Survey dataset on the performance of combined process of coagulation and ceramic membrane for the removal of reactive black19.

The aim of data article was to evaluate effectiveness of the combined process of coagulation, flocculation and filtration to remove reactive black 19 from solution aqueous. In this data article, alum and ferric chloride were selected as a coagulant for instability of color before ceramic membrane. All experiments were performed in batch conditions. The results showed that more than half of the ceramic membrane is made of silica. The BET surface area of the ceramic membrane was 1.877 m2 g-1. The size of RB19, alum, and ferric chloride was 996.2 mm, 1216 mm, and 86.26 mm, respectively. Also, the zeta potential of RB19, alum, and ferric chloride was 20.7 mV, 1.59 mV, and 34.7 mV, respectively. The findings showed that the best pH to remove the RB 19 by alum and ferric chloride was less than 7. With increasing concentrations of alum and chlorine ferric the removal of RB 19 increased. For RB19 concentration of 10 mg l-1, with increasing time from 5 to 15 min, the removal efficiency for alum and ferric were 61-63% and 82-87%, respectively.

Design of a biological method for rapid detection of presence of PCR inhibitors in aged bone DNA.

BACKGROUND: Molecular human identification is one of the most important tests performed in forensic laboratories. Some of these tests are applied for identification of human remains from natural disasters, wars, etc., but problems may occur as a result of DNA degradation and external DNA contamination. We investigated effects of bacterial DNA on identifying the presence or absence of PCR inhibitors in aged bone DNA. METHODS: DNA samples were extracted from blood, bone remains and Escherichia coli. These DNA were amplified using human and bacterial specific primers. RESULTS: Using different blood, aged bone, and bacterial DNA dilutions along with PCR based methods; we checked their positive, negative effects, or detecting presence of inhibitors in aged bone DNA by PCR method. CONCLUSIONS: Our observation indicated that the addition of bacterial DNA could be a valid biological method for testing the quality of bone DNA to enable us to obtain a usable profile for the identification of human remains. This method will help to test the presence of inhibitors, quantity or even quality of DNA which are of importance in profiling archeological remains. Our method will help to determine if PCR failure is due to presence of inhibitors or lack of amplifiable DNA either because of degradation, minute amount or absence of human DNA.