[Gynecological acute abdomen in patients age under 18: an analysis of 237 cases].

Objective: To investigate the constituent ratio, clinical manifestation, diagnosis and treatment of gynecologic acute abdomen in patients age under 18. Methods: A retrospective study was conducted on 237 patients under 18 years old who had been admitted in Rizhao People's Hospital from June 2013 to November 2016. The patients were divided into two groups: groupⅠ (under ten years old, 49 cases) and group Ⅱ (ten to eighteen years old, 188 cases) . The first visit departments, constituent ratio, clinical manifestations and treatment methods of the two groups were summarized and analyzed. Results: The proportion of first visit department was gynecology department in the group Ⅰwas lower than that in group Ⅱ [18.4% (9/49) versus 69.1% (130/188) , P<0.01]. The proportion of diseases in the two groups was different; the proportion of ovarian tumors (59.2%, 29/49) , genital malformations (10.2%, 5/49) and ovarian torsions (10.2%, 5/49) in group Ⅰ were significantly higher than those in group Ⅱ, while the pregnancy related diseases in group Ⅱ (37.2%, 70/188) was significantly higher than that in group Ⅰ (P<0.01) . Conclusions: Clinicians should pay enough attention to the acute abdomen caused by gynecological factors in childhood and adolescence. The differential diagnosis of acute abdomen should pay attention to. In the choice of treatment methods, we should give full consideration to the special age, and try to maintain their normal growth and physiological function.

[Effect of workplace bullying on posttraumatic stress disorder in nursing staff].

Objective: To investigate the relationship between workplace bullying and posttraumatic stress disorder (PTSD) in nursing staff, and to analyze the role of psychological capital between workplace bullying and PTSD. Methods: From December 2014 to June 2015, convenience sampling was used to collect 496 nurses from 5 grade A tertiary hospitals in a province of China. Their workplace bullying, psychological capital, and PTSD status were assessed using the Negative Acts Questionnaire, Psychological Capital Questionnaire, and Posttraumatic Stress Disorder Self-Rating Scale, respectively. The correlation between variables was analyzed using a structural equation model. Results: Among these nurses, the scores of negative acts, psychological capital, and PTSD were 37.15±12.83, 78.81±16.54, and 34.56±12.52, respectively. The score on each dimension of negative acts was positively correlated with that on each dimension of PTSD (P<0.01) ; the score on each dimension of psychological capital was negatively correlated with that on each dimension of PTSD and negative acts (P<0.01). Negative acts had a positive predictive effect on PTSD (ß=0.539, P<0.01) , which was reduced after inclusion of psychological capital (ß=0.513, P<0.01). The path coefficient was 0.62 for the effect of negative acts on PTSD, -0.18 for the effect of negative acts on psychological capital, and -0.11 for the effect of psychological capital on PTSD (P<0.05) . Conclusion: Workplace bullying is a predictive factor for PTSD, and psychological capital plays a mediating role between workplace bullying and PTSD. The manager should reduce workplace bullying to improve the psychological capital in nursing staff and to prevent and reduce PTSD.

ClC-2 contributes to tonic inhibition mediated by α5 subunit-containing GABA(A) receptor in experimental temporal lobe epilepsy.

Temporal lobe epilepsy (TLE), characterized by spontaneous recurrent seizures, learning and memory impairments is associated with neurodegeneration, abnormal reorganization of the circuitry and loss of functional inhibition in hippocampus. In adult hippocampus, the GABAergic cells mediate the major inhibitory function of the principal neurons, promoting the Cl(-) entry through the GABA(A) receptor, whether through phasic (synaptic) or tonic (extrasynaptic) conductance. Aside from classical synaptic component, tonic GABAergic inhibition mediated by extrasynaptic GABA(A) receptor received increasing attention over the past years. There is growing evidence that tonic inhibition plays an important role in epilepsy, memory and cognition. Since GABA(A) receptor-mediated inhibition depends on the maintenance of intracellular Cl(-) concentration at low levels in mature neurons, a shift in E(cl) is likely to participate in the generation and not merely a consequence of TLE. As we know, chloride channel-2 (ClC-2) is a member of the supergene family of voltage-gated chloride channels, it constitutes part of the background conductance and is involved in chloride extrusion. Here we show that ClC-2 were upregulated functionally in CA1 pyramidal cells in pilocarpine-treated rats, and that an observed increase in ClC-2 currents in CA1 pyramidal cells was reversed by L655,708, a specific antagonist of α5 subunit-containing GABA(A) receptor.

Partial silencing of the NEC1 gene results in early opening of anthers in Petunia hybrida.

The NEC1 gene, previously isolated from Petunia hybrida, is expressed at high levels in nectaries, and in a very localized fashion in stamens, particularly in the anther stomium cells and the upper part of the filament. To elucidate the function of the NEC1 gene, co-suppression was employed for down-regulation of NEC1 expression, and transposon insertion mutagenesis was used to knock out the NEC1 function. Among the transgenic plants and plants carrying dTph1 inserted in the NEC1 gene, an "early open anther" phenotype was observed. In this mutant phenotype, the anthers already open in young flower buds (1.8 cm) that still contain immature pollen, resulting in poor pollen quality and impaired pollen release. The results obtained indicate that NEC1 might be involved in the development of stomium cells, which are ruptured during the normal process of anther dehiscence to release mature pollen. Southern analysis revealed the presence of a highly homologous NEC1-like gene, named NEC2, in the P. hybrida genome. The presence of NEC2 was confirmed by segregation analysis and sequencing of genomic clones. The implications of these results and possible reasons why no visually obvious phenotype in nectaries could be produced by co-suppression or transposon insertion mutagenesis are discussed.

NEC1, a novel gene, highly expressed in nectary tissue of Petunia hybrida.

To study the molecular regulation of nectary development, we cloned NEC1, a gene predominantly expressed in the nectaries of Petunia hybrida, by using the differential display RT-PCR technique. The secondary structure of the putative NEC1 protein is reminiscent of a transmembrane protein, indicating that the protein is incorporated into the cell membrane or the cytoplast membrane. Immunolocalization revealed that NEC1 protein is present in the nectaries. Northern blot analyses showed that NEC1 is highly expressed in nectary tissue and weakly in the stamen. GUS expression driven by the NEC1 promoter revealed GUS activity in the outer nectary parenchyma cells, the upper part of the filament and the anther stomium. The same expression pattern was observed in Brassica napus. GUS expression was observed as blue spots on the surface of very young nectaries that do not secrete nectar and do accumulate starch. GUS expression was highest in open flowers in which active secretion of nectar and starch hydrolysis had taken place. Ectopic expression of NEC1 resulted in transgenic plants that displayed a phenotype with leaves having 3-4 times more phloem bundles in mid-veins than the wild-type Petunia. The possible role of NEC1 gene in sugar metabolism and nectar secretion is discussed.

Clinical measurement of antibodies against acetylcholine receptor (AchR), SOD and LPO in patients with myasthenia gravis (MG) before and after thymectomy.

The antibodies against acetylcholine receptor AchR and levels of SOD and LPO were measured in 11 patients with myasthenia gravis (MG), and the results were compared with normal controls and patients with diseases other than MG. The results showed that the antibodies against AchR were higher as compared with other groups before and after operation. The post-operative level of antibodies was obviously lower than the pre-operative value. An slight increase in SOD and significant decrease in mean value of LPO after surgery were noted. The possible mechanism was discussed.

Rupture of thoracic aortic aneurysm into the lung with formation of pseudoaneurysm: diagnosis and treatment.

Three patients with rupture of thoracic aortic aneurysm into the lung with formation of pseudoaneurysm were admitted to our hospital in 1991, of whom, 2 had ascending aortic aneurysms which ruptured into the right upper pulmonary lobe, and another one had descending aortic aneurysm rupturing into the left upper lobe. All 3 cases were misdiagnosed as having pulmonary carcinoma or mediastinal tumor by the other hospital as well as by us. Correct diagnosis was made by aortography or at exploratory thoracotomy. Our preliminary experiences in the diagnosis and treatment of the disease are presented in this paper.

Surgical treatment of double-chamber right ventricle and its associated anomalies.

A report on the results of surgical treatment of 5 cases of DCRV and its associated anomaly was presented in this paper. No definite diagnosis of DCRV was made prior to operation. It was found that 2 cases had DCRV as an isolated anomaly, another 2 had the disease associated with VSD, and the remaining 1 had DCRV with right ventricle to left atrium cannulation. Muscular membrane septum type was found in 3 cases, and muscular bundle type in 2.4 cases diagnosed as having DCRV or DCRV with other heart anomalies during operation were treated properly and discharged fully recovered. 1 case died of acute heart insufficiency. Pathologic anatomy and physiology, associated anomalies of DCRV, and some experience with the operative approach were discussed in this article.