RESUMO
Waste classification helps to maximize the recovery of resources, reduce environmental pollution and promote sustainable economic development. To improve the willingness of rural residents to classify waste, based on the background of a rural acquaintance society, this thesis constructed a theoretical model of waste classification cognition, willingness to classify, economic incentives, face consciousness and vernacular identity affecting the willingness of rural residents to classify waste, and put forward seven research hypotheses. With the help of 530 valid surveys of rural residents in Jiangsu Province, the seven hypotheses were verified using structural equation modeling, and the mediating effect of rural residents' willingness to classify waste was examined using the bootstrap method. The results showed that waste classification awareness, knowledge of waste classification, economic incentives, face-saving concepts and local identity all affected rural residents' willingness to classify waste, and all were significant at a 0.01 level. They also showed that face-saving concepts and local identity played partial mediating roles. Strategies to improve rural residents' willingness to classify waste have been proposed, such as multiple incentives to enhance subjective awareness, play the role of face culture in rural areas, strengthen the construction of public culture in rural areas, and improve rural residents' local identity.
Assuntos
População Rural , Humanos , China , Feminino , Masculino , Adulto , Inquéritos e Questionários , Pessoa de Meia-IdadeRESUMO
OBJECTIVE: To observe the changes of rapidly activated delayed rectifier potassium channel (IKr) and slowly activated delayed rectifier potassium channel (IKs) in cardiac hypertrophy and to evaluate the effects of IKr and IKs blocker on the incidence of ventricular arrhythmias in guinea pigs with left ventricular hypertrophy (LVH).â© Methods: Guinea pigs were divided into a sham operation group and a left ventricular hypertrophy (LVH) group. LVH model was prepared. Whole cell patch-clamp technique was used to record IKr and IKs tail currents in a guinea pig model with LVH. The changes of QTc and the incidence rate of ventricular arrhythmias in LVH guinea pigs were observed by using the IKr and IKs blockers.â© Results: Compared with cardiac cells in the control group, the interventricular septal thickness at end systole (IVSs), left ventricular posterior wall thickness at end systole (LVPWs), QTc interval and cell capacitance in guinea pigs with LVH were significantly increased (P<0.05); while IKs densities were significantly reduced [+60 mV: (0.36±0.03) pA/pF vs (0.58±0.05) pA/pF, P<0.01]. However, LVH exerted no significant effect on IKr densities. IKr blocker markedly prolonged the QTc interval (P<0.01) and increased the incidence of ventricular arrhythmias in guinea pigs with LVH compared with the control guinea pigs. In contrast, IKs blocker produced modest increase in QTc interval in guinea pigs of control group with no increase in LVH animals. IKs blocker did not induce ventricular arrhythmias incidence in either control or LVH animals.â© Conclusion: The cardiac hypertrophy-induced arrhythmogenesis is due to the down-regulation of IKs.
Assuntos
Arritmias Cardíacas/metabolismo , Regulação para Baixo , Hipertrofia Ventricular Esquerda/metabolismo , Canais de Potássio/metabolismo , Animais , Arritmias Cardíacas/tratamento farmacológico , Arritmias Cardíacas/etiologia , Modelos Animais de Doenças , Cobaias , Ventrículos do Coração/metabolismo , Hipertrofia Ventricular Esquerda/complicações , Técnicas de Patch-Clamp , Bloqueadores dos Canais de Potássio/farmacologia , Canais de Potássio Corretores do Fluxo de Internalização/antagonistas & inibidores , Canais de Potássio Corretores do Fluxo de Internalização/metabolismoRESUMO
Previous studies have found decreased functional capacity of the sodium pump (Na+-K+-ATPase) alpha and beta subunits and recovery of Na+-K+-ATPase activity significantly decreased myocyte apoptosis in myocardial ischemia-reperfusion (I/R) injury. However, the potential role of the Na+-K+-ATPase α-2 subunit (ATP1A2) in cardiomyocyte anoxia-reoxygenation (A/R) injury has not been elucidated. Rat myocardial cells were subjected to siRNA transfection followed by A/R injury. Apoptosis and expression of endoplasmic reticulum (ER) stress proteins CHOP, GRP78, and caspase-12 were detected in 4 groups of cells: ATP1A2 siRNA + A/R, control siRNA + A/R, control, and A/R injury model. We found that apoptosis was significantly elevated in the ATP1A2 siRNA + A/R group as compared with control siRNA + A/R, control, and A/R injury model groups (p < 0.05, p < 0.01, and p < 0.05). Furthermore, expression of CHOP, GRP78, and caspase-12 were significantly elevated in the ATP1A2 siRNA + A/R group as compared with control siRNA + A/R, control, and A/R injury model groups (p < 0.05, p < 0.01, and p < 0.05). Our findings suggest that cardiomyocyte ATP1A2 is a target of A/R injury, and its cardioprotective function may be mediated via inhibiting the ER-stress-related apoptosis.