RESUMO
The Toxi-titer microtiter plate system (Bartels Immunodiagnostic Supplies, Inc., Bellevue, Wash.) is a simplified procedure for detecting the cytotoxin produced by Clostridium difficile in stool filtrates. In a parallel study of 74 stool specimens, results from the Toxin-titer system compared favorably with those from the conventional system. Our experience with the Toxin-titer system in testing 540 stool specimens was, in general, satisfactory, although a few problems with toxin control did occur. The method requires no specialized experience in tissue culture techniques and can therefore be adopted for routine use in clinical laboratories.
Assuntos
Proteínas de Bactérias , Toxinas Bacterianas/análise , Clostridium , Enterocolite Pseudomembranosa/diagnóstico , Fezes/análise , Linhagem Celular , Sobrevivência Celular , Estudos de Avaliação como Assunto , Fezes/microbiologia , Humanos , Kit de Reagentes para DiagnósticoRESUMO
The purpose of this article is to relate the process of designing a new Clinical Pathology Department laboratory at the National Institutes of Health and the assessment of the resultant open laboratory design based on the response of the laboratory staff to two questionnaires. The responses indicated acceptance and approval of the large open area, work area design, carpeted floor, and laboratory environment. It is the authors' hope that this information will be of help to other pathology departments who are involved in designing or remodeling a new or existing laboratory.
Assuntos
Arquitetura de Instituições de Saúde/normas , Departamentos Hospitalares , Laboratórios , Serviço Hospitalar de Patologia , Participação da Comunidade , Pisos e Cobertura de Pisos , Departamentos Hospitalares/organização & administração , Humanos , Satisfação no Emprego , Laboratórios/organização & administração , National Institutes of Health (U.S.) , Ruído Ocupacional/prevenção & controle , Serviço Hospitalar de Patologia/organização & administração , Inquéritos e Questionários , Estados UnidosRESUMO
A rapid entry mechanism for an anaerobic glove box is described. The entry port is practical for entry or removal of one or two items and requires only seconds to operate. No deleterious effect on isolation or growth of anaerobes has been encountered since installation of the rapid entry port 3.5 years ago. Details for its construction are given.
