[Biphasic action of human growth hormones, of hypophyseal origin or bacterial production, on the lipolysis of rat adipocytes in vitro]. / Action biphasique des hormones de croissance humaines, d'origine hypophysaire ou de fabrication bactérienne, sur la lipolyse d'adipocyte de rat "in vitro."

Actions of: human growth hormone resultant from hypophysis: HGH h (contaminated by beta LPH 1%) or produced by bacteria HGH b (pure), beta LPH resultant from human hypophysis, one of the somatomedins: multiplication stimulating activity (MSA), produced from a culture of rat hepatocytes, are considered in lipolysis of rat adipocytes "in vitro": basal, stimulated by epinephrine (0.1 and 1 microgram/ml), and when insulin is present (0.1 and 1 mUI/ml), during two periods I: 0-1 h 30, II: 1 h 30 - 4 h (hormonal addition at time 0). MSA appears to be antilipolytic during the two periods, between 0 and 4 h, with 500 ng/ml. A dose of 200 ng/ml is inactive. Beta LPH is lipolytic: 400 and 230 ng/ml, during phase I. At the ratio of the contaminate of HGH h 1000 ng/ml, resultant from hypophysis: 10 ng/ml, the action is less significant. Human growth hormones HGH h and HGH b appear to be: antilipolytic during the first period 0 1 h 30 with 1000 and 300 ng/ml, and, then lipolytic between 1 h 30 and 4 h with 1000, 300 and 100 ng/ml. The effect is pure with HGH b during the two periods, and, principally, during phase I when there is an absence of lipolytic contaminate. Biphasic action: antilipolytic, lipolytic, ascertained with a pure growth hormone: HGH b "in vitro" represents an effect "per se", non mediated by an eventual "in vivo" action of somatomedins.

[Effects of arginine, administered orally, on the endogenous secretion of the STH-somatomedin complex in young human volunteers]. / Effets de l'arginine, administrée par voie orale, sur la sécrétion endogène du complexe STH-somatomédines, chez l'homme volontaire à jeun.

Arginine, administered orally (arginine aspartate 37.5 g), induces upon voluntary fasting man laying down in bed (muscular rest), an increase of: 1) plasmatic HGH rate (radioimmunological) at 2 hours, 2) plasmatic SMs rate (biological: incorporation of 3H thymidine in human lymphocytes) at 8 hours, when compared with an equal administration of aminoacids without arginine or aspartate as placebo. Arginine enhances endogenous secretion of the complex HGH-somatomedines.

[Effects of insulin, somatomedin--a multiplication stimulating activity (MSA)--and transferrin, on the lipolysis of rat adipocytes in vitro]. / Effects de l'insuline, d'une somatomédine--facteur de multiplication cellulaire (MSA)--et de la transferrine, sur la lipolyse de l'adipocyte de rat in vitro.

Antilipolytic effect was researched when insulin (0.1 and 1 mIU/ml), MSA (200 and 500 ng/ml) and transferrin (2 and 5 micrograms/ml) were added to a suspension of freshly isolated rat adipocytes in vitro. Lipolysis was measured as glycerol secretion in the medium: micromoles/90 minutes/100 mg total lipids. Insulin (1 mIU/ml) reduced adrenalinic stimulation of lipolysis: A 1 microgram/ml (P less than 0.05). MSA 200 ng/ml had no effect. MSA 500 ng/ml reduced basal lipolysis and adrenalinic stimulation (P less than 0.05), and increased insulin-induced antilipolysis (P less than 0.05). Transferrin was active, only when insulin is present: antilipolysis increased (P less than 0.05).

[Potentiation by clofibride of the heparin-induced clearing reaction (intravascular hydrolysis of neutral lipids), in hyperlipemic rabbits]. / Potentialisation par le clofibride de la réaction de clarification à l'héparine (hydrolyse intravasculaire des lipides neutres), chez le lapin hyperlipémique.

A 16 days clofibride treatment in hyperlipaemic rabbit (fat supply), reduces hypertriglyceridemia and hypercholesterolemia. Heparine clearing reaction, performed on the 17th day, with the daily clofibride dose ingested in one time, is increased. The exaggerated intravascular lipids hydrolysis could explain, in part, the hypolipaemic action of clofibride.

[Effect of clofibride on the lipolysis of perirenal adipose tissue in hyperlipemic rabbits]. / Actions du clofibride sur la lipolyse du tissu adipeux périrénal chez le lapin hyperlipémique.

In hyperlipaemic rabbits (fat supply), perirenal adipose tissue lipolysis does not vary with one in vitro clofibride dose. After a 16 days pretreatment, one in vitro clofibride dose reduces the basal lipolysis and the lipolytic action of synacthene, and increases the antilipolytic effect of insulin. The reduction of the lipolysis of adipose tissue could explain, in part, the hypolipaemic action of clofibride.