RESUMO
To increase the efficiency of interpretation of mast cell's contribution to the state of a specific tissue microenvironment, it is necessary to detail the molecular composition of their secretome and analyze the pathways of degranulation. Developed method of combining immunomorphological and histochemical staining protocols contributes to the most objective detection of the integral level of tryptase expression in the intraorgan population of the skin mast cells. Novel technique for tryptase detection expands the possibilities of morphological analysis, provides researchers with additional data on the structure of the mast cell population and helps visualize the processing and cytological features and structural targets of tryptase during the development of adaptive and pathological reactions. Objective determination of the tryptase profile for organ-specific mast cell populations is in great demand in clinical practice for the interpretation of pathological processes, including inflammation and oncogenesis.
Assuntos
Regulação Enzimológica da Expressão Gênica , Mastócitos , Pele , Coloração e Rotulagem , Triptases/biossíntese , Animais , Masculino , Mastócitos/citologia , Mastócitos/enzimologia , Especificidade de Órgãos , Ratos , Ratos Wistar , Pele/citologia , Pele/enzimologiaRESUMO
The authors made an analysis of their first 100 transplantations of cadaveric liver made at the period from 1998 through 2011. Postoperative complications and long-term results of transplantations at the period to 13 years are described. Cumulative survival up to 12 months was 91%, to 36 months--83%. Retransplantation of the liver was performed on 5 patients, 2 of them being successful.
Assuntos
Doença Hepática Terminal/cirurgia , Transplante de Fígado , Complicações Pós-Operatórias/prevenção & controle , Coleta de Tecidos e Órgãos , Adulto , Feminino , Humanos , Terapia de Imunossupressão/métodos , Estimativa de Kaplan-Meier , Transplante de Fígado/efeitos adversos , Transplante de Fígado/métodos , Transplante de Fígado/mortalidade , Masculino , Pessoa de Meia-Idade , Monitorização Imunológica , Seleção de Pacientes , Reoperação , Coleta de Tecidos e Órgãos/métodos , Coleta de Tecidos e Órgãos/tendências , Resultado do TratamentoAssuntos
Cateterismo/métodos , Ducto Colédoco , Constrição Patológica , Drenagem/métodos , Transplante de Fígado/efeitos adversos , Complicações Pós-Operatórias/cirurgia , Anastomose Cirúrgica/efeitos adversos , Anastomose Cirúrgica/métodos , Colangiografia , Ducto Colédoco/patologia , Ducto Colédoco/fisiopatologia , Ducto Colédoco/cirurgia , Constrição Patológica/etiologia , Constrição Patológica/fisiopatologia , Constrição Patológica/cirurgia , Feminino , Humanos , Cirrose Hepática/cirurgia , Transplante de Fígado/métodos , Stents , Resultado do Tratamento , Adulto JovemRESUMO
The novel metalloporphyrins (M=HH, Fe, Mn, Co, Cu, Zn) bearing 2,6-di-tert-butylphenol pendants as antioxidant substituents, and a long chain hydrocarbon palmitoyl group have been synthesized. The oxidation of compounds by PbO2 leads to the formation of the corresponding 2,6-di-tert-butylphenoxyl radicals studied by EPR. The activity of porphyrins in lipid peroxidation has been examined using (1) in vitro lipid peroxidation induced by tert-butylhydroperoxide in respiring rat liver mitochondria, (2) in vitro lipid peroxidation in liver homogenates of Wistar strain rats, and (3) a model process of peroxidation of (Z)-octadec-9-enic (oleic) acid as a structural fragment of lipids. The activity of these compounds depends dramatically on the nature of metal and might be changed from antioxidative (M=HH, Mn, Cu, Zn) to indifferent (M=Co), and to pro-oxidative one (M=Fe). The anti- or pro-oxidative action of these compounds may be derived from the concurrence between the involvement of 2,6-di-tert-butylphenol pendants acting as radical scavengers and redox active metal center promoting oxidation processes. The results of this study suggest that the polytopic compounds combining in one molecule 2,6-di-tert-butylphenol pendants, metalloporphyrin moiety, and a palmitoyl group, are membrane active compounds and might be studied in an effort to find novel pharmaceutical agents.
Assuntos
Antioxidantes/síntese química , Antioxidantes/farmacologia , Metaloporfirinas/síntese química , Metaloporfirinas/farmacologia , Ácidos Palmíticos/química , Fenóis/química , Animais , Cobalto/química , Cobre/química , Espectroscopia de Ressonância de Spin Eletrônica , Ferro/química , Peroxidação de Lipídeos/efeitos dos fármacos , Manganês/química , Membranas Mitocondriais/efeitos dos fármacos , Ácido Oleico/química , Ácidos Palmíticos/farmacologia , Ratos , Ratos Wistar , Zinco/químicaRESUMO
New medicinal plant preparations of polyphenol nature, representing the derivatives of bioflavonoids (piflamin) and ellagotannins (altan and ellagic acid) were experimentally studied. The drugs exhibited antioxidant properties, which were manifested by inhibition of a pathological lipid peroxidation, restoration of the functional activity of the antioxidant system components, and stabilization of the hepatocyte membranes.
Assuntos
Antioxidantes/farmacologia , Ácido Elágico/farmacologia , Flavonoides/farmacologia , Taninos/farmacologia , Animais , Intoxicação por Tetracloreto de Carbono/metabolismo , Membrana Celular , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Feminino , Hepatócitos/efeitos dos fármacos , Hepatócitos/ultraestrutura , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/ultraestrutura , Masculino , Camundongos , RatosAssuntos
Antioxidantes/farmacologia , Fígado/efeitos dos fármacos , Animais , Antioxidantes/uso terapêutico , Glutationa/metabolismo , Humanos , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/metabolismo , Hepatopatias/tratamento farmacológico , Hepatopatias/metabolismo , Lipídeos de Membrana/metabolismo , Fosfolipídeos/metabolismoRESUMO
The hepatoprotective properties of the flavonoid preparation piflamine of field-peas grass were studied on a model of experimental paracetamol liver damage. Piflamine was found to normalize the parameters of carbohydrate, protein, and lipid metabolism, increase the activity of the antioxidant system, and restore the processes of bile production and bile secretion. The drug is prospective due to its quite cheap and available source of raw materials.