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1.
New Microbiol ; 34(4): 403-8, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22143814

RESUMO

Among 97 bacterial isolates, 74 strains of Staphylococcus spp developed from 95 swabs taken from skin lesions in dogs. Twenty-eight staphylococcal strains resistant to methicillin and/or oxacillin were identified and mecA expression was confirmed for 14 of these strains. S. aureus and S. intermedius group (SIG) strains were particularly relevant in our cases due to their antibiotic resistance leading to an increased veterinary and public health risk. We suggest a diagnostic protocol based on cytological examination, bacterial identification to species level, and antibiotic sensitivity testing prior to prescribing antibiotic treatment for canine skin diseases.


Assuntos
Antibacterianos/farmacologia , Dermatite , Doenças do Cão/tratamento farmacológico , Doenças do Cão/microbiologia , Infecções Cutâneas Estafilocócicas , Staphylococcus , Animais , Proteínas de Bactérias/genética , Dermatite/tratamento farmacológico , Dermatite/microbiologia , Dermatite/veterinária , Cães , Farmacorresistência Bacteriana/genética , Proteínas de Ligação às Penicilinas , Infecções Cutâneas Estafilocócicas/tratamento farmacológico , Infecções Cutâneas Estafilocócicas/microbiologia , Infecções Cutâneas Estafilocócicas/veterinária , Staphylococcus/efeitos dos fármacos , Staphylococcus/genética , Staphylococcus/isolamento & purificação , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/genética , Staphylococcus aureus/isolamento & purificação , Staphylococcus epidermidis/efeitos dos fármacos , Staphylococcus epidermidis/genética , Staphylococcus epidermidis/isolamento & purificação , Staphylococcus hominis/efeitos dos fármacos , Staphylococcus hominis/genética , Staphylococcus hominis/isolamento & purificação , Staphylococcus intermedius/efeitos dos fármacos , Staphylococcus intermedius/genética , Staphylococcus intermedius/isolamento & purificação
2.
Reprod Biol Endocrinol ; 7: 138, 2009 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-19948023

RESUMO

BACKGROUND: Lichtheimia corymbifera (previously Absidia corymbifera) is a filamentous zygomycetes belonging to the order Mucorales and to the family Lichtheimiaceae. Members of genus Lichtheimia spp. are cosmopolitan and ubiquitous in nature. Lichtheimia corymbifera is a recognized agent of diseases in man and animals. In cattle it causes abortion and mastitis. Three cases of bovine abortion occurred in a herd located in the Po Valley. Serological examinations were performed on fetal and mother's blood. One of the aborted fetus was referred to our laboratory. The paper describes the isolation and characterization of Lichtheimia corymbifera from a bovine aborted fetus. METHODS: Serological examinations were performed on fetal and mother's blood. Lesions on fetal tissues and placenta leaded the diagnostic suspect towards a mycotic aetiology. Tissues were then put in culture, and at the same time an histological examination was performed, together with bacteriological and virological tests. The isolate from placenta and fetal tissues was identified and characterized by PCR and RFLP, using the ITS region as a target sequence and AclI restriction site within the amplicon to distinguish Lichtheimia corymbifera among the other fungi. RESULTS: Serological, bacteriological and virological tests gave aspecific results. Histological examination evidenced numerous PAS positive hyphae within the necrotic cotiledons and numerous fungal nonseptate hyphae to the GMS stain. Colonies with typical morphological features of fungi grew up on Sabouraud agar from fetal skin and placenta. On the developed colonies the microscopic examination has shown a large number of nonseptate hyphae and sporangia consistent with Mucorales. PCR and RFLP allowed the identification of the isolate as Lichtheimia corymbifera. CONCLUSION: The present report describes the isolation and the molecular characterisation of a fungal isolate from bovine aborted fetus and placenta. The diagnostic protocol allowed to identify and characterise the strain. This is the first isolation in Italy of Lichtheimia corymbifera in a bovine aborted fetus.


Assuntos
Aborto Espontâneo/etiologia , Aborto Animal/etiologia , Absidia/isolamento & purificação , Mucormicose/complicações , Mucormicose/microbiologia , Aborto Espontâneo/microbiologia , Aborto Animal/microbiologia , Animais , Bovinos , Doenças dos Bovinos/etiologia , Doenças dos Bovinos/microbiologia , Doenças dos Bovinos/patologia , Feminino , Mucormicose/patologia , Placenta/microbiologia , Placenta/patologia , Gravidez
3.
New Microbiol ; 29(3): 211-4, 2006 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-17058789

RESUMO

Coxiella burnetii, the agent of Q fever in humans, has been associated with abortion in cattle. In this study 650 sera from cattle with abortion and 600 randomly-selected control sera were examined for antibodies to C. burnetii by ELISA. Two hundred and ninety-two (44.9%) out of 650 animals which experienced abortion were seropositive versus 132 (22%) out of 600 of the control group. A statistically significant difference resulted from the comparison of the seroprevalence of aborted cattle with that of controls (p < 0.001). Moreover, a significant higher prevalence was disclosed in cattle which aborted during late gestation (p < 0.002) and in the autumn (p < 0.001).


Assuntos
Aborto Animal/microbiologia , Doenças dos Bovinos/microbiologia , Coxiella burnetii/isolamento & purificação , Aborto Animal/epidemiologia , Animais , Anticorpos Antibacterianos/sangue , Bovinos , Doenças dos Bovinos/epidemiologia , Coxiella burnetii/imunologia , Ensaio de Imunoadsorção Enzimática , Feminino , Itália/epidemiologia , Gravidez , Estudos Soroepidemiológicos
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