Tetracycline removal from wastewater via g-C3N4 loaded RSM-CCD-optimised hybrid photocatalytic membrane reactor.

In this study, a split-type photocatalytic membrane reactor (PMR), incorporating suspended graphitic carbon nitride (g-C3N4) as photocatalyst and a layered polymeric composite (using polyamide, polyethersulfone and polysulfone polymers) as a membrane was fabricated to remove tetracycline (TC) from aqueous solutions as the world's second most used and discharged antibiotic in wastewater. The photocatalyst was synthesised from melamine by ultrasonic-assisted thermal polymerisation method and, along with the membrane, was characterised using various methods, including Brunauer-Emmett-Teller analysis (BET), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD), Field emission scanning electron microscopy (FESEM), and Ultraviolet-visible spectroscopy (UV-Vis). The PMR process was optimised, using Design-Expert software for tetracycline removal in terms of UV irradiation time, pH, photocatalyst loading, tetracycline concentration, and membrane separation iteration. It was revealed that a membrane-integrated reactor as a sustainable system could effectively produce clean water by simultaneous removal of tetracycline and photocatalyst from aqueous solution. The maximum removal of 94.8% was obtained at the tetracycline concentration of 22.16 ppm, pH of 9.78 with 0.56 g/L of photocatalyst in the irradiation time of 113.77 min after six times of passing membrane. The PMR system showed reasonable reusability by about a 25.8% drop in TC removal efficiency after seven cycles at optimal conditions. The outcomes demonstrate the promising performance of the proposed PMR system in tetracycline removal from water and suggest that it can be scaled as an effective approach for a sustainable supply of antibiotic-free clean water.

Type-1 α-Fe2O3/TiO2 photocatalytic degradation of tetracycline from wastewater using CCD-based RSM optimization.

Antibiotic pollution in water is a growing threat to public health and the environment, leading to the spread of antimicrobial-resistant bacteria. While photocatalysis has emerged as a promising technology for removing antibiotics from water, its limited efficiency in the visible light range remains a challenge. In this study, we present a novel method for the photocatalytic degradation of tetracycline, the second most commonly used antibiotic worldwide, using α-Fe2O3/TiO2 nanocomposites synthesized via rapid sonochemical and wet impregnation methods. The nanocomposites were characterised and tested using a range of techniques, including BET, TEM, FTIR, XRD, FESEM, EDS, and UV-Vis. The RSM-CCD method was also used to optimize the degradation process by varying four key variables (initial concentration, photocatalyst quantity, irradiation time, and pH). The resulting optimized conditions achieved a remarkable degradation rate of 97.5%. We also investigated the mechanism of photodegradation and the reusability of the photocatalysts, as well as the effect of light source operating conditions. Overall, the results demonstrate the effectiveness of the proposed approach in degrading tetracycline in water and suggest that it may be a promising, eco-friendly technology for the treatment of water contaminated with antibiotics.

Waste-to-Resource: New application of modified mine silicate waste to remove Pb2+ ion and methylene blue dye, adsorption properties, mechanism of action and recycling.

Currently, heavy metals and dyes are some of the most critical pollutants in the aquatic environment. So, in this paper "waste-to-resource conversion", as a new application of modified mine silicate waste to remove Pb2+ ion and methylene blue (MB) dye, adsorption properties, mechanism of action and recycling were studied. Silicate wastes are located in the alteration zone and the margin of molybdenum ore, these wastes are under the influence of hydrothermal solutions which are impregnated with iron and manganese ions. Hence, acid and base modifications have been commonly used. So, in this study, a highly porous nanostructure of modified silicate waste was used to remove MB and Pb2+ ion, in subsequent to our previous study on the application of the raw material of the same in the removal of malachite green. Acid, base, and acid/base treatments were used to activate and modify the adsorbent. Results show a significantly higher potential of modified adsorbent in the removal of MB and Pb2+ compared to the raw material. According to the isotherm and kinetic studies for MB and Pb2+ the Langmuir and Temkin and pseudo-second-order models were investigated with experimental data. Modified nanomaterial was used for several adsorption and desorption processes, without a significant decrease in the capability of the adsorbent in the removal of MB and Pb2+ pollutants. Leached iron and manganese ions (as production of modification) are deposited in the form of sludge using a simple pH adjustment and precipitation process and can be used to recover iron and manganese metals in the long run. The comparison of monolayer adsorption capacity using for Pb2+ ion and MB dye are as ((untreated SW: 29.41, 1.05); (NaOH treated: 21.74, 100); (Nitric Acid treated: 16.67, 142.86); (Citric Acid treated: 40, 125); (Nitric/Citric Acids treated: 15.63, 111.11) and (Nitric/Citric Acids/NaOH treated: 15.15, 83.33)), respectively. Higher adsorption capacity and re-generable properties of this adsorbent suggest the usage of this natural and abundant mine waste to treat wastewater containing toxic elements and dyes.

Heme degradation and iron release of hemoglobin and oxidative stress of lymphocyte cells in the presence of silica nanoparticles.

Silica nanoparticles (SiO2 NPs) have been widely used in the medical and food sciences. However, their toxic effects against bio-macromolecules and cells are not well understood. The present study was aimed to investigate the adverse effects of fabricated SiO2 NPs on the human hemoglobin (Hb) by FTIR, CD, fluorescence, and UV-vis spectroscopic techniques. Moreover, the toxic effects of SiO2 NPs on the human lymphocyte cell was assessed by trypan blue, reactivate oxygen species (ROS), and apoptosis assays. It was shown that synthesized SiO2 NPs have an amorphous structure with dominant size of around 20-30 nm. FTIR results showed that SiO2 NPs bind to Hb and induce significant structural changes on the native structure of Hb. Near CD spectroscopy depicted that SiO2 NPs induced tertiary structural changes and heme displacement. Fluorescence spectroscopy demonstrated the production of heme degradation species in the Hb solution after interaction with SiO2 NPs. UV-vis spectroscopy experiment indicated the release of iron form Hb after interaction with SiO2 NPs in a concentration dependent manner. Live-dead staining, ROS detection and flow cytometry analysis revealed that human lymphocyte was sensitive towards the toxicity of SiO2 NPs in a ROS-mediated apoptosis mechanism. In conclusion, SiO2 NPs exhibited concentration-dependent toxicity.