Understanding the role of p38 and JNK mediated MAPK pathway in response to UV-A induced photoaging in Caenorhabditis elegans.

Premature aging of the skin, principally induced by the UV radiations is called as photoaging, characterized by an increase in the level of ROS and the damage of the collagen layer leading to the damage of the cells. Mitogen activated Protein kinase (MAPK) pathway is known to mediate photoaging by controlling the level of ROS and initiating detoxification. Caenorhabditis elegans, a known model to analyze photoaging was used to understand the role of MAPK pathway (p38 and JNK) during UV-A mediated photoaging. Gene specific mutants of p38 MAPK pathway showed reduced survival when exposed to UV-A suggesting that UV-A mediated photoaging was dependent on this pathway. Also, the role of SKN-1 in eliciting response against UV-A was analyzed with the help of GFP tagged strains and qPCR analysis. Further, UV-A did not have any impact on the lifespan of JNK pathway mutants suggesting the importance of the pathway in eliciting a response against UV-A exposure, which was further validated by Western blot analysis. Overall, this study suggests that MAPK pathway could play an important part in initiating and eliciting a response by the host against UV-A exposure, by which it could be used as a marker to analyze the effects of photoaging.

Analyzing the Synergistic Effects of Antioxidants in Combating Photoaging Using Model Nematode, Caenorhabditis elegans.

Aging, a universal and unique process, occurs both intrinsically (chronological) and extrinsically (photoaging). Ultraviolet-A (UV-A)-mediated stress is a growing health hazard to mankind as it is the major cause of photoaging, which could lead to much damage of skin cells and tissues ranging from tan, burn, or even cancer. The present study focuses on the role of antioxidants and other natural compounds which have been widely used in oral/topical applications to combat and delay the effects of photoaging using model nematode Caenorhabditis elegans. Compounds like green tea extract, naringenin, and naringin, which are known for their antioxidant properties, were able to extend life span and healthspan of the nematode in normal as well as under UV-A-mediated stress conditions. Regulation of both the stress-responsive genes (skn-1 and sir-2.1) and the aging-regulating genes (daf-2 and age-1) was attributable for these conditions. Interestingly, it was observed that these compounds when combined in equal ratios by weight worked synergistically to combat the aging process. Pronounced synergistic effects were observed during UV-A-mediated stress conditions, suggesting that these could be used as potential antiphotoaging compounds which will be of greater significance for health-based research.

In vitro study of magnesium-calcite biomineralization in the skeletal materials of the seastar Pisaster giganteus.

The mechanisms of formation of biogenic magnesium-rich calcite remain an enigma. Here we present ultrastructural and compositional details of ossicles from the seastar Pisaster giganteus (Echinodermata, Asteroidea). Powder X-ray diffraction, infrared spectroscopy and elemental analyses confirm that the ossicles are composed of magnesium-rich calcite, whilst also containing about 0.01 % (w/w) of soluble organic matrix (SOM) as an intracrystalline component. Amino acid analysis and N-terminal sequencing revealed that this mixture of intracrystalline macromolecules consists predominantly of glycine-rich polypeptides. In vitro calcium carbonate precipitation experiments indicate that the SOM accelerates the conversion of amorphous calcium carbonate (ACC) into its final crystalline product. From this observation and from the discovery of ACC in other closely related taxa, it is suggested that substitution of magnesium into the calcite lattice through a transient precursor phase may be a universal phenomenon prevalent across the phylum echinodermata.

Formation of transient amorphous calcium carbonate precursor in quail eggshell mineralization: an in vitro study.

To understand the mechanism of quail eggshell biomineralization, we have performed two CaCO(3) precipitation experiments. In the reprecipitation experiments, supersaturated Ca(HCO(3))(2) was prepared by bubbling CO(2) through a slurry of biogenic CaCO(3) obtained from bleach-treated eggshell followed by filtration to obtain a clear solution for crystallization experiments. The nucleated crystals were collected at various time intervals and analyzed. In the second experiment, the extracted SOM from the bleach-treated eggshell was added to the supersaturated clear solution of Ca(HCO(3))(2) solution obtained by bubbling CO(2) gas through a slurry of synthetic CaCO(3) followed by filtration. The crystals/precipitates collected at various time intervals were analyzed. Both experiments showed that amorphous CaCO(3) (ACC) was precipitated in the early stages, which then transformed to the most stable crystalline calcite phase. Amino acid analysis of the soluble organic matrixes (SOM) indicated the presence of high amounts of Glx and Asx amino acids. Ovomucoid--an acidic glycoprotein, and lysozyme--a basic protein, are the two major components along with a few low molecular weight peptides present in the SOM of quail eggshell matrix. Both ovomucoid and lysozyme did not induce precipitation of the ACC phase in in vitro conditions, while the fraction containing low molecular weight peptides induced the precipitation of ACC, suggesting that the latter play an important role in the eggshell biomineralization. Thus, organisms can produce inorganic minerals which assume nonequilibrium morphologies and intricate architecture by precipitating transient ACC, which then transformed into the crystalline phase. Altogether, these observations further demonstrate that this strategy may be common in both vertebrate and invertebrate mineralized structures.