Utilization of tannery wastewater for biofuel production: New insights on microalgae growth and biomass production.

Microalgae cultivation on tannery wastewater (TWW) has been examined in some studies as a possible biological application to reduce contamination load and discharge effluents safely. However, Growth aspects, different tolerate strains and enriching the medium were not well investigated. In our study we applied Scenedesmus sp., Chlorella variabilis and Chlorella sorokiniana with different TWW concentrations. C. sorokiniana and C. variabilis cell density, chlorophyll, and sugar content grew substantially as compared to control. C. sorokiniana biomass and total lipids folded three and two times in 25% and 40% TWW, respectively as compared to control. Scenedesmus sp. showed longer lag phase and lower performance compared to the other two strains. Kelp waste extract (KWE) was added to balance the nutrients supply for C. sorokiniana, of which growth and effluents indicators were then greatly promoted in all concentrations. As the lag phase was shortened from 8 to 4 days in 60% concentration, subsequently, chlorophyll, carbohydrates, biomass and total lipids appreciated by 184%, 400%, 162% and 135%, respectively. Furthermore, the COD and ammonium removals improved by 51% and 45%, respectively. These outcomes emphasize the suitability of using TWW for microalgae cultivation with the suitable concentration while adding kelp waste extract for further enhancement.

Improvement on lipid production by Scenedesmus obliquus triggered by low dose exposure to nanoparticles.

Carbon nanotubes (CNTs), α-Fe2O3 nanoparticles (nano Fe2O3) and MgO nanoparticles (nano MgO) were evaluated for the effects on algae growth and lipid production. Nano Fe2O3 promoted cell growth in the range of 0-20 mg·L-1. CNTs, nano Fe2O3 and nano MgO inhibited cell growth of Scenedesmus obliquus at 10, 40 and 0.8 mg·L-1 respectively. Neutral lipid and total lipid content increased with the increasing concentration of all tested nanoparticles. The maximum lipid productivity of cultures exposed to CNTs, nano Fe2O3 and nano MgO was observed at 5 mg·L-1, 5 mg·L-1 and 40 mg·L-1, with the improvement by 8.9%, 39.6% and 18.5%. High dose exposure to nanoparticles limited increase in lipid productivity, possibly due to the repression on cell growth caused by nanoparticles-catalyzed reactive oxygen species (ROS) generation, finally leading to reduction in biomass and lipid production. Reduced accumulation of fatty acids of C18:3n3, C18:3n6 and C20:2 was observed in cells exposed to nanoparticles.

Kelp waste extracts combined with acetate enhances the biofuel characteristics of Chlorella sorokiniana.

To probe the effect of kelp waste extracts (KWE) combined with acetate on biochemical composition of Chlorella sorokiniana, the cultures were performed under independent/combined treatment of KWE and acetate. The results showed that high cell density and biomass were obtained by KWE combined with acetate treatments, whose biomass productivity increased by 79.69-102.57% and 20.04-35.32% compared with 3.0gL-1 acetate and KWE treatments respectively. The maximal neutral lipid per cell and lipid productivity were gained in KWE combined with 3.0gL-1 acetate treatment, which increased by 16.32% and 129.03% compared with 3.0gL-1 acetate, and 253.35% and 70.74% compared with KWE treatment. Meanwhile, C18:3n3 and C18:2n6c contents were reduced to 4.90% and 11.88%, whereas C16:0 and C18:1n9c were improved to 28.71% and 37.76%. Hence, supplementing appropriate acetate in KWE cultures is supposed to be a great potential method for large-scale cultivation of C. sorokiniana to generate biofuel.