[Quantitative objective and subjective assessment of the quality of treatment of patients with distal occlusion]. / Kolichestvennaya ob"ektivnaya i sub"ektivnaya otsenka kachestva lecheniya patsientov s distal'noi okklyuziei zubnykh ryadov.

OBJECTIVE: The aim of the study is to evaluation of the quality of orthodontic treatment by comparing the severity of signs of distal occlusion before the start of orthodontic treatment and after its completion. MATERIAL AND METHODS: Quantitative assessment of the severity of distal occlusion in 83 patients aged 17 to 30 years before and after orthodontic treatment. Depending on the chosen treatment algorithms, the patients were divided into 3 groups. The first group - 34 people - treatment included the use of a functional non-removable orthodontic device (FNT). The second group - 21 people - the treatment was accompanied by the removal of individual teeth of the upper jaw and the retraction of a group of front teeth of the upper jaw. Patients of the 3rd group - 28 people - underwent combined orthodonto-surgical treatment. The severity of distal occlusion was assessed using 3D cephalometric analysis data. To obtain a subjective assessment of the quality of orthodontic treatment, patients were asked to fill out questionnaires before and after orthodontic treatment. RESULTS: The severity of distal occlusion decreased as much as possible as a result of treatment of the first group in 23.07% of patients and the third group (16.63%), which is interpreted by the formulation «significant improvement¼ of the dental anomaly. Patients of the first and third groups had a high degree of satisfaction with the result of treatment, according to the quantitative assessment of the questionnaire. The severity of distal occlusion in the second group was reduced to a lesser extent (55.5%), compared with the first and third groups, which is interpreted as «moderately improved¼. Patients noted an average degree of satisfaction with the result of treatment, as the implemented treatment plan did not meet their expectations in the aesthetic aspect. CONCLUSION: The use of FNT for the extension and normalization of the position of the lower jaw is justified in the case of refusal of patients from combined orthodonto-surgical treatment, as it allows to fully solve the functional, aesthetic and psychological problems of patients, which contributes to improving their quality of life.

[Orthodontic treatment of adult patients with mandibular retrognathia]. / Ortodonticheskoe lechenie vzroslykh patsientov s nizhnei retrognatiei.

This article describes a case report of the patient with mandibular retrognathia, class II malocclusion, constriction and deformation of dental arches and bimaxillary protrusion. Due to the patient's refuse to undergo the orthognathic surgery, after diagnostic, it was decided to carry out the orthodontic dentoalveolar compensation. The combined use of the functional fixed telescopic appliance (FFTA), bracket system and orthodontic miniscrews made it possible to effectively normalize the mandibular position, achieve orthognathic occlusion, eliminate bimaxillary protrusion and improve the face profile. This method significantly reduced invasiveness and time of orthodontic treatment.

[Objective and subjective assessment of the quality and effectiveness of orthodontic treatment]. / Ob"ektivnaya i sub"ektivnaya otsenka kachestva i effektivnosti ortodonticheskogo lecheniya.

Dentofacial anomalies occupy one of the leading places among dental diseases in people of different age groups. Analysis of epidemiological data on the prevalence of dentoalveolar anomalies indicates that there is no tendency to decrease it in Russia and abroad countries. The demand for orthodontic treatment is increasing with the development of new technologies and materials, the development of modern methods of treatment in the field of dentistry. This trend is accompanied by increased requirements for the quality of orthodontic care. The patient's dissatisfaction with the result of treatment most often arises because of a discrepancy between his subjective assessment of treatment and the objective anatomical, functional and aesthetic state of occlusion. The use of methods for quantitative assessment of dentoalveolar anomalies and the quality of orthodontic treatment makes it possible to objectively substantiate the choice of tactics of orthodontic care and increase its effectiveness. At the same time, the patient's subjective assessment of the quality of orthodontic treatment allows one to see how the proposed (implemented) treatment plan reflects the patient's main complaints and to assess the degree of his satisfaction with the achieved result. That`s why, the issue of using methods of objective and subjective assessment of the quality and effectiveness of orthodontic treatment is relevant today. The purpose of the literature review is to consider the development and implementation of criteria for an objective and subjective assessment of the outcome of orthodontic treatment.

[Assessment of dental arches expansion using ligature and passive self-ligating braces in combination with Pitts broad arches and early elastics]. / Otsenka rasshireniya zuboal'veolyarnykh dug u patsientov pri ispol'zovanii ligaturnykh i passivnykh samoligiruyushchikh breket-sistem v sochetanii s dugami Pittsa shirokoi formy i rannei elastichnoi tyagi.

THE AIM OF THE STUDY: Was to analyze the effectiveness of the use of Pitts Broad and early elastics in the treatment of patients with narrowing and deformation in dentition. MATERIALS AND METHODS: The results of orthodontic treatment of 46 adult patients with narrowing and deformation in dentition are presented. Patients were separated in 2 groups, depending on the algorithm of treatment: group 1 - using the braces of passive self-ligation, Pitts Broad and early short elastics; group 2 - using ligature braces and archwires Orthos. RESULTS: Biometric analysis of the diagnostic models of the 1st group after treatment showed a significant increase in the area of the premolars on the upper (between premolars - 39.8±0.4 mm, between molars - 48.3±0.5 mm) and lower (between premolars - 37.1±0.5 mm, between molars - 46.3±0.6 mm) jaws compared to the data before treatment. Biometric analysis of the diagnostic models of the 2d group showed that expansion in the area of the molars was achieved in a much smaller volume in the upper and lower jaws, compared with the 1st group (upper jaw: between premolars - 35.7±0.4 mm, between molars - 43.5±0.5 mm; lower jaw: between premolars - 33.2±0.3 mm, between molars - 42.3±0.5 mm). CONCLUSION: The passive self-ligation system in combination with protocol of Pitts Broad already contributes the expansion of dentition in aesthetically significant areas (distal regions) in the early stages of treatment. Using of early light elastics provides an early control of the lateral tooth torque, which allows achieving a harmonious and wide smile at the end of treatment.

[Algorithm for treatment of patients with class II malocclusion rejecting orthognathic surgery]. / Algoritm lecheniya patsientov s nizhnei retrognatiei, otkazavshikhsya ot kostno-rekonstruktivnoi operatsii.

The aim of this study is to develop algorithms for the treatment of patients with class II malocclusion and incisor protrusion using the combination of bracket system, functional fixed telescopic appliance (FFTA) and skeletal anchorage, which allows to achieve effective dentoalveolar compensation of skeletal forms of class II malocclusion. MATERIALS AND METHODS: Orthodontic treatment of 60 patients with class II malocclusion at the age of 18 to 44 years, using the bracket system, FFTA and orthodontic miniscrews was performed. In order to assess the results of treatment, cone-beam computed tomography were obtained at the beginning and at the end of treatment. RESULTS: As a result of the treatment normalization of lower jaw position, dental position and occlusion were achieved, the lower incisors protrusion was eliminated and the facial profile was improved, reliably confirmed by lateral cephalograms. CONCLUSION: The proposed treatment algorithm proved to be effective for stable correction of skeletal class II malocclusion in patients rejecting orthognathic surgery.

Microneedle patch based on dissolving, detachable microneedle technology for improved skin quality of the periorbital region. Part 2: Clinical Evaluation.

OBJECTIVE: Microneedle patches based on dissolving, detachable microneedle technology (Russian patent No. 2652567; US patent EFS No. 32735812; WO/2019/231360) are novel dermatological products that allow safe, painless and effective reduction of epidermal wrinkles after six procedures. The purpose of this study was two-fold (a) to assess the safety and efficacy of microneedle patches comprising 650 microneedles containing hyaluronic and ferulic acids after 6 procedures of applying the applicator to the skin for 25 min; and (b) to correlate our previously reported ex vivo findings. METHODS: The microneedle applicator contains 650 microneedles, which dissolve in 25 min of exposure. The effectiveness of microneedle applicators was confirmed by a randomized split-phase study involving 82 subjects. Applications of microneedle patches were performed at intervals of two times per week, and the effectiveness was assessed at 6 weeks after the start of the study. RESULTS: The results of the profilometric evaluation of skin smoothness demonstrated a significant reduction in the average roughness index by 65.32 ± 2.99% and in the mean skin waviness by 66.84 ± 1.6% compared with these indicators in the control group (P < 0.05). The therapeutic effect of the procedure was confirmed by an ultrasound examination that registered a 72.2 ± 5.4% and 25 ± 1.4% increase in echo-density of the epidermis and dermis, respectively. An independent blinded skin evaluation by dermatologists revealed steady decrease in puffiness of the application area in 89.9% of patients, increased elasticity in 78.3% of the patients, and reduced severity of epidermal wrinkles in 89.9% of the patients. CONCLUSIONS: The applicator with 650 soluble microneedles on its surface containing hyaluronic and ferulic acids is considered a safe, effective and convenient way to improve the skin quality in the periorbital region after six procedures. Nevertheless, additional studies of soluble microneedles are required to fully assess the amount and distribution area of the injected hyaluronic acid and other active components, as well as to detail the mechanism of action of soluble microneedles to improve skin quality.

OBJECTIF: Les patchs Microneedle basés sur la technologie de micro-aiguille dissolvante et détachable (brevet russe n ° 2652567; brevet américain EFS n ° 32735812; WO / 2019/231360) sont de nouveaux produits dermatologiques qui permettent une réduction sûre, indolore et efficace des rides épidermiques après 6 procédures. Le but de cette étude était double: a) évaluer l'innocuité et l'efficacité des patchs de micro-aiguilles comprenant 650 micro-aiguilles contenant des acides hyaluronique et férulique après 6 procédures d'application de l'applicateur sur la peau pendant 25 minutes; et b) pour corréler nos résultats ex-vivo précédemment rapportés. MÉTHODES: L'applicateur de micro-aiguilles contient 650 micro-aiguilles, qui se dissolvent en 25 minutes d'exposition. L'efficacité des applicateurs de micro-aiguilles a été confirmée par une étude randomisée en hémi-visage portant sur 82 sujets. Des applications de patchs de micro-aiguilles ont été effectuées à des intervalles de 2 fois par semaine, et l'efficacité a été évaluée 6 semaines après le début de l'étude. RÉSULTATS: Les résultats de l'évaluation profilométrique de la douceur de la peau ont démontré une réduction significative de l'indice de rugosité moyen de 65,32 ± 2,99% et de l'ondulation cutanée moyenne de 66,84 ± 1,6% par rapport à ces indicateurs dans le groupe témoin (p <0,05). L'effet thérapeutique de la procédure a été confirmé par un examen échographique qui a enregistré une augmentation de 72,2 ± 5,4% et 25 ± 1,4% de l'écho-densité de l'épiderme et du derme, respectivement. Une évaluation indépendante de la peau en aveugle par des dermatologues a révélé une diminution constante des poches de la zone d'application chez 89,9% des patients, une augmentation de l'élasticité chez 78,3% des patients et une réduction de la gravité des rides épidermiques chez 89,9% des patients. CONCLUSIONS: L'applicateur avec 650 micro-aiguilles solubles à sa surface contenant des acides hyaluronique et férulique est considéré comme un moyen sûr, efficace et pratique d'améliorer la qualité de la peau dans la région périorbitaire après 6 procédures. Néanmoins, des études supplémentaires sur les micro-aiguilles solubles sont nécessaires pour évaluer pleinement la quantité et la zone de distribution de l'acide hyaluronique injecté et d'autres composants actifs, ainsi que pour détailler le mécanisme d'action des micro-aiguilles solubles pour améliorer la qualité de la peau.

The Simultaneous Voltammetric Determination of Aflatoxins В1 and М1 on a Glassy-Carbon Electrode.

For the first time, the possibility of using stripping voltammetry for the simultaneous determination of aflatoxins В1 and М1 on a glassy-carbon electrode has been shown. The influence of various factors (Еэ, τэ, w, and the nature of the background electrolyte) on the potential and magnitude of the oxidation current of mycotoxins has been estimated. Working conditions for voltammetric determination and reproducibility of analytical signals for two mycotoxins have been selected. The mutual influence of aflatoxins В1 and М1 on the value of analytical signals in their simultaneous presence has been studied. It has been found that, in the range of their detectable contents, the presence of aflatoxin В1 reduces the analytical signal of aflatoxin М1 by 45-50%, but the linearity of the calibration dependence is preserved. The content of aflatoxin М1 in determination of aflatoxin B1 does not exert a significant effect in the range of 10-15%. Based on the results obtained, a procedure has been proposed for determining the content of aflatoxins В1 and М1 in their joint presence in milk by voltammetry in the concentration ranges 2 × 10-3 ÷ 2 × 10-1 mg/dm3 and 2 × 10-4 ÷ 2 × 10-2 mg/dm3, respectively (Sr not more than 18%).

Novel clinical and molecular findings in Spanish patients with naevoid basal cell carcinoma syndrome.

BACKGROUND: Naevoid basal cell carcinoma syndrome (NBCCS) is an autosomal dominant disorder characterized by developmental alterations and multiple basal cell carcinomas. Mutations in PTCH1, which encodes a membrane receptor for Sonic Hedgehog, are associated with the development of the disease. Most of them produce a truncated protein, which is unable to suppress Smoothened protein and continuously activates the downstream pathway. OBJECTIVES: We aimed to characterize 22 unrelated Spanish patients with NBCCS, the largest cohort with Gorlin syndrome reported to date in Spain. METHODS: Genomic analysis of PTCH1 was performed in patients with NBCCS and controls, and mutations were analysed using bioinformatics tools. RESULTS: We report for the first time two young patients, one each with uterus didelphys and ganglioneuroma, within the context of NBCCS. One patient showing a severe phenotype of the disease had developed basal cell carcinomas since childhood. Sanger sequencing of PTCH1 in this cohort identified 17 novel truncating mutations (11 frameshift, five nonsense and one mutation affecting an exon-intron splice site) and two novel missense mutations that were predicted to be pathogenic. The patients showed great clinical variability and inconsistent genotype-phenotype correlation, as seen in relatives carrying similar mutations. CONCLUSIONS: This study contributes to increase the pool of clinical manifestations of NBCCS, as well as increasing the number of pathogenic mutations identified in PTCH1 predisposing to the condition. The inconsistencies found between phenotype and genotype suggest the involvement of other modifying factors, genetic, epigenetic or environmental.

Dermatosis neglecta o terra firma-forme dermatosis / Dermatosis Neglecta or Terra Firma-Forme Dermatosis

No disponible

An algorithm for three-dimensional Voronoi S-network.

The paper presents an algorithm for calculating the three-dimensional Voronoi-Delaunay tessellation for an ensemble of spheres of different radii (additively-weighted Voronoi diagram). Data structure and output of the algorithm is oriented toward the exploration of the voids between the spheres. The main geometric construct that we develop is the Voronoi S-network (the network of vertices and edges of the Voronoi regions determined in relation to the surfaces of the spheres). General scheme of the algorithm and the key points of its realization are discussed. The principle of the algorithm is that for each determined site of the network we find its neighbor sites. Thus, starting from a known site of the network, we sequentially find the whole network. The starting site of the network is easily determined based on certain considerations. Geometric properties of ensembles of spheres of different radii are discussed, the conditions of applicability and limitations of the algorithm are indicated. The algorithm is capable of working with a wide variety of physical models, which may be represented as sets of spheres, including computer models of complex molecular systems. Emphasis was placed on the issue of increasing the efficiency of algorithm to work with large models (tens of thousands of atoms). It was demonstrated that the experimental CPU time increases linearly with the number of atoms in the system, O(n).

Role of different B-cell subsets in the specific and polyclonal immune response to T-independent antigens type 2.

Role of different B-cell subsets in the immune response to T-independent antigen type 2 (TI-2) was studied. BALB/c and C57BL/6 mice were immunized by polyvinylpyrrolidon (PVP), and the numbers of antibody- and Ig-forming cells (AFC and IFC, respectively) were determined by ELISPOT method. The number of cells producing non-specific Ig (nIFC) was calculated as the difference between the number of IFC and AFC; the number of nIFC induced by PVP was calculated as the difference between the number of nIFC in immune and control splenocytes. Immunization by PVP induced not only the AFC appearance, but also the increase in the number of the antigen-induced nIFC. The treatment of splenocytes by anti-CD5 antibodies and guinea pig complement reduced the increase in the numbers of newly formed AFC and nIFC to approximately 40% of control level. It means that CD5+ cells play an important role not only in the specific, but also in polyclonal immune response to non-self TI-2. To be sure that the decrease of AFC and nIFC numbers is due to depletion for CD5+ B-cells, but not CD5+ T-cells, splenocytes were separated to B-1 and B-2 subsets, and the numbers of AFC, IFC and nIFC were determined in each B-cell subpopulation separately. The overwhelming majority of newly formed AFC and nIFC was detected in B-1 subset. The numbers of AFC and nIFC in B-1 compartment was approximately 10-fold greater than in B-2 cells. A close parallelism between AFC and nIFC formation was observed. It is concluded that specific and polyclonal immune response to non-self TI-2-PVP-depends mainly on CD5+ B-1 subset.