Development, validation and field application of an RNA-based growth index in juvenile plaice Pleuronectes platessa.

A general mechanism relating RNA concentration and growth rate is derived from four physiological assumptions and developed into a growth index for juvenile plaice Pleuronectes platessa. The index describing instantaneous growth rates (G, day⁻¹) in the laboratory with the lowest Akaike information criterion with small-sample bias adjustment was a function of RNA concentration (R, g(RNA)g⁻¹(wet mass)), temperature (T, ° K), body mass (M, g) and DNA concentration (D, g(DNA)g⁻¹(wet mass)): G = ß0 + ß(R) R + ß(T)T + ß(T2)T² + ß(M)M + ß(D)D + ß(RT)RT. RNA concentration began to respond to changes in feeding conditions within 8 days, suggesting that the index reflects growth rate in the short-term. Furthermore, the index distinguished between rapid growth and negative growth of juvenile P. platessa measured directly in laboratory and field enclosures, respectively. An application of the RNA-based growth index at two beaches on the west coast of Scotland suggested that the growth of juvenile P. platessa varies considerably in space and time and is submaximum in late summer.

The effect of Prestige oil ingestion on the growth and chemical composition of turbot otoliths.

Juvenile turbot (Scophthalmus maximus) were kept in captivity and were fed a prepared food contaminated with five different concentrations of seawater-accommodated fuel oil from 2.4+/-0.35 to 48.2+/-2.2 mg g(-1) food, with a control group receiving uncontaminated food. The growth and survival of individually tagged fish (N=202) were measured after a six-week treatment period. The otolith growth rate was measured and otolith composition was determined before and after the treatments using LA-ICPMS. Fish and otolith growth were negatively affected by the fuel oil treatment, and the response decreased with the level of contamination. Otolith growth and element incorporation peaked at mid level exposures and decreased at the highest level. The otolith elemental composition reflected the presence of some elements in the Prestige fuel that may have been incorporated through the diet into the otolith.