Improvement of the rapid response system at an acute rehabilitation hospital in New Mexico.

Aim: Enhance the Rapid Response System (RRS) in a free-standing acute rehabilitation hospital (ARH) by improving announcements, crash cart standardization and role assignments. Materials & methods: Pre-intervention (PreIQ) and post-intervention questionnaires (PostIQ), conducted in English and utilizing a Likert scale, were distributed in-person to clinical staff, yielding a 100% response rate. The questionnaire underwent no prior testing. The PreIQ were disseminated in February 2021, and PostIQ in December 2022. Results: PostIQ illustrated the improvement of audibility and improved the clarity of roles. The training positively impacted the RRS in the ARH. Conclusion: This study highlights the value of continuous RRS improvement in ARHs. Interventions led to notable enhancements, emphasizing the need for sustained efforts and future research on broader implementation.

A hypothesis concerning the nitrate ion.

The global electric circuit and the marine layer in coastal regions result in the presence of atmospheric negative polarity ions within the canopy of plants. This leads to the hypothesis:In the presence of negative polarity atmospheric ions plants activate a plant wide system to absorb and utilize these negative polarity ions.This plant wide system, termed Extracellular Transport System (ETS), is focused on nitrate movement. The object of this paper is to verify the existence of ETS by characterizing 1) how ETS absorbs ion from the atmosphere and 2) within the plant how ETS moves ions from source to destination. Over the past 2-years characteristics of ETS were examined in pecans, pistachios, lemons, wine grapes, cotton, corn, avocados and chili peppers in production agriculture fields in Arizona and California. Nitrate movement was separated into three physical locations: Location I, in the atmosphere outside the plant; Location II, in the interfacial volume between the atmosphere and the plant surface; Location III, in the plant itself. The paper is divided into three parts. Each part is concerned with a particular location of nitrate movement. The major tool of verification is presentation of simultaneous patterns of nitrate ion arrival rate on a simulated plant surface and subsequent movement of nitrate within the extracellular region of the plant. Use of this tool is illustrated in corn, lemons, chili peppers and avocados.A base functionality of ETS has been developed: ETS is a transient, plant wide system wherein 1) nitrate ions are putatively absorbed by a variety of epidermal structures including trichomes and transferred into the extracellular region, 2) hydrated pathways are produced in the extracellular region through which these nitrate ions pass 3) electrical potential gradients are created in the extracellular region which provide a force field to provoke movement of nitrate ions through these pathways. Anthropomorphic climate has three dimensions: light, temperature and moisture. Phytomorphic climate has five dimensions: light, temperature, moisture, earth tides and atmospheric ion presence. ETS is a natural adaptation of plants to the transient nature of atmospheric negative polarity ion presence. It provides a mechanism for plants to utilize this ubiquitous and renewable source of nitrate.

A hypothesis concerning callose.

Background Concept: Certain proteins and the glucose monomer have spacing of their carbonyl oxygen atoms that match the spacing of the oxygen atoms of hexagonal ice. This opens the possibility that a sequence of linked glucose residues may have a sequence of equally spaced carbonyl oxygen atoms. Hypothesis: Callose In plants is a duality consisting of the callose itself and a layer of ordered water whose oxygen atoms are hydrogen bonded to the carbonyl oxygen atoms in the callose. The atomic basis of the hypothesis is that the 1-3 linkage between glucose residues in callose results in equally spaced carbonyl oxygen atoms within and between residues. Properties of Callose/Ordered Water: The physical properties of the duality are the properties of callose itself: 1) it is immobile, 2) it can be created and dissolved, 3) it can exist at a submicrometer to micrometers space scale. The electrical properties of ordered water in a botanical platform are not known at the present time. They can be derived only from limited data in non biological platforms and inferences from the electrical properties of ice. These properties are 1) proton movement is governed by the Grotthuss mechanism, 2) there is insignificant movement of non-protonic ions and larger molecules through the ordered water, 3) proton movement is isotropic. Proposed Functionality of Callose/Ordered Water: Known locations of callose were examined theoretically to determine the functionality of a callose/ordered water duality. These locations were sieve plate pores, plasmodesmata and pollen tubes, stomatal guard cells, companion cell/sieve tube complex and micro and megasporocytes. Protonic Circuits: In a botanical context, protonic circuits at a single cell and supracellular level take the form of a proton microloop wherein callose/ordered water is one component in the loop. These circuits use both the enhanced proton mobility and the ion blocking ability of ordered water.

Analysis of western lowland gorilla (Gorilla gorilla gorilla) specific Alu repeats.

BACKGROUND: Research into great ape genomes has revealed widely divergent activity levels over time for Alu elements. However, the diversity of this mobile element family in the genome of the western lowland gorilla has previously been uncharacterized. Alu elements are primate-specific short interspersed elements that have been used as phylogenetic and population genetic markers for more than two decades. Alu elements are present at high copy number in the genomes of all primates surveyed thus far. The AluY subfamily and its derivatives have been recognized as the evolutionarily youngest Alu subfamily in the Old World primate lineage. RESULTS: Here we use a combination of computational and wet-bench laboratory methods to assess and catalog AluY subfamily activity level and composition in the western lowland gorilla genome (gorGor3.1). A total of 1,075 independent AluY insertions were identified and computationally divided into 10 subfamilies, with the largest number of gorilla-specific elements assigned to the canonical AluY subfamily. CONCLUSIONS: The retrotransposition activity level appears to be significantly lower than that seen in the human and chimpanzee lineages, while higher than that seen in orangutan genomes, indicative of differential Alu amplification in the western lowland gorilla lineage as compared to other Homininae.