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Arch Razi Inst ; 75(1): 39-46, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-32292001

RESUMO

The present study was conducted as the first molecular detection of Anaplasma species in tick samples based on the sequencing of major surface proteins 4 (msp4) gene fragments in different parts of Iran. A total of 130 tick specimens were collected from Hormozgan, Lorestan, and Guilan, Iran, within 2015 to 2017. Hyalomma asiaticum, Hyalomma dromedarii, Rhipicephalus sanguineus, and Rhipicephalus (Boophilus) species were identified in different geographical regions. An amplicon of 464-bp msp4 of Anaplasma was amplified using polymerase chain reaction in various tick species. Three sequences, including one Anaplasma marginale from R. (Boophilus) species and two Anaplasma ovis from Rhipicephalus sanguineus, were obtained after sequencing. It is concluded that bovine and ovine anaplasmosis agents are present in tick samples in Iran. The use of the gene families of six major surface proteins for the detection of various Anaplasma species is recommended.


Assuntos
Anaplasma marginale , Anaplasma ovis , Ixodidae , Animais , Anaplasma marginale/isolamento & purificação , Anaplasma ovis/isolamento & purificação , Irã (Geográfico) , Ixodidae/microbiologia , Reação em Cadeia da Polimerase , Rhipicephalus/microbiologia , Rhipicephalus sanguineus/microbiologia
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