Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Pestic Biochem Physiol ; 158: 12-17, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31378346

RESUMO

Lithospermum arvense is a troublesome dicotyledonous winter annual weed of wheat in China. A L. arvense population (HN01) suspected of being resistant to acetolactate synthase (ALS) inhibitors was found in Henan Province, China. This study aimed to testify the sensitivity of this HN01 population to eight herbicides from 3 different modes of action, and to explore the potential target-site-resistance mechanism to tribenuron-methyl. The whole-plant bioassays indicated that the population was highly resistant to tribenuron-methyl (SU, 350-fold), pyrithiobac sodium (PTB, 151-fold), pyroxsulam (TP, 62.7-fold), florasulam (TP, 80.6-fold), and imazethapyr (IMI, 136-fold), but was sensitive to carfentrazone-ethyl and fluroxypyr-meptyl. ALS gene sequencing revealed that the Trp (TGG) was substituted by Leu (TTG) at codon 574 in resistant plants. In in vitro ALS assays, the concentration of tribenuron-methyl required to inhibit 50% ALS activity (I50) for HN01 was 117-fold greater than that required to inhibit a susceptible population (HN05), indicating that resistance was due to reduced sensitivity of the ALS enzyme to tribenuron-methyl. To the best of our knowledge, this is the first report of ALS gene Trp-574-Leu amino acid mutation confer resistance to tribenuron-methyl in L. arvense.


Assuntos
Acetolactato Sintase/genética , Lithospermum/efeitos dos fármacos , Lithospermum/enzimologia , Mutação/genética , Sulfonatos de Arila/toxicidade , Benzoatos/toxicidade , Resistência a Herbicidas/genética , Herbicidas/toxicidade , Lithospermum/genética , Ácidos Nicotínicos/toxicidade , Proteínas de Plantas/genética , Pirimidinas/toxicidade , Sulfonamidas/toxicidade
2.
Pest Manag Sci ; 75(12): 3175-3183, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30950550

RESUMO

BACKGROUND: Asia minor bluegrass (Polypogon fugax Nees ex Steud.) is an invasive grass species severely infesting wheat and canola fields in China. In May 2017, a suspected resistant P. fugax population AHHY that survived fenoxaprop-P-ethyl applied at its field-recommended rate was collected from a wheat field in Huaiyuan County, Anhui Province, China. This study aimed to determine the resistance profile of AHHY to acetyl-CoA carboxylase (ACCase) inhibitors and to investigate its mechanisms of resistance to fenoxaprop. RESULTS: Single-dose testing indicated that the AHHY population had evolved resistance to fenoxaprop. The partial carboxyltransferase domain of ACCase in P. fugax was amplified and compared. Four loci encoding plastidic ACCase were isolated from both the resistant and sensitive individuals. Combining gene sequencing with the derived cleaved amplified polymorphic sequence assay, we found that 100% of the plants of AHHY carried Trp-1999-Ser mutation in their ACCase1,1-2 allele. Whole-plant dose-response bioassay indicated that AHHY was highly resistant to fenoxaprop and pinoxaden (resistance index (RI) ≥ 10) with low resistance to clodinafop-propargyl, sethoxydim, and clethodim (2 ≤ RI < 5). Pre-treatment with piperonyl butoxide largely reduced (55%) the weed's resistance to fenoxaprop. Both basal and fenoxaprop-induced glutathione S-transferases activities toward 1-chloro-2, 4-dinitrobenzene were significantly higher in resistant plants than in susceptible plants. CONCLUSION: This study revealed that P. fugax had multiple alleles encoding plastidic ACCase, and reported for the first time the occurrence of Trp-1999-Ser mutation and non-target-site resistance in this species. Fenoxaprop resistance in AHHY plants was conferred by target-site mutation and P450s-involved enhanced metabolism. © 2019 Society of Chemical Industry.


Assuntos
Acetil-CoA Carboxilase/genética , Sistema Enzimático do Citocromo P-450/metabolismo , Resistência a Herbicidas/genética , Herbicidas/farmacologia , Oxazóis/farmacologia , Proteínas de Plantas/genética , Poaceae/genética , Propionatos/farmacologia , Acetil-CoA Carboxilase/metabolismo , Mutação , Proteínas de Plantas/metabolismo , Poaceae/efeitos dos fármacos
3.
Pest Manag Sci ; 75(1): 204-214, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29808621

RESUMO

BACKGROUND: Shortawn foxtail (Alopecurus aequalis Sobol.) is a competitive grass weed infesting winter wheat- and canola-growing fields in China. In May 2016, a suspected A. aequalis resistant population AHTC-06 that survived fenoxaprop-P-ethyl and mesosulfuron-methyl applied at their field-recommended rates was collected from a wheat field in Jinji County, Anhui Province, China. This study aimed to determine the resistance profile of this AHTC-06 population to ACCase- and ALS-inhibitors, and to investigate its mechanisms of resistance to fenoxaprop-P-ethyl and mesosulfuron-methyl. RESULTS: Two mutations, a common Ile-2041-Asn (ACCase gene) and a very rare Pro-197-Tyr (ALS1 gene), were both identified in resistant individual plants. The homozygous subpopulation AHTC-06F1 for the two mutations was generated, and it showed broad-spectrum resistance to APPs, DENs, and ALS-inhibiting herbicides of all five chemical families tested, with resistance index (RI) values that ranged from 2.2 to 36.5. In vitro ALS activity assays showed the ALS from the resistant population was insensitive to all the tested ALS inhibitors, with RI values ranging from 3.10 to 22.51. Pre-treatment with piperonyl butoxide (PBO) and malathion significantly (P < 0.05) reversed the weed's resistance to fenoxaprop-P-ethyl and mesosulfuron-methyl, respectively. Two P450 genes, c21190_g1 and c43350_g3, were constitutively overexpressed and mesosulfuron-methyl-induced upregulated in resistant plants, for which c43350_g3 was also fenoxaprop-P-ethyl-induced upregulated. CONCLUSION: This study confirms the first case of a grass weed featuring broad-spectrum resistance to ALS-inhibiting herbicides due to a Pro-197-Tyr mutation in the ALS gene. Fenoxaprop-P-ethyl and mesosulfuron-methyl resistances in AHTC-06 plants were conferred by target site mutations and P450s-based metabolism. © 2018 Society of Chemical Industry.


Assuntos
Sistema Enzimático do Citocromo P-450/genética , Resistência a Herbicidas/genética , Herbicidas/farmacologia , Oxazóis/farmacologia , Proteínas de Plantas/genética , Poaceae/genética , Propionatos/farmacologia , Compostos de Sulfonilureia/farmacologia , Sistema Enzimático do Citocromo P-450/metabolismo , Mutação , Proteínas de Plantas/metabolismo , Poaceae/efeitos dos fármacos , Poaceae/fisiologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...