RESUMO
OBJECTIVE: To study the chemical constituents in stem rind of Wikstroemia indica. METHOD: The constituents were isolated by column chromatographies with silica gel and ODS, and identified by NMR, MS spectra. RESULT: Six compounds, daphnoretin-7-O-beta-D-glucoside (1), quercitin (2), physcion (3), kaempferol 3-rutinoside (4), D-primev-ersyl genkwanine (5), and anabellamide (6) were isolated and identified. CONCLUSION: All of them were isolated from this plant for the first time.
Assuntos
Plantas Medicinais/química , Quercetina/análogos & derivados , Wikstroemia/química , Emodina/análogos & derivados , Emodina/química , Emodina/isolamento & purificação , Estrutura Molecular , Caules de Planta/química , Quercetina/química , Quercetina/isolamento & purificaçãoRESUMO
OBJECTIVE: To develop an HPLC method for determination of components in root of Saposhnikovia divaricata. METHOD: The separation of four components was achieved on a reversed-phase Alltima C18 column with MeOH-H2O gradient elution at a flow rate of 1.0 mL x min(-1). RESULT: Six samples were determined and there is no distinct difference between the contents determined by the new and old methods. CONCLUSION: The new method is more simple and convenient than the old method.
Assuntos
Apiaceae/química , Medicamentos de Ervas Chinesas/análise , Monossacarídeos/análise , Plantas Medicinais/química , Xantenos/análise , Cromatografia Líquida de Alta Pressão/métodos , Raízes de Plantas/químicaRESUMO
OBJECTIVE: To study the chemical constituents form stem bark of Wikstroemia indica. METHOD: The constituents were isolated by column chromatography on silica gel and ODS, and identified by spectroscopic methods. RESULT: Seven compounds, daphnoretin (I), tricin (II), daucosterol (III), beta-sitosterol (IV), umbelliferone (V), genkwanin (VI), and 6'-hydroxy, 7-O-7'-dicoumarin (VII), were isolated and identified. CONCLUSION: Compound VII was a new compound and compounds III, V were isolated from this plant for the first time.
Assuntos
Cumarínicos/química , Cumarínicos/isolamento & purificação , Plantas Medicinais/química , Wikstroemia/química , Estrutura Molecular , Caules de Planta/química , Sitosteroides/química , Sitosteroides/isolamento & purificação , Umbeliferonas/química , Umbeliferonas/isolamento & purificaçãoRESUMO
OBJECTIVE: To establish a quality standard of Peucedanum praeruptorum for Chinese Pharmacopoeia (2005). METHOD: The presence of (+/-)-praeruptorin A were identified and assayed by TLC and HPLC, respectively. RESULT: Linearity of marker was obtained over the range of 0.17696-0.88480 microg (r=0.9999). The average recovery rate was 99.72% (RSD=0.40%). CONCLUSION: TLC is specific. The method of quantity is accurate, reappearance, simple, rapid, and suitable for the quality control of P. praeruptorum.
