the HHV-7 genome, coding for the phosphoprotein p100 (p100) and the major capsid protein (MCP). Two allele combinations, corresponding to 44 and 31% of the samples studied, respectively, were interpreted as the genetic signatures of two major prototype HHV-7 variants.


Assuntos
Alelos , Genes Virais , Variação Genética , Infecções por Herpesviridae/virologia , Herpesvirus Humano 7/genética , Capsídeo/genética , Humanos , Fosfoproteínas/genética , Polimorfismo Genético , Biossíntese de Proteínas , Transcrição Gênica , Proteínas do Envelope Viral/genética
17.
J Virol ; 72(11): 8725-30, 1998 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-9765415

RESUMO

As for other herpesviruses, glycoprotein B (gB) of human herpesvirus 7 (HHV-7) is believed to play a major role in virus infection and as a target of the host immunogenic response. Using nested PCR, we amplified the whole HHV-7 gB gene from 108 human peripheral blood mononuclear cell samples and studied its variability. By means of restriction fragment length polymorphism (RFLP) analysis, three distinct patterns, designated I, II, and III, were defined and detected at frequencies of 93, 5, and 2%, respectively. Determination of the nucleotide sequence allowed us to recognize five critical positions in the gB gene with six specific combinations of point changes at these positions. These combinations were gB alleles A, B, C, D, E, and F. Alleles D and E corresponded to RFLP patterns II and III, respectively, while the other four alleles corresponded to RFLP pattern I. Identical gB alleles were detected in serial samples as well as in paired samples of blood and saliva from the same individuals, except for one case. In contrast, the distribution of gB alleles differed according to the geographical origin of the human samples: C was the most frequent allele in both African and Caribbean samples, whereas F was the most frequent allele in European ones. Although none of the allele-specific nucleotide changes induced any modification at the protein level, the definition of gB alleles provided convenient viral markers for the study of both HHV-7 infections and human population genetics.


Assuntos
Genes Virais , Herpesvirus Humano 7/genética , Proteínas do Envelope Viral/genética , África/epidemiologia , Alelos , Sequência de Bases , Primers do DNA/genética , DNA Viral/genética , Europa (Continente)/epidemiologia , Genética Populacional , Infecções por Herpesviridae/epidemiologia , Infecções por Herpesviridae/virologia , Herpesvirus Humano 7/isolamento & purificação , Humanos , Leucócitos Mononucleares/virologia , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Saliva/virologia , Índias Ocidentais/epidemiologia
18.
Transplantation ; 65(10): 1408-11, 1998 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-9625030

RESUMO

BACKGROUND: Human herpesvirus-6 (HHV-6) has been implicated in bone marrow suppression, interstitial pneumonitis, and fatal meningoencephalitis in bone marrow transplant (BMT) recipients. METHODS: We describe the case of a woman with acute myeloid leukemia in second remission who developed febrile meningoencephalitis 8 months after a second unrelated BMT. RESULTS: Computed tomography and magnetic resonance images of the brain were nonspecific. Analysis of cerebrospinal fluid (CSF) revealed lymphocytosis and an increased protein level. Using polymerase chain reaction methods, HHV-6 was the only pathogen detected in CSF, peripheral blood mononuclear cells, and bone marrow. The patient was treated with ganciclovir and foscarnet for 3 months. All clinical manifestations resolved and HHV-6 polymerase chain reaction analysis of CSF became negative 40 days after the beginning of antiviral treatment. CONCLUSIONS: This observation strongly suggests that HHV-6 should be sought in BMT patients with neurological complications and that HHV-6 meningoencephalitis may respond to ganciclovir and foscarnet therapy.


Assuntos
Transplante de Medula Óssea , Infecções por Herpesviridae , Herpesvirus Humano 6 , Complicações Pós-Operatórias , Doença Aguda , Adulto , Antivirais/uso terapêutico , Feminino , Foscarnet/uso terapêutico , Ganciclovir/uso terapêutico , Infecções por Herpesviridae/tratamento farmacológico , Humanos , Leucemia Mieloide/cirurgia , Transplante Homólogo
19.
J Clin Microbiol ; 35(6): 1600-3, 1997 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-9163493

RESUMO

Human cytomegalovirus (HCMV) is a well-known opportunistic agent that reactivates in human immunodeficiency virus (HIV)-seropositive subjects. Human herpesvirus 6 (HHV-6) and HHV-7 were discovered recently and, like HCMV, belong to the Betaherpesvirinae subfamily. We looked for the presence of HCMV, HHV-6, and HHV-7 by PCR with saliva and urine samples from 125 HIV-seropositive patients at different stages of HIV infection and with saliva and urine samples from 29 HIV-seronegative subjects. All three viruses were frequently detected in the saliva (overall rates of detection, 61, 43, and 63% for HCMV, HHV-6, and HHV-7, respectively) with no correlation with the stage of immune deficiency. In contrast, HCMV was detected in urine much more frequently than the two other herpesviruses (overall rates of detection, 37, 2, and 6.5% for HCMV, HHV-6, and HHV-7, respectively) and was associated with immune deficiency. This suggests that these three genetically related viruses differ from each other with regard to replication in the urinary tract.


Assuntos
Infecções Oportunistas Relacionadas com a AIDS/virologia , Betaherpesvirinae/isolamento & purificação , Infecções por Herpesviridae/virologia , Herpesvirus Humano 7/isolamento & purificação , Hospedeiro Imunocomprometido , Contagem de Linfócito CD4 , DNA Viral/urina , Soropositividade para HIV/imunologia , Soropositividade para HIV/virologia , Humanos , Saliva/virologia
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