Age related decreases in thermoregulation and cardiovascular function in horses.

REASON FOR PERFORMING STUDY: Older horses have an increased risk of hyperthermia due to impaired cardiovascular function. While many studies have investigated thermoregulation in horses during exercise, none have investigated the effects of ageing. OBJECTIVE: To test the hypothesis that there is a difference in thermoregulation during exercise and plasma volume (PV) in young and old horses. METHODS: Study 1: 6 young (Y, 7.7 ± 0.5 years) and 5 old (O, 26.0 ± 0.8 years) unfit Standardbred mares (507 ± 11 kg, mean ± s.e.) ran on a treadmill (6% grade, velocity calculated to generate a work rate of 1625 watts) until core temperature reached 40 °C. Core (CT), skin (ST), rectal temperature (RT) and heart rate (HR) were measured every min until 10 min post exertion. Packed cell volume (HCT), lactate (LA) and plasma protein (TP) were measured in blood samples collected before, at 40 °C and every 5 min until 10 min post exercise. Sweat loss was estimated using bodyweight. Study 2: Plasma volume was measured in 26 young (8.2 ± 0.7 years) and 8 old (26.6 ± 0.7 years) Standardbred mares (515 ± 12 kg) using Evans Blue dye. Pre-exercise blood (rBV) and red cell (rRCV) volumes were calculated using PV and HCT. Data analysis utilised repeated measures ANOVA and t tests and data are expressed as mean ± s.e. RESULTS: Old horses reached 40 °C faster (998 ± 113 vs. 1925 ± 259 s; P < 0.05) with a greater HR at 40 °C (184 ± 6 vs. 140 ± 5 beats/min; P < 0.05) and greater sweat losses (P < 0.05). Heart rate did not differ (P > 0.05) post exercise. Age did not alter (P > 0.05) CT, ST, RT, LA, HCT or TP. Plasma volume was greater in Y vs. O horses (P < 0.05, 28.5 ± 1.4 vs. 24.1 ± 1.6 l) as was rBV (41.3 ± 2.0 vs. 35.3 ± 2.3 l) and rRCV (13.3 ± 0.6 vs. 11.1 ± 0.8 l). CONCLUSION: Ageing compromises the ability to handle the combined demand of exercise and thermoregulation in part due to decreased absolute pre-exercise PV.

Neuropatia dolorosa isolada: uma variante da doença de Fabry / Isolated painful neuropathy: an atypical variant of Fabry disease

A doença de Anderson-Fabry é uma desordem de acúmulo lisossomial causada por deficiência da enzima alfa-galactosidase A (@-Gal A), relacionada com mutação recessiva no cromossomo X, resultando em acúmulo de glicolípides nas células endoteliais, nas células musculares lisas, nas células periteliais vasculares, e, variavelmente, nos neurônios e células ganglionares. O quadro clínico inicia-se na adolescência, com dores intensas e recorrentes nas extremidades, seguidas por angioceratomas, distrofia corneana, insuficiência renal e comprometimento cardíaco. Pacientes com dosagem de ?-Gal A extremamente baixa tendem a evoluir para óbito ao redor dos 40 anos de idade devido à insuficiência renal, infarto do miocárdio ou acidente vascular cerebral. Comprometimento isolado ou do coração ou do rim está relacionado com dosagem ao redor de 1 a 10% da @-Gal A. Descrevemos um paciente de 63 anos, masculino, apresentando, exclusivamente, acroparestesia dolorosa crônica e intensa, com dosagem não detectável de @-Gal A e comprometimento exclusivo de nervos periféricos, principalmente de fibras finas. Biópsia de pele mostrou ausência de nervos intraepidérmicos. A Doença de Fabry com neuropatia exclusiva é uma variante ainda não descrita.

Anderson-Fabry disease is a lysosomal storage disorder caused by a deficiency of the enzyme alpha-galactosidase (@-Gal A) related to recessive mutation carried on the X chromosome, resulting in glicolipid accumulation in the cytoplasm of endothelial cells, smooth muscle cells, and perithelial cells of blood vessels, and variably in neurons and ganglionic cells. Clinical onset of the disease occurs during adolescence with recurrent episodes of severe pain in the extremities, followed by angiokeratomas, cornea dystrophy, renal insufficiency and cardiac involvement. Patients that have extremely low @-Gal A activity are likely to evolve to death at about 40 years of age due to renal failure, heart attack or brain stroke. We present a 63 year-old man, showing exclusively chronic and severe painful acroparesthesia, with undetectable @-Gal A and exclusive involvement of peripheral nerves, mainly thin fibers. Skin biopsy showed no intraepidermal nerve fibers. Fabry disease manifested with exclusive peripheral neuropathy is a variant not yet described.

Low dose exogenous erythropoietin elicits an ergogenic effect in standardbred horses.

REASONS FOR PERFORMING STUDY: Recombinant human erythropoietin (rhuEPO) causes an increase in red blood cell production and aerobic capacity in other species; however, data are lacking on effects in the horse. HYPOTHESIS: This study tested the hypothesis that rhuEPO administration would alter red cell volume (RCV), aerobic capacity (VO2max) and indices of anaerobic power. METHODS: Eight healthy, unfit mares accustomed to the laboratory and experimental protocols were randomly assigned to either a control (CON, n = 4; 3 ml saline 3 times/week for 3 weeks) or EPO group (EPO, n = 4, 50 iu/kg bwt rhuEPO/3 ml saline 3 times/week for 3 weeks). Exercise tests (GXT) were performed on a treadmill (6% incline), 1 week before and 1 week after treatment. The GXT started at 4 m/sec, with a 1 m/sec increase every 60 sec until the horse reached fatigue. Oxygen uptake was measured via an open flow indirect calorimeter. Blood samples were collected before, during (each step) and 2 and 15 min post GXT to measure packed cell volume (PCV), haemoglobin concentration (Hb), blood lactate concentration (LA) and plasma protein concentration (TP). Plasma volume (PV) was measured using Evans Blue dye. Blood volume (BV) and RCV were calculated using PCV from the 8 m/sec step of the GXT. RESULTS: There were no alterations (P>0.05) in any parameters in CON horses. By week 3, EPO produced increases (P<0.05) in resting PCV (37 +/- 2 vs. 51 +/- 2) and Hb (37%). RCV (26%) and VO2max (19%) increased, but BV did not change (P>0.05) due to decreased PV (-11%, P<0.05). There was a significant increase in velocity at VO2max and LApeak for horses treated with rhuEPO and substantial decrease (P<0.05) in VO2 recovery time when the pretreatment GXT was compared to the post treatment GXT. No differences (P<0.05) were detected for TP, VLA4, run time or Vmax. CONCLUSIONS: Low dose rhuEPO administration increases RCV and aerobic capacity without altering anaerobic power. POTENTIAL RELEVANCE: This study demonstrates that rhuEPO enhances aerobic capacity and exercise performance, a question relevant to racing authorities.

Plasma aldosterone concentration and renal sodium excretion are altered during the first days of training.

The purpose of the present study was to determine whether the training-induced hypervolaemic response seen in the horse is associated with aldosterone-mediated renal mechanisms affecting sodium conservation during the first days of training. Five healthy, Standardbred mares (weight 450-500 kg, age 4-8 years) that were unfit, but accustomed to running on the treadmill, were used to test the hypothesis that repeated submaximal exercise would alter plasma aldosterone (ALDO) concentration and renal excretion of electrolytes in horses within the first 3 days of training. The experiment consisted of a 2 week housing equilibration period followed by a 1 week control period and a 3 day exercise training period (30 min/day at 60% VO2max). During control, ALDO and renal fluid and electrolyte losses were measured for 24 h on 3 separate days. Renal function (urine volume [UV], 24 h excretion of Na+, K+ and Cl- [UNA+ V, UK+ V, UCl- V], clearance of Na+ [CNa+], K+ [CK+] and Cl- [CCl-], creatinine [CCr], osmotic substances [Cosm], and solute-free water [FWC], and the fractional excretion of Na+, K+ and Cl-) and ALDO were measured for an additional 3 consecutive days during the training period. There were no differences (P>0.05) in any variable during the control period. Plasma volume increased (+18.7%, P<0.05) after 3 days of training. During training, there were no significant changes in plasma osmolality, electrolyte concentrations or CCr. Training caused decreases (P<0.05) in UV (-30%), UNA+ V (-73%), UK+ V, (-55%) and UCl- V (-70%). Training also caused decreases (P<0.05) in Cosm (-30%), through decreases in CNa+ (-60%), CK+ (-60%), and CCl- (-66%). Interestingly, FWC increased (+30%, P<0.05), whereas, there were significant decreases in the fractional excretion of Na+ (-59%), K+ (-48%) and Cl- (-60%). Training caused substantial elevations in both pre-exercise (967%, P<0.05) and postexercise (+3013%, P<0.05) plasma ALDO concentrations suggesting an increase in both basal levels and the responsiveness to acute exercise. Together, these observations suggest that mechanisms affecting tubular conservation of electrolytes contribute to the early response to training. However, it is also concluded that renal mechanisms appear to be only part of the mechanism for conserving sodium and water intake as well as training-induced changes in gastrointestinal mechanisms affecting electrolyte and water balance.

Fortalecimento ambiental municipal: necessidades e perspectivas / Environmental enforcement: needs and perspectives

Expöe sobre os aspectos legais do enfrentamento das questöes ambientais, situando o município dentro da necessidade de participaçäo que emergiu com o advento da Constituiçäo Federal de 1988, tornando-se ainda mais urgente a partir dos instrumentos legais da Resoluçäo 237/CONAMA/97 e Lei 9.605/98, que estabelecem novos parâmetros o licenciamento ambiental para o que se irá considerar infraçäo ambiental daqui para a frente

Myoelectric activity of the cecum and proximal loop of the ascending colon in cows after spontaneous cecal dilatation/dislocation.

OBJECTIVE: To analyze myoelectric activity of the cecum and proximal loop of the ascending colon (PLAC) in cows after spontaneous cecal dilatation/dislocation (CDD) and compare it with that in healthy cows after surgical evacuation of the cecum. ANIMALS: 12 cows with spontaneous CDD and 6 healthy cows (group C). Cows with spontaneous CDD were retrospectively assigned to 2 groups: delayed recovery from surgery or recurrence (group A; n = 3), and normal recovery (group B; n = 9). PROCEDURE: After surgical evacuation of the cecum, 8 pairs of bipolar, retrievable electrodes were implanted in the ileum, cecum, and PLAC. Cows were evaluated daily from postoperative day 1 to 7, using routine clinical methods and computer-based analysis of myoelectric activity of the cecum, and PLAC. Parameters of myoelectric activity included rate of spike bursts, duration of individual spike bursts, duration of overall spike burst activity per electrode, rate of propagated spike burst sequences, and ratio of orally propagated spike burst sequences. RESULTS: Rate of spike bursts, duration of cecocolic spike burst activity, and ratio of orally to aborally propagated spike burst sequences did not vary among groups during the 7-day recording period. However, cows with delayed recovery had a typical, uniform pattern of myoelectric activity of the cecum and PLAC at days 1 and 2 after surgery that consisted of repeated, propagated spike burst sequences, made up of spike bursts of significantly (P < 0.05) increased duration at postsurgical day 1 and substantially prolonged duration at postsurgical day 2, interrupted by periods of little or no activity. CONCLUSION: Delayed recovery and recurrence of CDD in cows after spontaneous CDD is not caused by hypomotility of the cecum and PLAC. CLINICAL RELEVANCE: Postoperative treatment, intended to reduce recurrence of CDD or delayed recovery after surgical evacuation of the cecum, should address propagation of digesta in the spiral colon.

[Effectiveness of local treatment using a PVP-iodine compound for retained placenta in cows]. / Untersuchungen zur Wirksamkeit der lokalen Behandlung mit einem PVP-Jodpräparat bei der Nachgeburtsverhaltung der Kuh.

106 cattle with retained placenta were treated either with Vetisept-bolus or Tetran-bolus (controls) after an attempt to remove fetal membranes. Uterine involution judged by rectal palpation on the 28th day was the same in both groups, but more treatments were necessary for cows of the experimental group. Cows treated with Vetisept came in heat nine days earlier than controls (p < 0.05). The pregnancy rate of the controls (91.6%) was better (p < 0.05) than in the experimental group (60.7%). No significant differences between the two groups were encountered for pregnancy index, total index, and service period. Of 82 cows after single calving 50 got pregnant again. In 18 of the remaining cattle another pregnancy was not attempted. The other 14 were bred but did not conceive. Treatment with tetracycline seems to be slightly superior and safer than the treatment with PVP-iodine. It is uncertain, whether the higher (p < 0.05) losses of animals in the experimental group than in the controls is due to the different treatment, for most of the cows had been slaughtered because of insufficient milk production. Therapy with iodine is a recommended alternative to the treatment with tracycline for certain indications.