The role of climatic changes and financial development to the ASEAN agricultural output: a novel long-run evidence for sustainable production.

This paper examines the impact of climate variables and financial development on agricultural value-added and cereal production in selected Southeast Asian economies from 1970 to 2016. The current research applies second-generation advanced techniques to accomplish robust and reliable outcomes. The findings from the FM-OLS estimation disclose that climatic factors, for instance, CO2e and average temperature, impact both agricultural value-added and cereal production negatively, and financial development has an inverted U-shaped influence on both agricultural value-added and cereal production. Meanwhile, other important factors, including cropped area, income level, and rural labor force, significantly improve agricultural value-added and cereal production. Furthermore, the FM-OLS estimator's D-H panel causality test outcomes are reliable. The findings of our study reveal that both the short- and long-run risks of climatic changes to the agricultural sector pose a large-scale threat to food security in Southeast Asian economies. As a result, a robust and stable financial development in terms of governance of climate change finance in the agriculture sector must be achieved to enhance farmers' ability to adapt to current and future climate change adverse impacts.

Epidemiological Features and Outcomes of COVID-19 in Patients With and Without Cardiovascular Disease.

BACKGROUND: Cardiovascular diseases (CVDs) are known as an important group of risk factor for progression of the Coronavirus-19 disease (COVID-19). The present study compared epidemiological features and outcomes in COVID-19 patients with CVDs versus those without CVDs. METHODS: This is a retrospective study performed on 1497 patients with CVDs and 26926 patients without CVDs, all of whom were confirmed to have COVID-19. All clinical signs and comorbidities were investigated in the subjects. Mann-Whitney U test and Pearson's Chi-square test were applied to compare mortality between the groups. Logistic regression was used to identify the predictors of mortality among patients. RESULTS: The mean age of COVID-19 patients with underlying CVD was 60 years. Totally, about 5.3% of the individuals under study had CVD. Also, 21.6% of all deaths occurred in COVID-19 patients with CVD. Cough, fever, shortness of breath, muscle pain, and underlying diseases such as diabetes, hypertension, chronic liver and kidney disease, chronic lung disease, and immunodeficiency were significantly higher in patients with CVD than those without CVDs. The odds of death in COVID-19 patients were 1.9 times higher with underlying CVD, 2.1 times with diabetes, 3.4 times with hypertension, 1.9 times with immunodeficiency, and 2.3 times with chronic liver and kidney disease. CONCLUSION: CVDs are a serious threat to COVID-19 patients because they increase mortality among these patients. As a result, preventive and therapeutic strategies must be developed for these vulnerable groups, who will be prone to higher mortality.

Measuring the Effects of Climate Change on Wheat Production: Evidence from Northern China.

The current study examines the long-run effects of climatic factors on wheat production in China's top three wheat-producing provinces (Hebei, Henan, and Shandong). The data set consists of observations from 1992 to 2020 on which several techniques, namely, fully modified OLS (FMOLS), dynamic OLS (DOLS), and canonical co-integrating regression (CCR) estimators, and Granger causality, are applied. The results reveal that climatic factors, such as temperature and rainfall, negatively influenced wheat production in Henan Province. This means that Henan Province is more vulnerable to climate change. In contrast, it is observed that climatic conditions (via temperature and rainfall) positively contributed to wheat production in Hebei Province. Moreover, temperature negatively influenced wheat production in Shandong Province, while rainfall contributed positively to wheat production. Further, the results of Granger causality reveal that climatic factors and other determinants significantly influenced wheat production in the selected provinces.

The Impact of Technological Progress and Climate Change on Food Crop Production: Evidence from Sichuan-China.

Agriculture is an integral sector in China mandated to feed over 1.3 billion of its people and provide essential inputs for many industries. Sichuan, a central grain-producing province in Southwest China, is a significant supplier of cereals in the country. Yet, it is likely to be threatened by yield damages induced by climate change. Therefore, this study examines the effects of technological progress (via fertilizers usage and mechanization) and climatic changes (via temperature and precipitation) on the productivity of main food crops, such as rice (Oryza sativa), wheat (Triticum aestivum), and maize (Zea mays) in Sichuan province. We employ the generalized method of moments (GMM) model to analyze Sichuan provincial data from 1980 to 2018. Our findings show a positive nexus between fertilizers use and yields of main food crops. Only rice and maize yields are significantly improved by mechanization. Increased average temperature reduces rice and wheat yields significantly. Rainfall is unlikely to have a significant impact on agricultural production. The study suggests that the Chinese government should consider revising its strategies and policies to reduce the impact of climate change on food crop production and increase farmers' adaptive ability.

Modeling the Impact of Climatological Factors and Technological Revolution on Soybean Yield: Evidence from 13-Major Provinces of China.

In recent years, the changing climate has become a major global concern, and it poses a higher threat to the agricultural sector around the world. Consequently, this study examines the impact of changing climate and technological progress on soybean yield in the 13 major provinces of China, and considers the role of agricultural credit, farming size, public investment, and power of agricultural machinery from 2000 to 2020. Fully modified ordinary least squares (FMOLS) and dynamic ordinary least squares (DOLS) are applied to assess the long-run effect, while Dumitrescu and Hurlin's (2012) causality test is used to explore the short-run causalities among the studied variables. The results revealed that an increase in the annual mean temperature negatively and significantly affects soybean yield, while precipitation expressively helps augment soybean yield. Furthermore, technological factors such as chemical fertilizers accelerate soybean yield significantly, whereas pesticides negatively influence soybean yield. In addition, farming size, public investment, and power of agricultural machinery contribute remarkably to soybean yield. The causality results endorse that chemical fertilizers, pesticides used, agricultural credit, public investment, and power of agricultural machinery have bidirectional causality links with soybean yield. This study suggests several fruitful policy implications for sustainable soybean production in China.

Does financial development mitigate the effects of climate variability on rice cultivation? Empirical evidence from agrarian economy.

This study is the first attempt to examine the effects of changing climate and financial development on rice cultivation in the context of agrarian economy like Thailand from 1969 to 2016. The current study also uses other important variables, such as cultivated area, organic fertilizers usage, and rural labor to determine the long-term connection amid variables. In this study, we applied several econometric techniques, for instance the autoregressive distributive lag-error correction model (ARDL-ECM), vector error correction model (VECM), impulse response functions (IMFs), and variance decomposition (VARD) method to estimate the most reliable and robust outcomes. The empirical results showed that in the long- and short-run, there is a reduction in rice cultivation as temperature increase. The carbon dioxide (CO2) positively affects rice cultivation in the long-run, while this association is negative in the short-run. The findings further revealed that in the long- and short-run, domestic credit provided by the financial sector significantly positive improved rice cultivation, while domestic credit to private sector by banks negatively affects rice cultivation. The important input factors, including cultivated area, organic fertilizers usage, and rural labor significantly positive contributed toward rice cultivation in the long- and short-run. The calculated long-run causal connection of all the studied variables with rice cultivation is validated. The estimated short-run causal relationship is unidirectional among temperature, CO2 emissions, financial development, rural labor, and rice cultivation. In addition, our outcomes are robust and also verified by IMFs and VARD method. The study offers some important policy suggestions to increase rice production with the help of sound and well-developed financial systems and climate controls.

Effects of Intergenerational Care Behavior on Residents' Nutrition Intake-Descriptive Statistical Analysis of Rural China Survey 2010-2014.

Nutritional status plays an indispensable role in enhancing human capital and preventing the return to poverty. In the context of the three-child policy and the aging society in China, intergenerational care will inevitably become a core component of family support. Thus, this paper evaluates the impacts of intergenerational care behavior on nutritional intake in Chinese rural residents from the perspective of household consumption. The study's data is from the China Family Panel Studies (CFPS) (2010-2014), and, the fixed effect model and analyses are applied to achieve the study's objective. The results reveal that rural residents with intergenerational family care behavior have significantly higher food diversity. Thus, Chinese rural residents with intergenerational care consumed 22.4% more food. A possible mechanism is that intergenerational care improves young mothers' labor participation and income, thus optimizing their dietary structure. Moreover, there was heterogeneity concerning the nutrition intake among rural residents in different geographic and family net income groups. Finally, the robust check is consistent with the baseline regression results. In summary, governments should fully affirm the elderly contribution to families to give full play to the elderly family benefit maximization.

Endorsing Sustainable Enterprises Among Promising Entrepreneurs: A Comparative Study of Factor-Driven Economy and Efficiency-Driven Economy.

Understanding business trails among promising aspirants may contribute to an actual motive for diminishing ecological tracks and escort to developing devotion toward deciding intentions across various entrepreneurial types and tiers solely from the sustainability domains. Therefore, this study endeavors to comprehend and seek to employ the Theory of Planned Behavior (TPB) to inspect the relationship between antecedents on sustainable enterprise intention and sustainable value creation. In this study, we used the convenience sampling method and the quantitative data of 1,070 respondents from Pakistan and China and applied a SmartPLS structural equation model and partial least square path modeling by mediational and multigroup analyses. Findings divulge that R2 (79.8%) value in the Pakistan sample of attitudes to sustainability, perceived entrepreneurial desirability (PED), and perceived entrepreneurial feasibility (PEF) was comparatively higher than in China. The R2 (75.6%) variance value on sustainable entrepreneurial intentions (SEI) was recorded higher in the Pakistani sample. However, the relationship of environmental values, self-efficacy, and extrinsic and intrinsic rewards show positive and significant mediational effects on both the economies of SEI. The findings disclosed an inconsistent character of extrinsic rewards, general self-efficacy, and job security depict negative significant impacts of aspirations on sustainable entrepreneurship (SE) among promising entrepreneurs on sustainability enterprises in both Pakistan and China. This study extends on existing entrepreneurship literature. Results supported the designed hypothesis and played a significant role in shedding light on an individual trait underpinning a career in a sustainable business start-up. The study looks at the issue from the viewpoint of sustainability domains. It seeks to determine the individual PED, PEF, and attitude toward sustainable entrepreneurship (ATSE) as the mediational variables. The study highlights the importance of work values in pursuing sustainability-oriented entrepreneurship programs for promising aspirants to improve their entrepreneurial skills and knowledge podium, which will encourage them to become sustainable future entrepreneurs. Furthermore, the study provides understandings for ratifying sustainable openings and debates the potential paths for sustainable business growth and opportunities among nascent entrepreneurs in both economies.

How do gender disparities in entrepreneurial aspirations emerge in Pakistan? An approach to mediation and multi-group analysis.

This cross-sectional study sought to identify gender differences in individual behavioral attitudes, personal traits, and entrepreneurial education based on planned behavior theory. The Smart partial least squares (PLS) structural equation model and PLS path modeling were used. A survey design was used to collect data from 309 samples using quantitative measures. The model was tested for validity and reliability and showed variance (full, R2 = 58.9% and split, R2 = 62.7% and R2 = 52.7%) in male and female model predictive power, respectively. Subjective norms (SN), personality traits (PT), and entrepreneurial education (EE) significantly impacted the male sample's intention. Females' intentions toward entrepreneurship was less affected by attitude toward behavior (ATB), subjective norms (SN), perceived behavioral control (PBC), and entrepreneurship education (EE). Further, attitudes, social norms, and behavioral controls as mediation variables indicate a significant and positive role of male and female intentions. These findings imply that behavioral beliefs (ATB, PBC, and SN) influence entrepreneurial intention-action translation. The results significantly supported the designed hypotheses and shed light on individual personality traits (PT) and entrepreneurship education (EE) underpinning enterprise intention. The study determined that EE and PT are the strongest predictors of intention, thus highlighting the role of these motives in the entrepreneurial process. This study contributes to the growing body of knowledge on youth entrepreneurs, expands our understanding of entrepreneurship as a practical career choice, and offers a novel account differentiating male and female PT. The drive to evaluate the effects of entrepreneurial intention among budding disparities in Pakistan requires a more profound knowledge of the aspects that endorse entrepreneurship as a choice of profession and enhances youth incentive abilities to engage in entrepreneurial activities based on exploitation.

Heavy Metal Resistance in Salmonella Typhimurium and Its Association With Disinfectant and Antibiotic Resistance.

Metals are widely used in animal feed for their growth-stimulating and antimicrobial effects, yet their use may potentially promote the proliferation of antibiotic resistance through co-selection. We studied the prevalence and associations of metal, antibiotic, and disinfectant resistances of 300 Salmonella Typhimurium isolates from pig meat, pig manure, chicken meat, poultry manure, and human stool from Sichuan, China. Seventy four percent of the 300 Salmonella Typhimurium isolates were considered resistant to Cu, almost 50% to Zn and Cr, over 25% to Mn and Cd, and almost 10% to Co. Most of the isolates carried at least one heavy metal resistance gene (HMRG). The Cr-Zn-Cd-resistance gene czcD was carried by 254 isolates and the Cu-resistance genes pcoR and pcoC by 196 and 179 isolates, respectively. Most of the isolates were resistant to at least one antibiotic and almost 80% were multidrug-resistant. The prevalence of resistance to six antibiotics was higher among the pig meat and manure isolates than among other isolates, and that of streptomycin and ampicillin were highest among the pig meat isolates and that of ciprofloxacin and ofloxacin among the pig manure isolates. From 55 to 79% of the isolates were considered resistant to disinfectants triclosan, trichloroisocyanuric acid, or benzalkonium chloride. The metal resistances and HMRGs were associated with resistance to antibiotics and disinfectants. Especially, Cu-resistance genes were associated with resistance to several antibiotics and disinfectants. The transfer of the Cr-Zn-Cd-resistance gene czcD, Cu-resistance gene pcoC, and Co-Ni-resistance gene cnrA into Escherichia coli and the increased Cu-resistance of the transconjugants implied that the resistance genes were located on conjugative plasmids. Thus, the excessive use of metals and disinfectants as feed additives and in animal care may have the potential to promote antibiotic resistance through co-selection and maintain and promote antibiotic resistance even in the absence of antibiotics.

Extracellular AGR2 activates neighboring fibroblasts through endocytosis and direct binding to ß-catenin that requires AGR2 dimerization and adhesion domains.

Anterior gradient 2 (AGR2) is often overexpressed in several types of cancer. AGR2 is cytoplasmic or secreted as an extracellular signal. Intracellular AGR2 properties and role in cancer have been well studied, but its extracellular function is largely unclear. It has been shown that extracellular AGR2 activates endothelial cells and fibroblasts in culture, but the mechanism of AGR2 signaling is not well elucidated. Here, we report that tumor secreted or externally added AGR2 translocates into cytoplasm by endocytosis, binds to ß-catenin and further co-translocates to the nucleus in NIH3T3 fibroblasts. Externally added AGR2 also increased ß-catenin expression, stability, and accumulation in the nucleus in both fibroblasts and cancer cells. External AGR2 rescued the expression of ß-catenin, which was suppressed by EGFR inhibitor AG1478 indicating an alternative pathway to regulate ß-catenin independent of EGFR signal. These effects were abolished when a monoclonal antibody against AGR2 was added to the experiments, confirming the effects are caused by AGR2 only. Putting together, our results show that extracellular AGR2 signaling pathway involves endocytosis mediated cellular translocation, direct binding and regulating ß-catenin nuclear accumulation. It is also a target against tumor initiated AGR2 signaling to form and maintain tumor microenvironment.

Metagenomic analysis revealed a wide distribution of antibiotic resistance genes and biosynthesis of antibiotics in the gut of giant pandas.

BACKGROUND: The gut microbiome is essential for the host's health and serves as an essential reservoir of antibiotic resistance genes (ARGs). We investigated the effects of different factors, including the dietary shifts and age, on the functional characteristics of the giant panda's gut microbiome (GPs) through shotgun metagenome sequencing. We explored the association between gut bacterial genera and ARGs within the gut based on network analysis. RESULTS: Fecal samples (n=60) from captive juvenile, adult, and geriatric GPs were processed, and variations were identified in the gut microbiome according to different ages, the abundance of novel ARGs and the biosynthesis of antibiotics. Among 667 ARGs identified, nine from the top ten ARGs had a higher abundance in juveniles. For 102 ARGs against bacteria, a co-occurrence pattern revealed a positive association for predominant ARGs with Streptococcus. A comparative KEGG pathways analysis revealed an abundant biosynthesis of antibiotics among three different groups of GPs, where it was more significantly observed in the juvenile group. A co-occurrence pattern further revealed a positive association for the top ten ARGs, biosynthesis of antibiotics, and metabolic pathways. CONCLUSION: Gut of GPs serve as a reservoir for novel ARGs and biosynthesis of antibiotics. Dietary changes and age may influence the gut microbiome's functional characteristics; however, it needs further studies to ascertain the study outcomes.

A mix-method investigation on acculturative stress among Pakistani students in China.

This article investigates acculturation stress among Pakistani students who are studying in Chinese universities, located in five provinces where international students are concentrated, with a mix-method approach. 203 students among 260 questionnaire recipients responded the online survey. When using the ASSIS (Acculturation Stress Scale for International Students) as instrument, the Principal Component Analysis Method and SPSS 20.0, we found that Pakistani students are under acculturative stress, 68.53%, 10.97% and 9.15% of them perceived discrimination, home sickness and perceived hate, and 5.25%, 3.11% and 2.58% of them fear, culture shock and guilt respectively. The qualitative segment of the study is consisted of 20 Pakistani students studying in 4 universities located in Wuhan city of Hubei capital enquiring through semi-structured interviews. The findings illustrate that Pakistani students in China are expressing their major concerns on culture shock, homesickness, food and language barriers while disconfirm ASSIS findings like perceived discrimination, hate, fear and guilt as factors responsible for acculturative stress. The study suggested that pre-departure orientation lectures about host country's cultural values and campus environment, and on-campus extra-curricular, cultural activities and maximum social interaction with local students can effectively acculturate students in new cultural setting, and can lower their acculturative stress.

Presence of heavy metal resistance genes in Escherichia coli and Salmonella isolates and analysis of resistance gene structure in E. coli E308.

OBJECTIVES: With the wide use of heavy metals as feed additives in animal production, little attention has been paid to heavy metal resistance in pathogenic bacteria. This study was performed to investigate the presence of heavy metal resistance genes (HMRGs) in Escherichia coli and Salmonella isolates and its correlation with disinfectant resistance genes (DRGs) and antibiotic resistance genes (ARGs). METHODS: HMRGs of 178 E. coli and 294 Salmonella isolated from chicken broiler farms and retail meat were detected by PCR. Minimum inhibitory concentrations (MICs) of heavy metals were determined by the broth microdilution method. The complete genome of E. coli E308, which had indications of multidrug resistance, was recovered and assembled using third-generation sequencing. RESULTS: The frequency of different HMRGs in E. coli and Salmonella ranged from 0.60-77.0% and 0.30-87.1%, respectively. MICs of heavy metals for E. coli and Salmonella ranged widely from ≤12.5 mg/L to 1600 mg/L. Moreover, HMRGs (zntA, arsB, merA, pcoR, pcoA, pcoC and chrA) were found to be significantly associated with one or more DRGs [sugE(c), emrE, mdfA, ydgE/ydgF, qacF, sugE(p) and qacEΔ1] and ARGs (sul1, sul2, sul3, tetA, tetB, tetC, blaTEM, blaSHV and blaCTX) (P < 0.05). CONCLUSION: This study demonstrated that HMRGs are widely present in E. coli and Salmonella isolated from chicken farms and retail meat. The association between HMRGs with DRGs and ARGs may lead to co-resistance to heavy metals and other antimicrobial agents.

Microscopic investigation of pollen morphology of Brassicaceae from Central Punjab-Pakistan.

The intention of the current study is to provide an account on the palynological features of Brassicaceae from Central Punjab-Pakistan as a basis for future studies. Different morpho-palynological characteristics both qualitative and quantitative were analyzed during this research which includes shape of pollen, diameter of pollen, P/E ratio, exine sculpturing, thickness of exine, type of pollen, shape and size of lumens, and thickness of murus. Taxonomic keys were also constructed based on pollen morphological characters for correct identification of species. This study aims to provide detailed information of pollen diversity and their exine structure based on both qualitative and quantitative characters by using Light microscopy and Scanning electron microscopy. Shape of pollen is mostly prolate, but some species also have sub-prolate to spheroidal prolate types. Exine ornamentation in most species was reticulate, whereas micro reticulate (one species) and coarsely reticulate (one species) exine also observed in some pollen. All the pollen mentioned in this study have tricolpate apertures. Variation found in thickness of exine and other characters proved to be helpful at generic and specific level. The results reinforced the significance of pollen morphological features of family Brassicaceae and aid for valuable taxonomic tool in plant systematics.

Acute poisoning in children.

OBJECTIVE: To determine the frequency, etiology, clinical presentation and outcome of acute poisoning in children presenting to a tertiary care hospital. DESIGN: Descriptive and observational study. PLACE AND DURATION OF STUDY: Emergency Department of the Children's Hospital and the Institute of Child Health, Lahore. Over five years from September 1998 to August 2003. MATERIAL AND METHODS: All cases of suspected or confirmed acute poisoning in children (1 month to 15 years) were retrospectively analyzed by retrieving data from hospital records and from patients files where possible. Variables like demographic data, season of presentation, type of agent, route of exposure and outcome were studied for all cases. Detailed files were available for 90 patients in whom place of exposure, risk factors and clinical features were also studied. RESULTS: Three hundred and forty-six cases of acute poisoning were registered during the study period, which constitute 0.93% of all ER admission. Most (59%) were less than 2 years of age with median age of 18 months (SD+ 32.6). Majority (80%) belonged to urban areas and presented during summer and monsoon season (57%). Pharmaceutical products were the leading cause (51%) followed by petroleum products (23%), chemicals (8.4%), household substances (7.6%) and unidentifiable agents in 10% cases. Anti-diarrhoeal drugs and kerosene oil were the two most frequent agents involved and ingestion remained the primary route of exposure. Gastrointestinal system was primarily involved in 40% patients. Eighty percent patients were discharged, 6% with residual damage while 11% patients died of poisoning or its complications. CONCLUSION: Acute poisoning carries a significant impact as regards morbidity and mortality. Awareness and education about the potential toxicity of commonly used drugs and household substances may help in reducing the burden of acute poisoning in children.