RESUMO
A dimer of Pd(II), [(bpy)Pd(µ-OH)2Pd(bpy)]2+, (complex 1) (where bpy = 2,2'-bipyridyl) has been synthesized at physiological pH (7.4) and characterized by electronic spectroscopy, electrospray ionization mass spectrometry (ESI-MS) spectroscopy, and Fourier transform infrared (FT-IR) analysis. Reaction kinetics of 1 with glycine (L1H), l-glutamic acid (L2H), and l-arginine (L3H) were investigated in an aqueous medium at pH of 7.4 and constant ionic strength via a spectrophotometer as a function of temperature and different concentrations of substrate-complex and ligand. The interactions were supported by two discrete successive steps, i.e., ligand-dependent and ligand-independent steps. The equilibrium constant of complex formation (outer-sphere association) and the rate constant during complex-substrate-ligand interaction were calculated. The Eyring equation was applied to evaluate activation factors (ΔH and ΔS), and associative mechanisms of all reactions were proposed. Thermodynamic parameters (ΔH° and ΔS°) were also estimated from the standard plot of ln KE against 103/T. Spectroscopic titration of 1 at pH 7.4 in Tris-HCl buffer with calf thymus DNA, electronic emission titration with ethidium bromide (EtBr), antimicrobial activities, and an agarose gel electrophoresis run of 1 on pBR322 plasmid DNA have shown strong evidence of anticancer activity. Moreover, it has nontoxic water molecules as leaving groups.
RESUMO
Development of advanced biofuels such as bioethanol and biodiesel from renewable resources is critical for the earth's sustainable management and to slow down the global climate change by partial replacement of gasoline and diesel in the transport sector. Being a diverse group of aquatic micro-organisms, algae are the most prominent resources on the planet, distributed in an aquatic system, a potential source of bioenergy, biomass and secondary metabolites. Microalgae-based biofuel production is widely accepted as non-food fuel sources and better choice for achieving goals of incorporation of a clean fuel source into the transportation sector. The present review article provides a comprehensive literature survey as well as a novel approach on the application of microalgae for their simultaneous cultivation and bioremediation of high nutrient containing wastewater. In addition to that, merits and demerits of different existing conventional techniques for microalgae culture reactors, harvesting of algal biomass, oil recovery, use of different catalysts for transesterification reactions and other by-products recovery have been discussed and compared with the membrane-based system to find out the best optimal conditions for higher biomass as well as lipid yield. This article also deals with the use of a tailor-made membrane in an appropriate module that can be used in upstream and downstream processes during algal-based biofuels production. Such membrane-integrated system has the potential of low-cost and eco-friendly separation, purification and concentration enrichment of biodiesel as well as other valuable algal by-products which can bring the high degree of process intensification for scale-up at the industrial stage.
