Expression of matrix metalloproteases-2 and -9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatment

Twenty horses were used in the experiment, for composed control group, (Cg) instrumented group, (Ig;without intestinal obstruction), treated group (Tg;submitted to intestinal obstruction and hydrocortisone treatment) and non-treated group (Ntg;submitted to intestinal obstruction without treatment). Immunohistochemistry and zymography techniques were used for researches on MMPs 2 and 9 in horse hoof laminae. There was an increase in the expression of MMP-2 in animals of Tg and Ntg. MMP-9 increased on animals from groups Ntg and Ig, however there was no rise of this MMP on the Tg when compared to the other groups in the immunohistochemistry analysis. Based on the results, it was observed that the intestinal injury caused by enterotomy and intestinal obstruction raise the quantities of MMPs in the hoof laminae.

Vinte cavalos foram usados no experimento: para compor o grupo controle (Cg), grupo instrumentado, Ig (sem obstrução intestinal), grupo tratado, Tg (submetidos à obstrução intestinal e tratamento com hidrocortisona) e grupo não tratado, Ntg (submetidos à obstrução intestinal, sem tratamento. Técnicas de zimografia e imunoistoquímica foram utilizadas para pesquisa de MMP-2 e MMP-9 no tecido laminar do casco dos equinos. Houve um aumento na expressão de MMP-2 nos animais dos grupos Tg e Ntg. A MMP-9 aumentou nos animais dos grupos Ig e Ntg. Houve aumento desta MMP no Tg quando comparado aos demais grupos na análise por zimografia. Observou-se que a injúria intestinal, causada pela enterotomia e obstrução intestinal, eleva a quantidade de MMPs no tecido laminar do casco.

Expression of matrix metalloproteases-2 and -9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatment

Twenty horses were used in the experiment, for composed control group, (Cg) instrumented group, (Ig;without intestinal obstruction), treated group (Tg;submitted to intestinal obstruction and hydrocortisone treatment) and non-treated group (Ntg;submitted to intestinal obstruction without treatment). Immunohistochemistry and zymography techniques were used for researches on MMPs 2 and 9 in horse hoof laminae. There was an increase in the expression of MMP-2 in animals of Tg and Ntg. MMP-9 increased on animals from groups Ntg and Ig, however there was no rise of this MMP on the Tg when compared to the other groups in the immunohistochemistry analysis. Based on the results, it was observed that the intestinal injury caused by enterotomy and intestinal obstruction raise the quantities of MMPs in the hoof laminae.

Vinte cavalos foram usados no experimento: para compor o grupo controle (Cg), grupo instrumentado, Ig (sem obstrução intestinal), grupo tratado, Tg (submetidos à obstrução intestinal e tratamento com hidrocortisona) e grupo não tratado, Ntg (submetidos à obstrução intestinal, sem tratamento. Técnicas de zimografia e imunoistoquímica foram utilizadas para pesquisa de MMP-2 e MMP-9 no tecido laminar do casco dos equinos. Houve um aumento na expressão de MMP-2 nos animais dos grupos Tg e Ntg. A MMP-9 aumentou nos animais dos grupos Ig e Ntg. Houve aumento desta MMP no Tg quando comparado aos demais grupos na análise por zimografia. Observou-se que a injúria intestinal, causada pela enterotomia e obstrução intestinal, eleva a quantidade de MMPs no tecido laminar do casco.

Topical treatment using amphotericin b and dmso for an atypically located equine cutaneous Pythiosis / Pitiose cutânea equina de localização atípica tratada topicamente com solução de anfotericina B e DMSO

Background: Cutaneous lesions by Pythium insidiosum infection are commonly observed in horses, especially in those living at fl ooded environments. Equine pythiosis is characterized by the development of tumoral masses that are frequently located at distal limbs, ventral abdômen, thorax, breast and face. The lesions are usually granulomatous, serosanguineous and ulcerated, most often destroyed by self-mutilation due to the intense pruritus. The proposed treatment includes surgical excision followed by antifungal drugs administration, which can be done systemically or topically. Amphotericin B and dimethyl sulfoxide (DMSO) in association has been successfully used for cutaneous pythiosis topical treatment due to the DMSO property to carry any substance through plasmatic membranes.Case: The present report concerns a 12-year-old mixed breed gelding presenting with self-mutilation of a tumoral mass located at the left fl ank. The owners reported that the horse had initially presented a small wound that had evolved to a 20-cm in diameter mass in 4 weeks. Tissue samples were collected, processed and stained by the Gomoris methenamine silver (GMS) method. The histopathological analysis revealed Pythium insidiosum hyphae in a granulomatous tissue, especially located at peripheral region, where kunkers were present. Surgical excision of the mass followed by cauterization was indicated

Background: Cutaneous lesions by Pythium insidiosum infection are commonly observed in horses, especially in those living at fl ooded environments. Equine pythiosis is characterized by the development of tumoral masses that are frequently located at distal limbs, ventral abdômen, thorax, breast and face. The lesions are usually granulomatous, serosanguineous and ulcerated, most often destroyed by self-mutilation due to the intense pruritus. The proposed treatment includes surgical excision followed by antifungal drugs administration, which can be done systemically or topically. Amphotericin B and dimethyl sulfoxide (DMSO) in association has been successfully used for cutaneous pythiosis topical treatment due to the DMSO property to carry any substance through plasmatic membranes.Case: The present report concerns a 12-year-old mixed breed gelding presenting with self-mutilation of a tumoral mass located at the left fl ank. The owners reported that the horse had initially presented a small wound that had evolved to a 20-cm in diameter mass in 4 weeks. Tissue samples were collected, processed and stained by the Gomoris methenamine silver (GMS) method. The histopathological analysis revealed Pythium insidiosum hyphae in a granulomatous tissue, especially located at peripheral region, where kunkers were present. Surgical excision of the mass followed by cauterization was indicated

Topical treatment using amphotericin b and dmso for an atypically located equine cutaneous Pythiosis / Pitiose cutânea equina de localização atípica tratada topicamente com solução de anfotericina B e DMSO

Background: Cutaneous lesions by Pythium insidiosum infection are commonly observed in horses, especially in those living at fl ooded environments. Equine pythiosis is characterized by the development of tumoral masses that are frequently located at distal limbs, ventral abdômen, thorax, breast and face. The lesions are usually granulomatous, serosanguineous and ulcerated, most often destroyed by self-mutilation due to the intense pruritus. The proposed treatment includes surgical excision followed by antifungal drugs administration, which can be done systemically or topically. Amphotericin B and dimethyl sulfoxide (DMSO) in association has been successfully used for cutaneous pythiosis topical treatment due to the DMSO property to carry any substance through plasmatic membranes.Case: The present report concerns a 12-year-old mixed breed gelding presenting with self-mutilation of a tumoral mass located at the left fl ank. The owners reported that the horse had initially presented a small wound that had evolved to a 20-cm in diameter mass in 4 weeks. Tissue samples were collected, processed and stained by the Gomoris methenamine silver (GMS) method. The histopathological analysis revealed Pythium insidiosum hyphae in a granulomatous tissue, especially located at peripheral region, where kunkers were present. Surgical excision of the mass followed by cauterization was indicated

Background: Cutaneous lesions by Pythium insidiosum infection are commonly observed in horses, especially in those living at fl ooded environments. Equine pythiosis is characterized by the development of tumoral masses that are frequently located at distal limbs, ventral abdômen, thorax, breast and face. The lesions are usually granulomatous, serosanguineous and ulcerated, most often destroyed by self-mutilation due to the intense pruritus. The proposed treatment includes surgical excision followed by antifungal drugs administration, which can be done systemically or topically. Amphotericin B and dimethyl sulfoxide (DMSO) in association has been successfully used for cutaneous pythiosis topical treatment due to the DMSO property to carry any substance through plasmatic membranes.Case: The present report concerns a 12-year-old mixed breed gelding presenting with self-mutilation of a tumoral mass located at the left fl ank. The owners reported that the horse had initially presented a small wound that had evolved to a 20-cm in diameter mass in 4 weeks. Tissue samples were collected, processed and stained by the Gomoris methenamine silver (GMS) method. The histopathological analysis revealed Pythium insidiosum hyphae in a granulomatous tissue, especially located at peripheral region, where kunkers were present. Surgical excision of the mass followed by cauterization was indicated

Anestesia por isofluorano em eqüinos submetidos à infusão contínua de medetomidina ou xilazina

Eight horses under inhalant general anesthesia with isoflurane (1MAC) and continuous infusion of xylazine (0.35mg kg-1h-1) or medetomidine (3.5µg kg-1h-1) were evaluated for heart rate and rhythm, respiratory rate, arterial blood pressure, arterial blood gas analysis and temperature immediately before the beginning of the continuous infusion (T0) and in intervals of 10 minutes after the beginning of the continuous infusion (T10 to T60). Heart rate and temperature decreased and mean arterial pressure increased. PaCO2 (in GM) increased and GM showed a higher paO2 than GX. We conclude that equipotent doses of continuous infusion of medetomidine and xylazine during inhalant general anesthesia with isoflurane in horses promote slight and equivalent cardiocirculatory, respiratory, thermic and arterial blood gases changes.

Avaliaram-se oito eqüinos sob anestesia geral inalatória com isofluorano (1CAM) e infusão contínua de xilazina (0,35mg kg-1h-1) ou medetomidina (3,5µg kg-1h-1), em relação à freqüência cardíaca, ritmo cardíaco, freqüência respiratória, pressão arterial, hemogasometria arterial e temperatura, nos tempos T0 (imediatamente antes do início da infusão contínua) e T10 ao T60 (intervalos de 10 minutos, após início da infusão contínua). Houve redução da freqüência cardíaca e da temperatura e elevação da pressão arterial média. A paCO2 (no GM) elevou-se e a paO2 mostrou-se maior no GM que no GX. Conclui-se que a infusão contínua de doses equipotentes de xilazina e medetomidina, durante anestesia geral inalatória, com isofluorano, em eqüinos, promove alterações cardiocirculatórias, respiratórias, térmicas e hemogasométricas discretas e equivalentes.

Anestesia por isofluorano em eqüinos submetidos à infusão contínua de medetomidina ou xilazina

Eight horses under inhalant general anesthesia with isoflurane (1MAC) and continuous infusion of xylazine (0.35mg kg-1h-1) or medetomidine (3.5µg kg-1h-1) were evaluated for heart rate and rhythm, respiratory rate, arterial blood pressure, arterial blood gas analysis and temperature immediately before the beginning of the continuous infusion (T0) and in intervals of 10 minutes after the beginning of the continuous infusion (T10 to T60). Heart rate and temperature decreased and mean arterial pressure increased. PaCO2 (in GM) increased and GM showed a higher paO2 than GX. We conclude that equipotent doses of continuous infusion of medetomidine and xylazine during inhalant general anesthesia with isoflurane in horses promote slight and equivalent cardiocirculatory, respiratory, thermic and arterial blood gases changes.

Avaliaram-se oito eqüinos sob anestesia geral inalatória com isofluorano (1CAM) e infusão contínua de xilazina (0,35mg kg-1h-1) ou medetomidina (3,5µg kg-1h-1), em relação à freqüência cardíaca, ritmo cardíaco, freqüência respiratória, pressão arterial, hemogasometria arterial e temperatura, nos tempos T0 (imediatamente antes do início da infusão contínua) e T10 ao T60 (intervalos de 10 minutos, após início da infusão contínua). Houve redução da freqüência cardíaca e da temperatura e elevação da pressão arterial média. A paCO2 (no GM) elevou-se e a paO2 mostrou-se maior no GM que no GX. Conclui-se que a infusão contínua de doses equipotentes de xilazina e medetomidina, durante anestesia geral inalatória, com isofluorano, em eqüinos, promove alterações cardiocirculatórias, respiratórias, térmicas e hemogasométricas discretas e equivalentes.

Clinical complications in nelore oocyte doners caused by vaginal ultrasound guided follicular aspiration / Complicações clínicas em vacas nelore doadoras de oócitos decorrentes da aspiração folicular transvaginal guiada por ultra-som

In the last few years the vaginal ultrasound guided follicular aspiration or OPU (ovum pick up) in bovine oocyte doners has become a very difunded technique. It has been used in females with highly zootechnical valuable. The complications of this technique, despite exceptional, assume great importance and might be of the veterinarian and owners knowledge. The purpose of this study is to describe the possible complications of the follicular aspiration considering the Veterinarian Hospital "Governador Laudo Natel" of the Faculdade de Ciências Agrárias e Veterinárias UNESP-Jaboticabal 2003-2005 casuistic. In a total number of 16 animals was possible to observe peritonitis, absence of labor signals, offspring death and peritonitis, distocic delivery with premature fetus, genital lesions, presence of vaginal wall abnormal masses and ovarian adhesions.KEY WORDS: Clinical complications, follicular aspiratiorn, Nelore cow.

Nos últimos anos, a aspiração folicular transvaginal guiada pela ultra-sonografia ou OPU (ovum pick up), em fêmeas bovinas doadoras de oócitos, tem se tornado uma prática muito difundida e vem sendo utilizada na multiplicação de fêmeas de elevado valor zootécnico. As complicações dessa técnica, mesmo que de ocorrência excepcional, assumem grande importância e devem ser de conhecimento do veterinário e, também, do proprietário. O propósito deste trabalho é descrever as possíveis complicações da aspiração folicular, considerando a casuística do Hospital Veterinário Governador Laudo Natel, da Faculdade de Ciências Agrárias e Veterinárias da UNESP, Jaboticabal, durante os anos de 2003 a 2005. Em um total de dezesseis animais recebidos, verificaram-se a presença de peritonite, a ausência de sinais de parto, morte fetal, parto distócico com feto prematuro, lesões de órgãos genitais, aumento de volume na parede da vagina e aderência ovariana.  PALAVRAS-CHAVES: Aspiração folicular, complicações clínicas, cava Nelore.

CLINICAL AND PATHOLOGICAL ASPECTS OF OVINE HEPATIC PHOTOSENSITIZATION OUTBREAK BY Brachiaria decumbens (Gramineae) INGESTION AT MATO GROSSO STATE / ASPECTOS CLÍNICOS E PATOLÓGICOS DE UM SURTO DE FOTOSSENSIBILIZAÇÃO HEPATÓGENA EM OVINOS PELA INGESTÃO DE Brachiaria decumbens (Gramineae) NO MUNICÍPIO DE CUIABÁ, MATO GROSSO

Photosensitization occurs in everyplace domestic animals are farmed along the world. Several studies are uncertain about Pithomyces chartarum involvement and its relationships with Brachiaria decumbens pasture in liver disease. This article describes clinical and pathological aspects observed at photosensitisation outbreak at Cuiabá, Mato Grosso, Brazil. Samples of liver, kidney, spleen and lymph nodes were collected after eleven sheep deaths and had sent to microscopic examination. Gall bladder epithelial cells proliferation, presence or absence of bile stasis, hepatic degeneration, cellular necrosis focus and multinucleated cells focus were found. The results appoints to closed relationship between hepatic and renal lesions and Brachiaria decumbens ingestion by sheeps. KEY WORDS: Brachiaria spp, hepatic lesions, ovine.

Fotossensibilização ocorre na maioria das áreas de criação de animais domésticos do mundo. Vários estudos questionam o envolvimento do fungo Pithomyces chartarum e sua relação com a gramínea Brachiaria decumbens na patogenia de lesões hepáticas. O presente trabalho tem como objetivo descrever os aspectos clínicos e patológicos observados num surto de fotossensibilização hepatógena ocorrido no município de Cuiabá, Mato Grosso, vindo a óbito ovinos mantidos exclusivamente em pasto de B. decumbens. Coletaram-se fragmentos de vários órgãos, tais como fígado, baço, rins e linfonodos para o processamento de secções histológicas que serviram de base para o estudo das lesões. Observaram-se proliferação de células epiteliais de ductos biliares, presença de bilestase, tumefação de hepatócitos, focos de lise de células e de células multinucleadas. Nos rins foram identificados túbulos contorcidos com sinais de lesões degenerativas. Os achados indicam que essas alterações hepáticas e renais podem ser relacionadas com a ingestão de Brachiaria decumbens.PALAVRAS-CHAVES: Brachiaria spp, lesões hepáticas, ovinos.

Implante de tubo de silicone com e sem colágeno na regeneração de nervos em eqüinos

Surgical nerve repair in humans, dogs and laboratory animals is widely utilized for important clinical and experimental purposes. However, in horses there is scarce information. Neuroma and excessive conjunctive proliferation are reported. The aim of the present study was to add information about the repair process utilizing implant of silicone tubes with or without collagen in nerve regeneration of horses. Eight horses were allocated in two groups: GI-13 weeks and GII-26 weeks of observation. A complete section of the ulnar nerves (UNs) and of the lateral cutaneous branch of the 17th thoracic nerves (TNs), bilaterally, was followed by repair with silicone tubes alternately filled with (STC) or without collagen (ST), with a 5mm gap between stumps. Clinical evaluation was performed weekly, by cutaneous sensitivity testing of the region innervated by the respective operated nerves and physical examination of the thoracic limbs. No alterations were found in the locomotor apparatus. The first positive reactions to the cutaneous test occurred at the 9th week post-surgery. At the end of the observation period it was verified, grossly, that the nerves were involved by conjunctive tissue and the lumen of the tube was filled by a whitish tissue, in a cylindrical shape. The ends nerves were interconnected. Microscopically, myelinated axons and Schwann cells were present in the gap between the stumps. In the GII the number of myelinated fibers was greater. The distal nerve showed remyelination process. There was no presence of neuroma and the inflammatory process was limited to the perineural areas. It was concluded that the implant of silicone tubes results in regeneration of UNs and TNs of horses without formation of neuromas and that de addition of collagen promotes an increase in the number of myelinated fibers.

A reconstituição cirúrgica de nervos em humanos, em cães e em diversos animais de laboratório é bastante utilizada e tem indicações clínicas e experimentais importantes. No entanto, em eqüinos há poucas informações sobre esta prática. Há relatos sobre a excessiva proliferação de tecido conjuntivo e a formação de neuromas à neurorrafia experimental, mesmo quando se utilizam tubos de silicone para condução do crescimento axonal. O presente estudo teve o objetivo de acrescentar informações sobre o processo de reparação de nervos periféricos em eqüinos por meio de implante de tubo de silicone preenchido ou não com colágeno. Para tanto, foram utilizados oito eqüinos, alocados em dois grupos: GI-13 semanas e GII-26 semanas de observação. Foi realizada secção dos nervos ulnares (NUs) e dos ramos cutâneos laterais dos 17° nervos torácicos (NTs), bilateralmente, seguindo tubulização, realizada em cada animal, alternando-se tubos de silicone vazios (TS), em um dos antímeros, ou preenchidos com solução de colágeno (TSC), no contralateral, deixando-se um espaço de 5mm entre os cotos. Nenhuma alteração foi encontrada ao exame do aparelho locomotor e as primeiras reações positivas ao teste de sensibilidade cutânea nos NUs e NTs com TS e TSC foram observadas a partir da 9ª semana, em ambos os grupos. Ao final do período de observação, verificou-se, macroscopicamente, que os nervos encontravam-se envolvidos por tecido conjuntivo e o interior da câmara estava preenchido por tecido de coloração esbranquiçada, de forma cilíndrica, interligando os cotos proximal e distal. Microscopicamente, constatou-se a presença de axônios mielinizados interligando os cotos, as células de Schwann e o processo de remielinização do coto distal, principalmente no TSC. Em ambos os grupos, não houve formação de neuromas e o processo inflamatório limitou-se às áreas perineurais. Concluiu-se que o implante de tubo de silicone conduz à regeneração de NUs e NTs de eqüinos sem formação de neuromas e que a adição de colágeno promove aumento do número de fibras mielinizadas.

Implante de tubo de silicone com e sem colágeno na regeneração de nervos em eqüinos

Surgical nerve repair in humans, dogs and laboratory animals is widely utilized for important clinical and experimental purposes. However, in horses there is scarce information. Neuroma and excessive conjunctive proliferation are reported. The aim of the present study was to add information about the repair process utilizing implant of silicone tubes with or without collagen in nerve regeneration of horses. Eight horses were allocated in two groups: GI-13 weeks and GII-26 weeks of observation. A complete section of the ulnar nerves (UNs) and of the lateral cutaneous branch of the 17th thoracic nerves (TNs), bilaterally, was followed by repair with silicone tubes alternately filled with (STC) or without collagen (ST), with a 5mm gap between stumps. Clinical evaluation was performed weekly, by cutaneous sensitivity testing of the region innervated by the respective operated nerves and physical examination of the thoracic limbs. No alterations were found in the locomotor apparatus. The first positive reactions to the cutaneous test occurred at the 9th week post-surgery. At the end of the observation period it was verified, grossly, that the nerves were involved by conjunctive tissue and the lumen of the tube was filled by a whitish tissue, in a cylindrical shape. The ends nerves were interconnected. Microscopically, myelinated axons and Schwann cells were present in the gap between the stumps. In the GII the number of myelinated fibers was greater. The distal nerve showed remyelination process. There was no presence of neuroma and the inflammatory process was limited to the perineural areas. It was concluded that the implant of silicone tubes results in regeneration of UNs and TNs of horses without formation of neuromas and that de addition of collagen promotes an increase in the number of myelinated fibers.

A reconstituição cirúrgica de nervos em humanos, em cães e em diversos animais de laboratório é bastante utilizada e tem indicações clínicas e experimentais importantes. No entanto, em eqüinos há poucas informações sobre esta prática. Há relatos sobre a excessiva proliferação de tecido conjuntivo e a formação de neuromas à neurorrafia experimental, mesmo quando se utilizam tubos de silicone para condução do crescimento axonal. O presente estudo teve o objetivo de acrescentar informações sobre o processo de reparação de nervos periféricos em eqüinos por meio de implante de tubo de silicone preenchido ou não com colágeno. Para tanto, foram utilizados oito eqüinos, alocados em dois grupos: GI-13 semanas e GII-26 semanas de observação. Foi realizada secção dos nervos ulnares (NUs) e dos ramos cutâneos laterais dos 17° nervos torácicos (NTs), bilateralmente, seguindo tubulização, realizada em cada animal, alternando-se tubos de silicone vazios (TS), em um dos antímeros, ou preenchidos com solução de colágeno (TSC), no contralateral, deixando-se um espaço de 5mm entre os cotos. Nenhuma alteração foi encontrada ao exame do aparelho locomotor e as primeiras reações positivas ao teste de sensibilidade cutânea nos NUs e NTs com TS e TSC foram observadas a partir da 9ª semana, em ambos os grupos. Ao final do período de observação, verificou-se, macroscopicamente, que os nervos encontravam-se envolvidos por tecido conjuntivo e o interior da câmara estava preenchido por tecido de coloração esbranquiçada, de forma cilíndrica, interligando os cotos proximal e distal. Microscopicamente, constatou-se a presença de axônios mielinizados interligando os cotos, as células de Schwann e o processo de remielinização do coto distal, principalmente no TSC. Em ambos os grupos, não houve formação de neuromas e o processo inflamatório limitou-se às áreas perineurais. Concluiu-se que o implante de tubo de silicone conduz à regeneração de NUs e NTs de eqüinos sem formação de neuromas e que a adição de colágeno promove aumento do número de fibras mielinizadas.

CLINICAL AND PATHOLOGICAL ASPECTS OF OVINE HEPATIC PHOTOSENSITIZATION OUTBREAK BY Brachiaria decumbens (Gramineae) INGESTION AT MATO GROSSO STATE / ASPECTOS CLÍNICOS E PATOLÓGICOS DE UM SURTO DE FOTOSSENSIBILIZAÇÃO HEPATÓGENA EM OVINOS PELA INGESTÃO DE Brachiaria decumbens (Gramineae) NO MUNICÍPIO DE CUIABÁ, MATO GROSSO

Photosensitization occurs in everyplace domestic animals are farmed along the world. Several studies are uncertain about Pithomyces chartarum involvement and its relationships with Brachiaria decumbens pasture in liver disease. This article describes clinical and pathological aspects observed at photosensitisation outbreak at Cuiabá, Mato Grosso, Brazil. Samples of liver, kidney, spleen and lymph nodes were collected after eleven sheep deaths and had sent to microscopic examination. Gall bladder epithelial cells proliferation, presence or absence of bile stasis, hepatic degeneration, cellular necrosis focus and multinucleated cells focus were found. The results appoints to closed relationship between hepatic and renal lesions and Brachiaria decumbens ingestion by sheeps. KEY WORDS: Brachiaria spp, hepatic lesions, ovine.

Fotossensibilização ocorre na maioria das áreas de criação de animais domésticos do mundo. Vários estudos questionam o envolvimento do fungo Pithomyces chartarum e sua relação com a gramínea Brachiaria decumbens na patogenia de lesões hepáticas. O presente trabalho tem como objetivo descrever os aspectos clínicos e patológicos observados num surto de fotossensibilização hepatógena ocorrido no município de Cuiabá, Mato Grosso, vindo a óbito ovinos mantidos exclusivamente em pasto de B. decumbens. Coletaram-se fragmentos de vários órgãos, tais como fígado, baço, rins e linfonodos para o processamento de secções histológicas que serviram de base para o estudo das lesões. Observaram-se proliferação de células epiteliais de ductos biliares, presença de bilestase, tumefação de hepatócitos, focos de lise de células e de células multinucleadas. Nos rins foram identificados túbulos contorcidos com sinais de lesões degenerativas. Os achados indicam que essas alterações hepáticas e renais podem ser relacionadas com a ingestão de Brachiaria decumbens.PALAVRAS-CHAVES: Brachiaria spp, lesões hepáticas, ovinos.

CLINICAL COMPLICATIONS IN NELORE OOCYTE DONERS CAUSED BY VAGINAL ULTRASOUND GUIDED FOLLICULAR ASPIRATION / COMPLICAÇÕES CLÍNICAS EM VACAS NELORE DOADORAS DE OÓCITOS DECORRENTES DA ASPIRAÇÃO FOLICULAR TRANSVAGINAL GUIADA POR ULTRA-SOM

In the last few years the vaginal ultrasound guided follicular aspiration or OPU (ovum pick up) in bovine oocyte doners has become a very difunded technique. It has been used in females with highly zootechnical valuable. The complications of this technique, despite exceptional, assume great importance and might be of the veterinarian and owners knowledge. The purpose of this study is to describe the possible complications of the follicular aspiration considering the Veterinarian Hospital "Governador Laudo Natel" of the Faculdade de Ciências Agrárias e Veterinárias UNESP-Jaboticabal 2003-2005 casuistic. In a total number of 16 animals was possible to observe peritonitis, absence of labor signals, offspring death and peritonitis, distocic delivery with premature fetus, genital lesions, presence of vaginal wall abnormal masses and ovarian adhesions.KEY WORDS: Clinical complications, follicular aspiratiorn, Nelore cow.

Nos últimos anos, a aspiração folicular transvaginal guiada pela ultra-sonografia ou OPU (ovum pick up), em fêmeas bovinas doadoras de oócitos, tem se tornado uma prática muito difundida e vem sendo utilizada na multiplicação de fêmeas de elevado valor zootécnico. As complicações dessa técnica, mesmo que de ocorrência excepcional, assumem grande importância e devem ser de conhecimento do veterinário e, também, do proprietário. O propósito deste trabalho é descrever as possíveis complicações da aspiração folicular, considerando a casuística do Hospital Veterinário Governador Laudo Natel, da Faculdade de Ciências Agrárias e Veterinárias da UNESP, Jaboticabal, durante os anos de 2003 a 2005. Em um total de dezesseis animais recebidos, verificaram-se a presença de peritonite, a ausência de sinais de parto, morte fetal, parto distócico com feto prematuro, lesões de órgãos genitais, aumento de volume na parede da vagina e aderência ovariana.  PALAVRAS-CHAVES: Aspiração folicular, complicações clínicas, cava Nelore.