[Sensitization of lymphocytes from newborn infants and young children to pathogenic and opportunistic microflora]. / O sensibilizatsii limfotsitov novorozhdennykh i detei rannego vozrasta k patogennoi i uslovno-patogennoi mikroflore.

The nucleolar test was used to study sensitization of peripheral blood lymphocytes from the newborn and infants. The test is based on the counting of cells with a clarification area around nucleoli after incubation with antigens and microbial bodies of the pathogenic and opportunistic microflora. It has been established that the peripheral blood from children with infectious-inflammatory diseases and from those vaccinated with BCG shows an increase in the sensitized lymphocyte count which is influenced by child's trophicity at birth and by administration of antibacterial therapy. The use of the nucleolar test as a diagnostic aid is advisable to identify the disease etiology.

[Differential microcalorimetry of the antigen-antibody reaction]. / Differentsial'naia mikrokalorimetriia reaktsii antigen-antitelo.

High sensitivity of the microcalorimetric method for the registration of immunologic reactions and prospects of the practical application of the method demand an objective interpretation of the results and an explanation of the range of thermistometry findings varying from positive to negative values. The investigations have been carried out with models with known titers of purified antigens and antibodies and with unknown antibody titers. The method has been employed in examinations of children suffering from infectious allergic diseases and from central nervous system abnormalities. The energy processes connected with the negative, zero, or positive results of the reactions, depending on the antibody and antigen concentrations, are explained.

[Morphologic changes of the nucleolar system of lymphocytes as the indicator of sensitivity to tuberculin and Toxoplasma antigen]. / Morfologicheskie izmeneniia nukleoliarnogo apparata limfotsitov kak ppkazate'l chuvstvite'lnosti k tuberkulinu i toksoplazmen- nomu antigenu.

A study was made of the nucleolar apparatus of peripheral blood lymphocytes of the newborns, infants and their mothers before and after incubation in vitro with tuberculin or toxoplasmic antigen. It was established that in the presence of sensitization to one or another antigen, there was an increase in the relative count of lymphocytes having a zone of clarification near nucleoli. In children vaccinated with BCG, such an effect was noted in 81.3% of the cases, whereas in children with verified toxoplasmosis, in all the cases. It is recommended that the method designed might be used as alternative for the complement fixation test in the diagnosis of toxoplasmosis.