Antileishmanial effect of podophyllotoxin and podophyllin on Leishmania major in vitro and in vivo.

Therapeutic research is very important in the prevention and treatment of leishmaniasis due to problems such as drug resistance, scarring and disease recurrence. The aim of this study was to determine how Leishmania major responds to the anti-leishmaniasis properties of podophyllotoxin and podophyllin. Cultured Leishmania promastigotes were exposed to different concentrations of podophyllotoxin and podophyllin for 24 and 48 h. Then, during the animal phase, Balb/c mice were experimentally injected with Leishmania promastigotes. After wounding, the effects of 0.5% podophyllotoxin and 25% podophyllin on reducing wound diameter and the number of amastigotes in the wound were evaluated. Podophyllotoxin and podophyllin were 83% and 59% lethal to Leishmania major promastigotes at the highest concentrations (200 µg/ml) and time (48 h). In the in vivo study, the mean lesion diameter at the end of treatment in the negative control group was 15.10 mm compared to 14.21 mm and 11.55 mm in the 25% podophyllin and 0.5% podophyllotoxin groups, respectively. Although both agents reduced the size of mice wounds and the number of amastigotes in the wounds, podophyllotoxin was more effective in this regard. Based on the results, podophyllotoxin and podophyllin can be used as leishmaniasis drugs after further research.

Characterizing the Oil-like and Surfactant-like Behavior of Polar Oils.

In this work, a bifunctional model was developed to fit and predict the phase inversion point (PIP) of microemulsions containing polar oils. This model incorporated the hydrophilic-lipophilic difference (HLD) equations, where HLD = 0 at the PIP. The model uses a Langmuir isotherm to account for the interfacial segregation of polar oils as a function of their concentration in the bulk oil phase. The segregated polar oil was treated as being surfactant-like, having a characteristic curvature (Cc). The polar oil in the bulk oil phase was characterized via an equivalent alkane carbon number (EACN). The Cc value was obtained considering deviations in the PIP at low polar oil concentrations. The EACN was determined considering PIP deviations at high polar oil concentrations. Naphthenic acid and dodecanol were used as model polar oils mixed with ionic and nonionic surfactants and nonpolar oils. The EACN of the polar oil was shown to be independent of the EACN of the nonpolar oil and likely independent of the surfactant. The Cc for dodecanol was likely independent of the surfactant used. For naphthenic acid, the Cc was independent of the nonpolar oil, and within a certain surfactant type (ionic, nonionic, or extended ionic), it was likely independent of the surfactant. For the naphthenic acid systems, the segregation predicted via the bifunctional model was consistent with experimental measurements of this segregation. Given that the bifunctional model only involves phase inversion experiments, it is a convenient method to determine the oil-like and surfactant-like nature of polar oils.