Effects of chemical oxygen demand and chloramphenicol on attached microalgae growth: Physicochemical properties and microscopic mass transfer in biofilm.

During the wastewater treatment and resource recovery process by attached microalgae, the chemical oxygen demand (COD) can cause biotic contamination in algal culture systems, which can be mitigated by adding an appropriate dosage of antibiotics. The transport of COD and additive antibiotic (chloramphenicol, CAP) in algal biofilms and their influence on algal physiology were studied. The results showed that COD (60 mg/L) affected key metabolic pathways, such as photosystem II and oxidative phosphorylation, improved biofilm autotrophic and heterotrophic metabolic intensities, increased nutrient demand, and promoted biomass accumulation by 55.9 %, which was the most suitable COD concentration for attached microalgae. CAP (5-10 mg/L) effectively stimulated photosynthetic pigment accumulation and nutrient utilization in pelagic microalgal cells. In conclusion, controlling the COD concentration (approximately 60 mg/L) in the medium and adding the appropriate CAP concentration (5-10 mg/L) are conducive to improving attached microalgal biomass production and resource recovery potential from wastewater.

Long-term toxicity assessment of antibiotics against Vibrio fischeri: Test method optimization and mixture toxicity prediction.

The current luminescent bacteria test for acute toxicity with short contact time was invalid for antibiotics, and the non-uniformed contact times reported in the literature for long-term toxicity assessment led to incomparable results. Herein, a representative long-term toxicity assessment method was established which unified the contact time of antibiotics and Vibrio fischeri within the bioluminescence increasing period (i.e. 10-100% maximum luminescence) of control samples. The effects of excitation and detoxification of antibiotics such as ß-lactams were discovered. Half maximal inhibitory concentration (IC50) of toxic antibiotics (0.00069-0.061 mmol/L) obtained by this method was 2-3 orders of magnitude lower than acute test, quantifying the underestimated toxicity. As antibiotics exist in natural water as mixtures, an equivalent concentration addition (ECA) model was built to predict mixture toxicity based on physical mechanism rather than mathematical method, which showed great fitting results (R2 = 0.94). Furthermore, interaction among antibiotics was investigated. Antibiotics acting during bacterial breeding period had strong synergistic inhibition (IC50 relative deviation from 0.1 to 0.6) such as macrolides and quinolones. Some antibiotics produced increasing synergistic inhibition during concentration accumulation, such as macrolides. The discharge of antibiotics with severe long-term toxicity and strong synergistic inhibition effect should be seriously restricted.

Quantified trend of photosynthetic rate along the depth of microalgae biofilm.

Compared with suspended microalgae cultivation, attached microalgae cultivation for wastewater treatment has advantages of low biomass recovery costs and high robustness. As a heterogeneous system, the variation of photosynthetic capacity along biofilm depth lacks quantitative conclusions. The distribution curve of oxygen concentration along the depth of attached microalgae biofilm (f(x)) was detected by dissolved oxygen (DO) microelectrode, and a quantified model was built based on mass conservation and Fick's law. It revealed that the net photosynthetic rate at a certain depth (x) in the biofilm showed a linear relationship with the second derivatives of the distribution curve of oxygen concentration (f″(x)). In addition, the declining trend of photosynthetic rate along attached microalgae biofilm was relatively slow compared with the suspended system. The photosynthetic rate at 150-200 µm depth of algae biofilm was only 3.60 %-17.86 % of that at the surface layer. Moreover, the light saturation points of the attached microalgae got lower along the depth of biofilm. Compared to 400 lx light intensity, the net photosynthetic rate of microalgae biofilm at the depths of 100-150 µm and 150-200 µm increased by 389 % and 956 % under 5000 lx, respectively, showing the high photosynthesis potential with increasing light.

Screening of microalgae species and evaluation of algal-lipid stimulation strategies for biodiesel production.

Microalgae is considered an alternative source for biodiesel production producing renewable, sustainable and carbon-neutral energy. Microalgae property changes among species, which determines the efficiency of biodiesel production. Besides the lipid content evaluation, multi-principles (including high lipid productivity, high biomass yield, pollution resistance and desired fatty acid, etc.) for superior oil-producing species screening was proposed in this review and three microalgae species (Chlorella vulgaris, Scenedesmus obliquus and Mychonastes afer) with high bio-lipid producing prospect were screened out based on big data digging and analysis. The multilateral strategies for algal-lipid stimulating were also compared, among which, nutrient restriction, temperature control, heterotrophy and chemicals addition showed high potential in enhancing lipid accumulation; while electromagnetic field showed little effect. Interestingly, it was found that the lipid accumulation was more sensitive to nitrogen (N)-limitation other than phosphorus (P). Nutrient restriction, salinity stress etc. enhanced lipid accumulation by creating a stressed environment. Hence, optimum conditions (e.g. N:15-35 mg/L and P:4-16 mg/L) should be set to balance the lipid accumulation and biomass growth, and further guarantee the algal-lipid productivity. Otherwise, two-step cultivation could be applied during all the stressed stimulation. Different from lab study, effectiveness, operability and economy should be all considered for stimulation strategy selection. Nutrient restriction, temperature control and heterotrophy were highly feasible after the multidimensional evaluation.

Mechanism and performance of algal pond assisted constructed wetlands for wastewater polishing and nutrient recovery.

The limitation of oxygen and carbon source restricted the TN removal in constructed wetland (CW). Algal pond (AP) could produce oxygen and fix CO2 to improve C/N ratio in water. Therefore, an AP-CW system was established under laboratory conditions to deeply explore the effect of nutrient load distribution and microalgae addition in CWs on pollutant removal. This study showed that AP-CW could remove 49.7% TN and 90.0% TP with no carbon addition in CWs. The significant removal of NH4-N by AP advanced the location of denitrification in CWs. To enhance TN removal, different dosage of microalgae were intermittently added at 20 and 10 cm respectively below the inlet of the vertical flow CW1 and CW2, where the rest NH4-N has been almost oxidized into nitrate. The addition of microalgae influenced the microflora and effluent quality. Microalgae dosage in denitrification area significantly increased the absolute abundance of Σnir. The best TN removal of AP-CW could reach 91.3% when 8 g (dry weight) microalgae was added. However, unlike previous knowledge, microalgae as an organic carbon source would also release N and P during decomposition, leading to increased nutrients in the effluent. The optimal dosage of microalgae was 1 g/5 d in this study. The position and amount of microalgae addition in CWs should be adjusted based on water property and element flow to achieve the best pollutant removal and biomass harvest.

Synchronous synthesis of Cu2O/Cu/rGO@carbon nanomaterials photocatalysts via the sodium alginate hydrogel template method for visible light photocatalytic degradation.

A series of Cu2O/Cu/rGO@carbon nanomaterial (Cu2O/Cu/rGO@CN) heterogeneous photocatalysts were successfully synthesized synchronously via a novel sodium alginate hydrogel method. Cu2O nanoparticles (~50nm) were synthesized by calcination under the protection of a nitrogen atmosphere. Cu nanoparticles (~6nm) inevitably appeared on the surface of Cu2O, thereby forming a Cu2O/Cu heterostructure which is known as a Schottky junction. Graphene oxide (GO) nanosheets were synchronously reduced in situ by sodium alginate during the synthesis process and eventually acted as a 3-D structure with the assistance of the hydrogel skeleton. Because of the 3-D rGO modification, both the adsorption capacity and the photocatalytic activity of Cu2O/Cu/rGO@CN were significantly improved. The rate of p-nitrochlorobenzene (p-NCB) degradation catalyzed by Cu2O/Cu/rGO@CN was ~1.97×10-2min-1, which was much higher than that of the degradation catalyzed by Cu2O/Cu@CN (~0.239×10-2min-1). This result could be attributed to the two-stage Cu2O/Cu/rGO heterostructure, which facilitated efficient electron-hole separation. This method has the advantages of nontoxic raw materials, facile synthesis and reduced auxiliary usage, providing a new technique for designing heterogeneous photocatalysts.